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  • 1
    Electronic Resource
    Electronic Resource
    The activity of a single-stranded promoter of plasmid ColIb-P9 depends on its secondary structure (2004)
    Oxford, UK : Blackwell Science Ltd
    Molecular microbiology 53 (2004), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The leading region of the conjugal bacterial plasmid ColIb-P9 contains three dispersed repeats of a 328 bp sequence homologous to Frpo, a sequence from plasmid F that acts as a promoter in single-stranded DNA. One of these sequences, ssi3, inactive in the double-stranded form, promoted in vitro transcription exclusively from the single strand that is transferred during conjugation. Promoter activity was dependent on the presence of RNA polymerase holoenzyme containing sigma 70. Transcription initiated from the position predicted from folding the single-stranded DNA to form a pseudo double-stranded hairpin structure containing recognizable −35 and −10 promoter elements. Footprinting of RNA polymerase holoenzyme on single-stranded ssi3 DNA was consistent with this suggestion. Mutagenesis of the putative −35 region inactivated the promoter, but random mutations in the −10 region had little effect. The putative −10 region is a poor match to the consensus sequence and contains mismatched bases. Elimination of these mismatches invariably destroyed single-strand promoter activity. These observations reveal the crucial contribution of the unpaired bases in the −10 region in potentiating the formation of the productive open complex with RNA polymerase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.2004.04114.x
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  • 2
    Electronic Resource
    Electronic Resource
    Regulation of Bacterial Enzyme Synthesis by Induction and Repression (1964)
    [s.l.] : Nature Publishing Group
    Nature 203 (1964), S. 1153-1155 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] ENZYME synthesis in bacteria can be induced or repressed by changes in the external environment. The essential similarity of these two processes was explicitly recognized by Jacob and Monod1 when they put forward the repressor-operator model for the regulation of enzyme synthesis. In general, ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/2031153a0
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  • 3
    Electronic Resource
    Electronic Resource
    Out of sequence (1987)
    [s.l.] : Nature Publishing Group
    Nature 330 (1987), S. 432-432 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] FOR obvious reasons, much of the exciting progress in modern biology has centred on 'the gene'. New insights into gene structure and function have been frequent and often surprising in the past decade. The potential for direct application of molecular genetics in biotechnology and its importance ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/330432b0
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  • 4
    Electronic Resource
    Electronic Resource
    Characterisation of Kir2.0 proteins in the rat cerebellum and hippocampus by polyclonal antibodies (1999)
    Springer
    Histochemistry and cell biology 112 (1999), S. 457-465 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  Rabbit polyclonal antibodies were raised to rat Kir2.0 (Kir2.1, Kir2.2 and Kir2.3) inwardly rectifying potassium ion channel proteins. The antibody specificities were confirmed by immunoprecipitation of [35S]-methionine-labelled in vitro translated channel proteins and western blotting. Immunohistochemistry revealed a different patterns of expression of Kir2.0 subfamily proteins in the rat hind-brain (cerebellum and medulla) and fore-brain (hippocampus). Notably, only Kir2.2 protein was detected in the cerebellum and medulla, Kir2.1, Kir2.2 and Kir2.3 proteins were expressed in the hippocampus and immunostaining was not limited to neuronal cell types. Anti-Kir2.1 (fore-brain only) and anti-Kir2.2 (fore- and hind-brain) antibodies showed positive staining in macroglia, endothelia, ependyma and vascular smooth muscle cells. In contrast, anti-Kir2.3 (fore-brain only) immunostaining was limited to neurons, macroglia and vascular smooth muscle. These results indicate that specific regions within the rat fore- and hind-brain have differential distributions of inwardly rectifying potassium ion channel proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004180050429
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  • 5
    Electronic Resource
    Electronic Resource
    Alignment of clonedamiE gene ofPseudomonas aeruginosa with theN-terminal sequence of amidase (1981)
    Springer
    Bioscience reports 1 (1981), S. 299-307 
    Details
    ISSN: 1573-4935
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01114869
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  • 6
    Electronic Resource
    Electronic Resource
    The pro-peptide is not necessary for active renin secretion from transfected mammalian cells (1989)
    New York, NY : Wiley-Blackwell
    Proteins: Structure, Function, and Genetics 5 (1989), S. 259-265 
    Details
    ISSN: 0887-3585
    Keywords: cDNA expression ; deletion mutagenesis ; zymogen activation ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Cultured mouse myeloma cells were transfected with expression vectors encoding the aspartyl proteinase, human renin. The full construct, encoding the renin precursor prorenin, allows transfected cellsto secrete the enzymically inactive pro-protein. Activity is detectable only following trypsin treatment which mimics the physiological activation step. Accordingly, it appears that myeloma cells do not contain detectable levels of an appropriate activating proteinase. However, when these cells are transfected with a construct from which the pro-peptide coding sequence has been deleted, they secrete an apparently fully active enzyme which is indistinguishable from mature renin. We conclude that expression of the pro-peptide is not necessary to allow correct folding of the molecule and its passage through the secretory pathway.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/prot.340050402
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  • 7
    Electronic Resource
    Electronic Resource
    The Cis-specificity of the Q-gene product of bacteriophage lambda (1982)
    Springer
    Molecular genetics and genomics 185 (1982), S. 468-472 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A rtrp/lac W205 substitution, fused to the late ragion of bacteriophage lambda, provided a convenient assay for phage late gene expression in the presence or absence of lambda pQ. Comparison of lacZ expression from Q + and Q - phages showed that late gene expression was markedly Q-dependent (263-fold difference). A cis/trans comparison of lambda pQ action showed a 180-fold difference in lacZ expression. The results suggest that pQ is only significantly active when supplied in cis to its site of action.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00334142
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  • 8
    Electronic Resource
    Electronic Resource
    Transcriptional termination sites in the b2 region of bacteriophage lambda that are unresponsive to antitermination (1982)
    Springer
    Molecular genetics and genomics 185 (1982), S. 462-467 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A bacteriophage lambda cloning vector carrying the trp/lacW205 substitution is described. The vector facilitates the fusion in vitro of genetic control signals to the lacZ structural genes of Escherichia coli. This system was used to define transcriptional termination sites in the lambda b2 region. This region contains termination sites that are unresponsive to the λ antiterminating proteins pQ and pN.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00334141
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  • 9
    Electronic Resource
    Electronic Resource
    The construction in vitro of transducing derivatives of phage lambda (1976)
    Springer
    Molecular genetics and genomics 146 (1976), S. 199-207 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Methods are described for the construction of plaque-forming, transducing derivatives of phage lambda, using appropriate receptor genomes and fragments of DNA generated by the restriction enzymes endo R.EcoRI and endo R.HindIII. The general properties of the transducing derivatives are described and discussed. Plaque-forming phages carrying the E. coli trp, his, cysB, thyA, supD, supE, supF, hsd, tna and lig genes have been isolated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268089
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  • 10
    Electronic Resource
    Electronic Resource
    The use of specialised transducing phages in the amplification of enzyme production (1976)
    Springer
    Molecular genetics and genomics 149 (1976), S. 87-99 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two types of λtrp phages have been used as model systems to investigate ways of optimising the expression of bacterial genes from transducing phage genomes. Excellent yields of trp enzymes were achieved by infecting a trpR− host with Q − or Q − Q − S − derivatives of λtrpAM1, which expresses its trp genese exclusively from the trp promoter. The five trp geneproducts constituted more than 50% of the total soluble protein of infected cells under these conditions, and an even higher proportion of the protein synthesized after infection. In a trpR + host, phage DNA replication was easily able to override tryptophan-mediated repression by titration of the trp repressor protein. N − derivatives of λtrp phages carrying the trp promoter were equally productive, while having the advantage of being much simpler to construct and propagate. λtrp phages lacking the trp promoter were used to investigate ways of optimising gene expression initiated at the phage promoter, PL. Though very powerful, the latter promoter is more difficult to harness then the trp promoter. Derepression of transcription from PL by the use of cro − mutations is accompanied by poor replication of transducing phage DNA. Attempts to circumvent this difficulty using virulent of cro, cll double mutants have not been successful. Nevertheless, cells infected with a λtrp phage expressing its trp genes exclusively from PL made up to 16 per cent of their protein as trp gene-products.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00275963
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