ISSN:
1365-2109
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
The effects of supplementary shrimp head meal contaminated with white spot syndrome virus (WSSV-SHM) in the diet on detection of WSSV in Penaeus monodon Fabricius were investigated. In Experiment I, 15 shrimp with a mean body weight of 18.2 g were fed to apparent satiation with each of four diets for 8 weeks. Diet 1 was the control diet containing no WSSV-SHM; Diets 2–4 contained wet-cooked WSSV-SHM (autoclaved at 115°C for 15 min), dry-cooked WSSV-SHM (oven-dried at 90°C for 1 h) and commercial SHM at a level of 10% in the diets, respectively. In Experiment II, five diets were used: Diet 1 as the control diet without WSSV-SHM, Diets 2–5 containing steamed WSSV-SHM (100°C for 15 min), oven-dried WSSV-SHM (60°C for 8 h), raw fresh WSSV-SHM and freeze-dried WSSV-SHM at 10% in each diet, respectively. Shrimp, weighing 10.8 g, were fed each diet for 6 weeks to satiation. In both Experiments I and II, the pooled hemolymph samples from five shrimps were taken with 2-week feeding interval and determined in triplicate for WSSV detection using polymerase chain reaction (PCR) assay. In both Experiments I and II, PCR products from hemolymph samples showed the negative results for all dietary treatments. These results suggested that using commercial SHM and WSSV-SHM in diets had no adverse effects on WSSV infection in P. monodon.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1046/j.1355-557x.2001.00029.x
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