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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 155 (1991), S. 449-452 
    ISSN: 1432-072X
    Keywords: Aquaspirillum ; Conjugation ; Electroporation ; Magnetogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Electroporation methods and conjugal matings were used to transfer several plasmid vectors to Aquaspirillum dispar and Aquaspirillum itersonii. The incompatibility P class plasmid RP4 was conjugally transferred from Escherichia coli HB101 to these spirilla, and the transconjugants subsequently donated the molecule to plasmid-free E. coli and A. dispar strains via conjugal matings. High-voltage electrotransformation was used to transfer plasmids pUCD2, pSa151 and RP4 to A. dispar and A. itersonii, at efficiencies as high as 3×104 transformants per μg plasmid DNA. RP4 DNA isolated from spirillum hosts, but not RP4 from E. coli cells was successfully transferred to A. dispar and A. itersonii by electrotransformation, suggesting that modification and/or restriction activity may be present in these Aquaspirillum species.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-072X
    Keywords: Intercellular junctions ; Multicellularity in prokaryotes ; Bacterial magnetotaxis ; Ultrastructure ; Bacterial co-ordination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A many-called magnetotactic prokaryote obtained from brackish water was observed to possess intercellular connections at points of contact between the outer membranes of constituent cells. Each aggregate organism consisted of 10 to 30 individual Gram-negative cells containing material with the appearance of poly-β-hydroxybutyrate and magnetosomes of unusual arrangement, structure and composition. The aggregate, which possessed prokaryotic-type flagella arranged at the outwards surfaces of each cell, showed motility indicative of co-ordination between individual component cells. These results suggest that this organism could be a multicellular prokaryote.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 310 (1984), S. 405-407 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Fig. 1 Chain of magnetite particles imaged within an intact unstained A. magnetotacticum cell, showing a combination of large and small particles. Particle A has a characteristic morphology seen in many mature crystals (see Fig. 3). Particle ? is irregular in form and appears to be at a different ...
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Current microbiology 15 (1987), S. 121-127 
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The respiratory chain ofAquaspirillum magnetotacticum strain MS-1 cells denitrifying microaerobically included a-, a1-, b-, c-, cd1-, and o-type hemes. More than 85% of the total cytochromes detected were of the c type. Virtually all of the a and b types were detected in cell membranes, whereas 70% of the c-type hemes were soluble. Large quantities of soluble c-type hemes were released with periplasm by freezing and thawing cells. Soluble c551 occurred in two forms: as a single compound of apparent molecular weight of 17,000 daltons, which bound CO, and, together with d1 heme, as a component of nitrite reductase. Both a1-type hemes (which usually comprise part of the “low aeration” cytochrome oxidase) and o types (usually part of the “high aeration” oxidase) were simultaneously expressed in microaerobically grown denitrifying cells ofA. magnetotacticum; this indicated branching of the respiratory chain.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Current microbiology 17 (1988), S. 339-342 
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Iron reductase activity in cell extracts ofAquaspirillum magnetotacticum strain MS-1 (wild type) or nonmagnetotactic mutant strain NM-1A was located primarily in the periplasm. Cytoplasm contained 20%–35% and membrane fractions 3% of total iron reductase activity detected. Iron reduction was reversibly inhibited by oxygen, required β-NADH, and was enhanced by flavins. Reduced disulfide bonds and uncomplexed sulfhydryl groups were necessary for reductase activity. Respiratory inhibitors did not appreciably affect iron reductase activity. Iron complexed with quinic acid, dihydroxybenzoic acid, acetohydroxamic acid, citric acid, or deferrioxamine B was reduced by soluble iron reductases of strain MS-1.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 10 (1989), S. 223-237 
    ISSN: 0197-8462
    Keywords: geomagnetic field ; biogenic magnetite ; bacterial magnetotaxis ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Magnetotactic bacteria from freshwater and marine sediments orient and navigate along geomagnetic field lines. Their magnetotactic response is based on intracellular, single magnetic domains of ferrimagnetic magnetite, which impart a permanent magnetic dipole moment to the cell.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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