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  • 1
    Electronic Resource
    Electronic Resource
    Protoplast production in Chondrus crispus gametophytes (gigartinales, rhodophyta) (1990)
    Springer
    Plant cell reports 8 (1990), S. 582-585 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Protoplasts were isolated from female gametophytes of Chondrus crispus (Stackh.) using commercial cellulase and various carrageenases prepared from marine bacteria. Depending on the nature of the donor tissue (apices or whole thallus, wild or cultivated strains), yields ranged from 1.0–8.5×108 protoplasts per gram of fresh tissue. Preincubating the tissue with a potassium chelator, Kryptofix 222, enhanced protoplast yields by 30–50 %. Based on staining with fluorescein diacetate most protoplasts were viable. A few protoplasts regenerated a cell wall and divided.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00270058
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Preliminary approach to the characterization and seasonal variation of carrageenans from four Rhodophyceae on the Normandy coast (France) (1990)
    Springer
    Hydrobiologia 204-205 (1990), S. 539-544 
    Details
    ISSN: 1573-5117
    Keywords: Cystocloniaceae ; iota-carrageenan ; IR spectra ; Phyllophoraceae ; Rhodophyceae ; seaweed ; tank culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Carrageenans extracted under alkaline conditions were studied in some Rhodophyceae from the Normandy coast. Among these, four species yielding iota-carrageenan were studied throughout a whole year: Calliblepharis ciliata, Calliblepharis jubata, Cystoclonium purpureum and Gymnogongrus crenulatus. Carrageenan content varied with season, being maximal at the end of spring and minimal in autumn, and was positively correlated with the growth of these algae. A culture of Cystoclonium purpureum was initiated and, without trying to optimize growth conditions, yielded a mean production of 50 g fresh wt m−2 d−1 in 36 weeks of continuous tank culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040283
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Carbonic system-incident energy relationship in Chondrus crispus (Rhodophyta, Gigartinales) tank culture (1984)
    Springer
    Hydrobiologia 116-117 (1984), S. 463-466 
    Details
    ISSN: 1573-5117
    Keywords: seaweed ; Chondrus ; tank culture ; carbon absorption ; energy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00027723
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Ammonium uptake by Chondrus crispus Stackhouse (Gigartinales, Rhodophyta) in culture (1990)
    Springer
    Hydrobiologia 204-205 (1990), S. 467-471 
    Details
    ISSN: 1573-5117
    Keywords: Chondrus crispus ; culture ; N-NH4 uptake ; Rhodophyta ; seaweed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cultivated Chondrus crispus was used in N-NH4 uptake experiments in the laboratory. An elevation of temperature increased the apparent rate of uptake, especially up to 11 °C. Uptake in the dark was found to be 83 % of that in the light. The apparent uptake decreased with increasing internal N pool; rates were 26.5, 22.2 and 20.2 µg N g dry wt−1 min−1 for internal N pools of 2.7, 3.5 and 4.6%, respectively. Apparent uptake increased with the substrate N concentration. The resulting curve has two components: an active uptake and a diffusion component at high (〉 5000 µg N L−1) external N levels. Ks and V max were calculated by deducting the diffusion component from the uptake curve: these were of 497 µg N L −1 and 14.4 µg N g dry wt−1 min−1. respectively, and reflect a low substrate affinity. This could be the result of 10 years of continuous culture of C. crispus. Uptake was similarly followed in the culture tanks and showed comparable results; nighttime would be the most appropriate time to supply nutrients.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040272
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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