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  • 1
    Electronic Resource
    Electronic Resource
    Inhibition of α-aminoadipate-semialdehyde dehydrogenase from Trichosporon adeninovorans bvy lysine and lysine analogues (1990)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 70 (1990), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The α-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31) of Trichosporon adeninovorans, an enzyme of lysine biosynthesis, was partially purified, some properties of the enzyme were studied and a novel regulatory pattern was found. The Km values of the enzyme were estimated to be 0.78 mM for α-aminoadipate, 1.0 mM for ATP, 0.23 mM for NADPH and 0.77 mM for MgCl2. It is demonstrated that the enzyme can be regulated by lysine and lysine analogues. l-Lysine (Ki of 0.09 mM), S-(B-aminoethyl)-l-cysteine (Ki of 0.007 mM and δ-hydroxylysine (Ki of 1.65 mM) inhibited the enzyme activity. The inhibition was competitive with respect to α-aminodipate and non-competitive with respect to both ATP and NADPH.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1990.tb03774.x
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  • 2
    Electronic Resource
    Electronic Resource
    A novel enzyme, L-lysine : pyruvate aminotransferase, catalyses the first step of lysine catabolism in Pichia guilliermondii (1988)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 49 (1988), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract A novel aminotransferase catalysing the first step of lysine catabolism, the oxidative transamination of the ?-group of L-lysine, was found and characterised in the yeast Pichia guilliermondii. The enzyme, L-lysine : pyruvate aminotransferase (Lys-AT), is strongly derepressed in cells grown on L-lysine as sole nitrogen source and its activity is highly specific for both L-lysine and pyruvate. We could successfully isolate a regulatory mutant which is unable to use lysine as sole nitrogen source based on its inability to derepress the Lys-AT.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1988.tb02716.x
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  • 3
    Electronic Resource
    Electronic Resource
    Threonine dehydratase activity from several yeast species is activated and affected by phosphate (1986)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 37 (1986), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The threonine dehydratase activity of several yeast species was examined. Of 14 species tested, Candida maltosa, Pichia guilliermondii, Pichia pinus and Yarrowia lipolytica were found to produce an enzyme whose activity was increased about 20-fold in the presence of phosphate ions. It is suggested that phosphate affects the allosteric properties of the enzyme and leads, thereby, to an alteration of the binding of threonine (substrate), isoleucine (negative effector), and valine (positive effector) as well as to an alteration of the effects of temperature on the enzyme including changes in temperature optimum, energy of activation, and heat inactivation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1986.tb01791.x
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  • 4
    Electronic Resource
    Electronic Resource
    The macroinvertebrate fauna of an acid-stressed headwater stream system in the Adirondack Mountains, New York (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Freshwater biology 15 (1985), S. 0 
    Details
    ISSN: 1365-2427
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SUMMARY. 〈list xml:id="l1" style="custom"〉1The Silver Run system in the Adirondack Mountains of New York includes three first-order streams with very different pH regimes: one moderately acidic (pH 5.8-7.2), the others much more so (pH generally 4.4-5.0)2The less acidic (acidophilic) site yielded a relatively diverse, well balanced fauna, with at least 10% each of midges, mayflies, stoneflies and elmid beetles. Ephemerella funcralis (Ephemeroptera) and Oulimnius latiusculus (Coleoptera) were dominant in this community. They appear to be among the most acid-tolerant species of their respective families and may become abundant under moderately acidic conditions. Their absence or low abundance in the more acidic tributaries of the Silver Run system suggests that they arc eliminated as acidification intensifies, These species therefore may he useful indicators of advancing acidification.3The more acidic (acidobiontic) communities contained fewer Hum half as many taxa as the acidophilic communities, contained few elmid beetles or mayflies, and were heavily dominated In stoneflies. Leuctra feiruinea and Isopecrla sp. were dominant, comprising.56-86% of the individuals. Other common constituents included black flies (Simuliumn gouldingi and S. venustum) and the caddisfly Rhyacvphila fuscula.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2427.1985.tb00241.x
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  • 5
    Electronic Resource
    Electronic Resource
    Prophylactic amrinone for weaning from cardiopulmonary bypass* (2000)
    Oxford, UK : Blackwell Science Ltd
    Anaesthesia 55 (2000), S. 0 
    Details
    ISSN: 1365-2044
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: This prospective, randomised, double-blind, controlled clinical study was performed at a single tertiary referral centre to test the hypothesis that the prophylactic administration of amrinone before separation of a patient from cardiopulmonary bypass decreases the incidence of failure to wean, and to identify those patients who could be predicted to benefit from such pre-emptive management. Two hundred and thirty-four patients, scheduled to undergo elective cardiac surgery, were randomly allocated to receive either a bolus dose of 1.5 mg.kg−1 amrinone over 15 min, followed by an infusion of 10 µg.kg−1.min−1, or a bolus of placebo of equal volume followed by an infusion of placebo. Treatment with amrinone or placebo was initiated upon release of the aortic cross-clamp, before weaning from cardiopulmonary bypass. Anaesthetic technique, monitoring and myocardial preservation methods were standardised for both groups. Significantly fewer patients failed to wean in the group that received prophylactic amrinone than in the control group (7 vs. 21%, p = 0.002). Amrinone improved weaning success regardless of left ventricular ejection fraction, although this benefit was statistically significant only in the group with left ventricular ejection fractions 〉 55%. Of the 32 patients who failed to wean from cardiopulmonary bypass, 14 had normal pre-operative left ventricular ejection fractions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2044.2000.01372.x
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  • 6
    Electronic Resource
    Electronic Resource
    Isolation and characterization of α-aminoadipate-δ-semialdehyde overproducing mutants from yeasts (1989)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 60 (1989), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract We isolated from Candida maltosa mutants lacking saccharopine reductase (lys9) and saccharopine dehydrogenase (lys1). They accumulated α-aminoadipate-δ-semialdehyde (AASA) in the cell and excreted it into the culture medium. In the presence of 15 g glucose/l, 1.25 g NH4H2PO4/l and 50 mg l-lysine/l in a minimal salt medium C. maltosa G285 (lys1) produced about 80–90 mg AASA/l within 48 h. It is the first report of lysine-requiring yeast mutants that accumulate and excrete AASA. In contrast, Pichia guilliermondii lys9 mutants lacked this AASA overproduction. The AASA accumulation by C. maltosa mutants may be explained by the low feedback regulation of their homocitrate synthase and the equilibrium of the enzyme reactions involved in the lysine biosynthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1989.tb03446.x
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  • 7
    Electronic Resource
    Electronic Resource
    Inhibition of tyrosine-sensitive 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase by glyphosate in Candida maltosa (1984)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 23 (1984), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The broad-spectrum herbicide glyphosate (N-[phosphonomethyl]glycine) is a reversible inhibitor of the tyrosine-sensitive 3-deoxy-d-arabino-heptulosonate 7-phosphate (DAHP) synthase from Candida maltosa. Inhibition of the tyrosine-sensitive DAHP synthase reaction by glyphosate is competitive with respect to phosphoenolpyruvate, with Ki 1.3 mM, and noncompetitive with respect to erythrose-4-phosphate. Furthermore, in the presence of 5 mM glyphosate cultured yeast cells excrete shikimic acid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1984.tb01025.x
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  • 8
    Electronic Resource
    Electronic Resource
    Chorismate mutase isoforms from seeds and seedlings of Papaver somniferum
    Amsterdam : Elsevier
    Phytochemistry 31 (1992), S. 2983-2987 
    Details
    ISSN: 0031-9422
    Keywords: Papaver somniferum ; Papaveraceae ; chorismate mutase ; enzyme regulation. ; isoforms
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0031-9422(92)83431-W
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  • 9
    Electronic Resource
    Electronic Resource
    Further identification of Hippeastrum Mosaic Virus (1970)
    Springer
    European journal of plant pathology 76 (1970), S. 171-173 
    Details
    ISSN: 1573-8469
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Samenvatting Infectieus virus kon worden geïsoleerd uitHippeastrum-planten die mozaïekverschijnselen vertoonden, door middel van centrifugeren in een dichtheidsgradiënt. Het materiaal werd bekeken in een elektronenmicroscoop en gefotografeerd. De gemiddelde lengte van de buigzame staafies van hetHippeastrum-mozaïekvirus, berekend uit 600 metingen, bedraagt 643±24 nm.
    Notes: Abstract Infectious virus was isolated fromHippeastrum plants by density gradient centrifugation and examined with the electron microscope. The average lengths of the flexuous particles were 643±24 nm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01974326
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  • 10
    Electronic Resource
    Electronic Resource
    Heterologous gene expression of the glyphosate resistance marker and its application in yeast transformation (1989)
    Springer
    Current genetics 15 (1989), S. 91-98 
    Details
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Vector ; Glyphosate resistance ; Transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The E. coli aroA gene was inserted between yeast promoter and terminator sequences in different shuttle expression plasmids and found to confer enhanced EPSP synthase activity as well as resistance to glyphosate toxicity. Subsequently, a transformation system using these newly constructed vectors in yeast was characterized. The efficiency of the glyphosate resistance marker for transformation and selection with plasmid pHR6/20-1 in S. cerevisiae laboratory strain SHY2 was found to be relatively high when compared with selection for LEU2 prototrophy. The fate of the recombinant plasmid pHR6/20-1 in the transformants, the preservation of the aroA E. coli DNA fragment in yeast, mitotic stability, EPSP synthase activity, and growth on glyphosate-containing medium have been investigated. As this plasmid also allows direct selection for glyphosate resistant transformants on rich media, the glyphosate resistance marker was used for transforming both S. cerevisiae laboratory strain SHY2 and brewer's yeast strains S. cerevisiae var. “uvarum” BHS5 and BHS2. In all cases, the vector pHR6/20-1 was maintained as an autonomously replicating plasmid. The resistance marker is, therefore, suitable for transforming genetically unlabeled S. cerevisiae laboratory, wild, and industrial yeast strains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00435454
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