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  • 1
    Electronic Resource
    Electronic Resource
    Localisation of C-terminal gastrin immunoreactivity in gastrinoma cells (1985)
    Springer
    Virchows Archiv 406 (1985), S. 223-236 
    Details
    ISSN: 1432-2307
    Keywords: Gastrin ; Gastrinomas ; Ultrastructure ; Immunogold technique
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Localisation of C-terminal gastrin immunoreactivity has been studied, using the immunogold staining procedure, on ultrathin sections of 6 human gastrinomas conventionally processed for electron microscopy. The specific labelling, whose density depended on the mean diameter of the gold marker, was restricted to endocrine secretory granules. However, in poorly differentiated cells from malignant tumours, a number of granules remained unreactive. The labelling pattern depended also on the functional state of each cell. The immunoreactive granules showed various morphological features. A moderate number of gold particles was demonstrated over the floccular content of the infrequent diagnostic G-type granules. Non-diagnostic round granules of varying size and electron density were prevalent in most cells; their usually strong immunostaining allowed immediate recognition of cell specificity. Dense granules which were large in size and angular in shape and present in one case, were also intensely labelled. In the same tumour, unequal labelling occurred over polymorphous, often elongated granules, of varying size. Granules of different types, including intermediate forms, could be found in the same cell, indicating a spectrum of granule maturation towards well-defined types of the fetal or adult normal tissues. The present methodology would help to identify gastrin-producing cells in prospective or retrospective electron microscopy studies of multihormonal endocrine tumours.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00737088
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  • 2
    Electronic Resource
    Electronic Resource
    Storage and degradation of secretory proteins in adenomatous and secondary hyperplastic parathyroid cells (1989)
    Springer
    Virchows Archiv 415 (1989), S. 305-316 
    Details
    ISSN: 1432-2307
    Keywords: Parathyroid ; Storage granule ; Parathormone ; Chromogranine A ; Immunoelectron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Parathyroid hormone (PTH) and chromogranin A/secretory protein-I (SP-I) have been localized on immunoelectron microscopy in double-fixed tissues from adenomatous and secondary hyperplastic parathyroid glands. Storage organelles, identified on the basis of their consistent labelling, included two morphologically distinct varieties of granules/vesicles; the mature granules and the progranules. The former consisted of dense, mostly rounded, medium to large-sized bodies which were strongly labelled and predominant in the proximity of the cell membrane. The other variety of body included a spectrum of small pale vesicles/granules which were mainly located in the Golgi area. Because their morphology and their labelling pattern varied other bodies were assumed to be engaged in degradation or cleavage of the secretory proteins. These bodies comprised crinophagic structures, that is to say multivesicular bodies and large Golgi-related vesicles, as well as a number of atypical solid bodies. Whereas most of the granulated cells stored a mature or a maturing population of vesicles/granules, the process of maturation appeared to be either absent or incomplete in a number of cells from some glands. The major defects were frequently associated with an unusual labelling pattern of the Golgi area and selectively affected groups of cells from all the transitional oxyphil cell adenomas. The minor defects concerned individual cells of different types present in both categories of glands. The present data suggest that in hyperfunctioning glands, the type of hormone processing depends on the capacity of each cell in progranule maturation and that the maturation capacity may decrease dramatically in adenomatous or chronically hyperstimulated cells of the transitional oxyphil type.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00718633
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  • 3
    Electronic Resource
    Electronic Resource
    Electron microscope localisation of insulin-like immunoreactivity of conventionally processed human insulinomas (1988)
    Springer
    Virchows Archiv 412 (1988), S. 443-450 
    Details
    ISSN: 1432-2307
    Keywords: Insulin ; Insulinomas ; Ultrastructure ; Immunogold technique
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Localisation of insulin-like immunoreactivity has been studied using the immunogold staining procedure on thin sections of 6 human insulinomas, conventionally processed for electron microscopy. The labelling was restricted to the secretory granules. Depending on their morphology, these either resembled B-cell granules of human adult pancreas or belonged to the atypical (non-diagnostic) group. Within the former group, those with a crystalloid core or an amorphous dense or moderately dense core were strongly immunoreactive, whereas others, filled with a pale material, were poorly labelled. Most granules of this type were stored together within the heavily granulated cells of 3 insulinomas, presenting the classical features of clinical and biological behaviour and a typical light microscopic staining pattern. In contrast, the non-diagnostic granules, characterized by their smaller size, a very dense core and a thin halo, were mainly found within the poorly granulated cells making up the other tumours, and showed a very uneven labelling. Strongly labelled granules were found in one insulinoma that also belonged to the classical type; these were stored together with a few diagnostic granules within the same cells. Only poorly labelled atypical granules were present in two cases revealing a number of unusual features; these included moderate elevation of insulinaemia, uncertain tumour histology, as well as weak immunostaining for insulin/proinsulin and variable argyrophilia of the tumour in paraffin sections. These findings suggest that human insulinomas differ not only in storage capacity but also in their degree of granule maturation. This may involve some deficiency of either the prohormone conversion or the subsequent processing of the cleavage products.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00750578
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  • 4
    Electronic Resource
    Electronic Resource
    Agrobacterium‐mediated transformation of lavandin (Lavandula x intermedia Emeric ex Loiseleur) (1999)
    Springer
    Transgenic research 8 (1999), S. 335-347 
    Details
    ISSN: 1573-9368
    Keywords: Agrobacterium tumefaciens ; ß‐glucuronidase ; lamiaceae ; lavandin ; neomycin phosphotransferase II ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Lavandin (Lavandula x Emeric ex Loiseleur) is an aromatic plant, the essential oil of which is widely used in the perfume, cosmetic, flavouring and pharmaceutical industries. The qualitative or quantitative modification of its terpenes‐containing essential oil by genetic engineering could have important scientific and commercial applications. In this study, we report the first Agrobacterium tumefaciens‐mediated gene transfer into lavandin. The transformation protocol was optimized by lengthening precultivation and cocultivation periods and by testing five different bacterial strains. We obtained transformed callus lines at a frequency of 40–70 with strains AGL1/GI, EHA105/GI and C58/GI. Transgenic shoots were regenerated from these kanamycin resistant calli and rooted on selective medium with 150 mg l-1 kanamycin. The final percentage of transgenic plants obtained varied from 3 to 9, according to the strain used, within 6 months of culture. The presence of the introduced β‐glucuronidase and neomycin phosphotransferase II genes was shown both by PCR and Southern blot analysis. Transgene expression was investigated using histoenzymatic β‐glucuronidase assays, leaf callus assays and RT‐PCR. Results showed that both β‐glucuronidase and neomycin phosphotransferase II genes were expressed at a high level in at least 41 of the transgenic plants regenerated. This efficient transformation strategy could be used to modify some genetic traits of lavandin (flower colour, pathogens resistance) and to study the biosynthesis of the major monoterpene components of its essential oil (linalool, linalyl acetate, camphor and 1,8‐cineole).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008948113305
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  • 5
    Electronic Resource
    Electronic Resource
    Pulsed versus continuous wave Nd-YAG laser-induced necrosis: Comparison in the rat liver in vivo (1995)
    Springer
    Lasers in medical science 10 (1995), S. 47-53 
    Details
    ISSN: 1435-604X
    Keywords: Laser-tissue interaction ; Laser-induced lesion ; 1064 nm wavelength ; Continuous wave Nd-YAG laser ; Pulsed Nd-YAG laser
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract It is assumed that a pulsed Nd-YAG laser will avoid heat diffusion in tissue and thus produce more predictable and less deep lesions. The aim of this study was to compare lesions induced in an homogeneous tissue by the pulsed wave (PW) and the continuous wave (CW) modes of the Nd-YAG laser. Single laser shots were delivered to the liver surface of anaesthetized rats after laparotomy, in vivo. In experiment 1, the quartz fibre was handled close to the liver surface. Energies of 10, 20, 40 J were applied. In experiment 2, the quartz fibre was fixed at a distance from the liver and the laser beam was focused through a handpiece, to obtain a spot of 3 mm in diameter at the liver surface. Energies of 20, 40, 80 J were applied. In both studies, four or five shots were performed for each parameter with each laser mode. After excision of the liver, the maximal depth and width of the crater, the necrotic area and the total affected tissue were measured for each lesion. In experiment 1, there was no difference in any dimension of lesion between the two modes. In experiment 2, the only statistical difference was observed at the fluence of 566 J cm−2 where the necrotic area as well as the total lesion were deeper with the pulsed mode. This difference was not observed for the crater. In this experimental model depths and widths of the different layers of the lesion induced by the PW mode were comparable to those obtained with the CW mode. This PW mode of Nd-YAG laser does not prevent heat diffusion in tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02133164
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