ISSN:
1432-2099
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Physics
Notes:
Summary Mammalian cell mutants with defective repair of UV-induced DNA damage can be identified by the lack of unscheduled incorporation of radioactive thymidine into DNA after UV irradiation. With a specially designed windowlessMultiWireProportionalChamber (MWPC), colonies with absent or low incorporation of3H-thymidine can be precisely located within a population of normally labelled colonies. The main advantages of MWPC analysis over other more conventional methods for radioactive measurements are: (i), cells are not harmed by the analysis, so that previous replica plating of colonies becomes unnecessary; (ii), measurements are quantitative, hence discrimination between colonies is not subjective; (iii), location of unlabelled, non-repairing colonies is rapid and can be automatized. The method therefore appears suitable for the isolation of mammalian cell mutants with a block in DNA repair.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01323642
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