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  • 1
    Electronic Resource
    Electronic Resource
    Application of fiber-optic fluorescence measurements to on-line pH monitoring of a pseudomonad fermentation process (1991)
    s.l. : American Chemical Society
    Biotechnology progress 7 (1991), S. 564-569 
    Details
    ISSN: 1520-6033
    Source: ACS Legacy Archives
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bp00012a012
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation, identification, and accumulation of 2-acetamidophenol in liquid cultures of the wheat take-all biocontrol agent Pseudomonas fluorescens 2-79 (2000)
    Springer
    Applied microbiology and biotechnology 54 (2000), S. 376-381 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Pseudomonas fluorescens strain 2-79 (NRRL B-15132) is a classic biological control agent known to produce phenazine-1-carboxylic acid (PCA) as its primary means of suppressing take-all disease of wheat. In addition to PCA, an unknown metabolite was discovered in a liquid culture used to produce the biocontrol agent. The objective of the current study was to isolate, identify, and evaluate the accumulation of this compound in production cultures. Upon centrifugal fractionation of a production culture, thin-layer chromatography (TLC) analyses of extracts of the cells and cell-free supernatant indicated the compound to be primarily in the supernatant. Purified compound was obtained by extraction of culture supernatant, followed by flash chromatography of the extract and preparative TLC. The 1H and 13C nuclear magnetic resonance and electron impact mass spectra indicated the compound to be 2-acetamidophenol (AAP). Measured by reversed-phase HPLC, the accumulations of AAP and PCA in cultures of strain 2-79 reached 0.05 g/l and 1 g/l, respectively. The accumulations of AAP and PCA in liquid cultures were linearly correlated (P 〈 0.001), as shown by studies of cultures stimulated to yield varying levels of PCA by controlling levels of oxygen transfer, pH, and growth medium composition. In this study, oxygen limitation, a defined amino-acid-free medium, and neutral pH stimulated maximal production of both AAP and PCA. Furthermore, a transposon mutant of 2-79 [2A40 2-79 (phz–)] unable to produce PCA did not accumulate AAP. These findings indicate that AAP and PCA are likely to share a common segment of biosynthetic pathway. This is the first report of AAP production by a strain of P. fluorescens. Possible routes of AAP production are discussed relative to current knowledge of the phenazine biosynthetic pathway of strain 2-79. The pertinence of AAP to the design of commercial seed inoculants of phenazine-producing bacteria for controlling wheat take-all is also considered.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530000409
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  • 3
    Electronic Resource
    Electronic Resource
    NUTRITIONAL STABILITY OF CORN-SOY-MILK BLENDS AFTER DRY HEATING TO DESTROY SALMONELLAE (1980)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 45 (1980), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Enriched blends of corn meal, soy flour, and nonfat dry milk (CSM) were adjusted to 7 and 10% moisture and inoculated with approximately 103 cells of Salmonella anatum and Salmonella senftenberg per gram of CSM. The blends were stored at -18 to 60°C and then tested for salmonellae and nutritional changes. S. senftenberg was more heat resistant than S. anatum. At temperatures of 43, 49, 54, and 60°C, S. senftenberg was killed by dry heat after 13, 10, 8, and 6 days, respectively, independent of product moisture. Smaller nutritional losses were observed at 7% than at 10% moisture. Changes in product color, PER values, vitamins A and C, and available lysine were minimal at temperatures of 43 and 49°C for 13 and 10 days, respectively. At 54 and 60°C for 8 and 6 days, nutrient losses were excessive at 10% moisture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1980.tb07491.x
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  • 4
    Electronic Resource
    Electronic Resource
    INFLUENCE OF BACTERIA ON THE CARBONYL COMPOUNDS OF GROUND PORCINE MUSCLE (1973)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 38 (1973), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Quantitative data were obtained for total carbonyls, total monocarbonyls, methyl ketones, saturated aldehydes, 2-enals and 2,4dienals from fresh, reduced surface flora (Dip) and inoculated muscle samples incubated at the optimal growth temperature of the respective bacteria. Individual monocarbonyl compounds were identified by thin-layer and gas liquid chromatography. Micrococcus cryophilus, Pseudomonas fluorescens and Staphylococcus aureus decreased total carbonyls by 57, 18 and 43%, respectively, and total monocarbonyls by 53, 20 and 33%, respectively. Pediococcus cerevisiae increased the total carbonyl and total monocarbonyl content by 70 and 71%. Concentrations of carbonyls in the control samples were directly related to temperature of incubation. Methyl ketones, saturated aldehydes, 2-enals and 2,4dienals were decreased by M. cryophilus, Ps. fluorescens and S. aureus but each monocarbonyl class was increased by P. cerevisiae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1973.tb02779.x
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  • 5
    Electronic Resource
    Electronic Resource
    FUNGAL DETERIORATION OF BAGS DURING INTERNATIONAL TRANSPORT OF CORN SOYA MILK: A TEST SHIPMENT (1979)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 44 (1979), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Losses due to mold deterioration have developed in shipments of dry corn-soya-milk (CSM) made through the U.S. Food for Peace Program. USDA, the dry milling industry and a container manufacturer cooperated in a large-scale test shipment from Milwaukee, WI, to Cochin, India. Conventional multiwall paper bags and two types of polyethylene-coated bags of CSM were randomly stowed in different areas of the ship. During unloading, 13% of all bags had visible mold. Surface fungal counts on both conventional and poly-coated bags had increased a hundredfold during transit. However, mold penetration was slight and contents were unaffected. Mold penetrated and destroyed conventional bags stored under special conditions to induce mold growth, whereas penetration of poly-coated bags was usually associated with a fracture and was less severe. Bag structure, CSM temperature and ventilation were identified as factors influencing mold losses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1979.tb03800.x
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  • 6
    Electronic Resource
    Electronic Resource
    Stoichiometry and Kinetics of Xylose Fermentation by Pichia stipitis (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 589 (1990), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1990.tb24232.x
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  • 7
    Electronic Resource
    Electronic Resource
    INFLUENCE OF CONTROLLED MICROBIAL CURING ON PORCINE MUSCLE (1971)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 36 (1971), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY— A previously developed technique was adopted to study the influence of certain microbiological populations and their effects on processed meat. The technique consisted of an initial reduction of surface bacteria on conventionally handled muscle tissue via a hot water dip, followed by processing at 28° C in a sterile plastic isolator where Pediococcus cerevisiae was introduced into the curing solution. This treatment was compared to reduced initial count and conventional samples. Identification of the bacteria in the curing solution of each treatment indicated that a Lactobacillus spp. was predominant in the reduced initial count treatment. The inoculated Podiococcus cerevisiae was predominant in the reduced initial count inoculated treatment white Staphylococcus epidermidis and Flavobacterium diffusum were predominant in the conventional treatment depending upon the trial. Tenderness and bacterial load were significantly (P 〈 .01) increased by treatment while pH was significantly (P 〈 .01) decreased. Oxidation and muscle composition were not affected by treatment. Samples from all treatments were acceptable organoleptically.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1971.tb06385.x
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  • 8
    Electronic Resource
    Electronic Resource
    Arachidonic-acid production by species of Mortierella (1996)
    Springer
    World journal of microbiology and biotechnology 12 (1996), S. 91-96 
    Details
    ISSN: 1573-0972
    Keywords: Arachidonic acid ; fatty acids ; fungi ; lipids ; Mortierella
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A growth-inhibiting, aspirin-containing medium was developed to select arachidonic-acid-(ARA)-producing Mortierella species and to determine the fatty-acid content of 87 Mortierella strains. ARA was detected in 66 strains from 33 species and its production may prove useful for systematic studies on Mortierella spp. The ARA content of the 66 producing strains tested ranged from 4% to 55% of total lipids. Most of the ARA-producing strains of Mortierella (59 strains) grown on potato/glucose/agar synthesized 〈20% ARA. Four strains produced between 20% and 25% ARA and three produced 〉40%. An inverse relationship was observed between ARA and oleic-acid contents.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00327809
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  • 9
    Electronic Resource
    Electronic Resource
    Pullulan production by color variant strains ofAureobasidium pullulans (1988)
    Springer
    Journal of industrial microbiology and biotechnology 3 (1988), S. 231-239 
    Details
    ISSN: 1476-5535
    Keywords: Aureobasidium pullulans ; Color variant ; Cornstarch ; Pullulan ; Regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Naturally occurring ‘color variant’ strains ofAureobasidium pullulans are distinguished from typical strains by their brilliant pigmentation, overproduction of secreted enzymes (xylanase), and low DNA relatedness. Color variants have not previously been examined for pullulan secretion. Among five independently isolated color variants, strains NRRL Y-12,974 and YB-4026 made the greatest amounts of pullulan from cornstarch, with conversion efficiencies of about 10%. Neither color variant nor typical strains made significant amounts of pullulan from the unconventional lactose or xylan substrates. Pullulan yields were inversely correlated with biomass production. Pullulan production thus appears to be a variable characteristic of both color variant and typically pigmented strains ofA. pullulans, regulated by specific inducers during growth limitation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01569581
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  • 10
    Electronic Resource
    Electronic Resource
    Ethanol production from hemicellulose hydrolysates of agricultural residues using genetically engineeredEscherichia coli strain KO11 (1996)
    Springer
    Journal of industrial microbiology and biotechnology 16 (1996), S. 42-47 
    Details
    ISSN: 1476-5535
    Keywords: ethanol ; E. coli ; biomass ; lignocellulose ; pentose ; hemicellulose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Hemicellulose hydrolysates of the agricultural residues bagasse, corn stover, and corn hulls plus fibers were readily fermented to ethanol by recombinantEscherichia coli strain KO11. Corn steep liquor and crude yeast autolysate served as excellent nutrients. Fermentations were substantially complete within 48 h, often achieving over 40 g ethanol L−1. Ethanol yields ranged from 86% to over 100% of the maximum theoretical yield (0.51 g ethanol g sugar−1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01569920
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