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  • 1
    Electronic Resource
    Electronic Resource
    Expression of the mouse testicular histone gene H1t during spermatogenesis (1996)
    Springer
    Histochemistry and cell biology 106 (1996), S. 247-251 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  The testicular H1 histone variant, H1t, is synthesized during spermatogenesis in mammalian male germ cells. In situ hybridization and immunohistochemical techniques were used to assign the expression of either the H1t mRNA or the H1t protein to specific cell stages of spermatogenesis. Our results show the presence of the H1t mRNA only in the late and mid-pachytene stages, whereas the protein occurs first in pachytene spermatocytes, and persists until later stages from round up to elongated spermatids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004180050039
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  • 2
    Electronic Resource
    Electronic Resource
    Spermatogenesis proceeds normally in mice without linker histone H1t (2000)
    Springer
    Histochemistry and cell biology 113 (2000), S. 433-442 
    Details
    ISSN: 1432-119X
    Keywords: Chromatin structure Linker histones H1t-deficient mice Spermatogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The histone gene H1t is expressed exclusively in pachytene spermatocytes of the testis. In this report we have eliminated the single copy H1t gene by homologous recombination from the mouse genome to analyse the function of the H1t protein during spermatogenesis. Mice homozygous for the mutated H1t gene locus developed normally and showed no anatomic abnormalities until the adult stage. In addition, H1t-deficient mice were fertile and reproduced as wild-type mice. The process of spermatogenesis and the testicular morphology remained unchanged in the absence of H1t. RNase protection analysis demonstrated that H1.1, H1.2 and H1.4 histone gene expression is enhanced during spermatogenesis in H1t-deficient mice.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004180000146
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  • 3
    Electronic Resource
    Electronic Resource
    Expression of the mouse histone gene H1t begins at premeiotic stages of spermatogenesis (1997)
    Springer
    Cell & tissue research 291 (1997), S. 127-132 
    Details
    ISSN: 1432-0878
    Keywords: Key words Histone H1t ; Spermatogenesis ; Immunogold staining ; In situ hybridization ; Western blot analysis ; Mouse (NMRI)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  The gene encoding H1t, a testicular variant of histone H1, is expressed in mammals during spermatogenesis. Northern blot and in situ hybridization has detected H1t mRNA only at the stage of pachytene spermatocytes. We have extended this analysis to more sensitive approaches and demonstrate, by RNase protection and electron-microscopic in situ hybridization, that H1t mRNA is detectable even in spermatogonia. Just a faint H1t band is seen in Western blots of nuclear protein from 9-day-old mice. This indicates that the H1t gene is expressed at premeiotic stages, albeit at a low level. In contrast to H1t mRNA, the H1t protein has not been detected in spermatogonia by electron microscopy after immunogold staining.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050986
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  • 4
    Electronic Resource
    Electronic Resource
    The haemochromatosis candidate gene HFE (HLA-H) of man and mouse is located in syntenic regions within the histone gene clusterSequence data reported in this article have been deposited with the EMBL/GenBank Data Libraries under Accession nos. Z92910 and Y12650. (1998)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 69 (1998), S. 117-126 
    Details
    ISSN: 0730-2312
    Keywords: haemochromatosis gene ; histone gene cluster ; YACs ; cosmid contig ; sequences ; species comparison ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: The HFE (HLA-H) gene is a strong candidate gene for hereditary haemochromatosis and was localized on the short arm of chromosome 6 to 6p21.3-p22. In addition, the sequence of the homologous mouse and rat cDNA and a partial sequence from the mouse gene have been reported recently. In this report, we describe the location of the human and the mouse HFE (HLA-H) gene within the histone gene clusters on the human chromosome 6 and the mouse chromosome 13. Both the human and the murine gene were located on syntenic regions within the histone gene clusters in the vicinity of the histone H1t gene. The genomic sequence of the human HFE (HLA-H) gene and the 3′ portion of the homologous mouse gene were determined. Comparison of the genomic sequences from man and mouse and the cDNA sequence from rat shows significant similarities, also beyond the transcribed region of the mouse gene. J. Cell. Biochem. 69:117-126, 1998. © 1998 Wiley-Liss, Inc.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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