Library

feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 238 (1974), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 238 (1974), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 460 (1985), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 13 (1973), S. 53-62 
    ISSN: 1432-0827
    Keywords: Bioelectric potentials ; Bone ; Negativity ; Cells ; viability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé Des potentiels électriques sont enregistrés au niveau du tibia, au repos, d'un lapin anesthésié, et cela à l'aide d'électrodes d'argent/chlorure d'argent, reliées à des ponts salins et un voltmètre à haute impédance. Les potentiels enregistrés à partir des surfaces antérieures, médianes et latérales du tibia indiquent que le voltage de surface est constant autour de l'axe transversale de l'os, mais varie en rapport avec l'axe longitudinal. Les voltages sont indépendants des limites osseuses et points d'attaches musculaires. Ces potentiels bioélectriques varient peu après résection du nerf sciatique ou ligature de l'artère fémorale. Les potentiels décroissent nettement trente minutes après administration intra-artérielle de dinitrophénol ou iodoacétamide. Les potentiels chutent nettement dans un segment localisé du tibia, endommagé par application d'ultra-sons. La mort de l'animal provoque une chute lente de potentiel en rapport avec la perte graduelle de viabilité des cellules osseuses. Il semble que les potentiels de repos, observés à la surface de l'os non stressé, ne sont pas directement liés à la circulation sanguine ou la transmission neurale. Ces potentiels semblent plutôt liés à l'existence d'une population cellulaire osseuse active et chaque potentiel mesuré constitue la somme des forces électromotrices de toutes les cellules d'une région localisée.
    Abstract: Zusammenfassung Am anaesthesierten Kaninchen wurden die Biopotentiale der unbelasteten Tibia gemessen. Dazu wurden Silber/Silberchlorid-Elektroden benützt, welche über Salzbrücken an ein Voltmeter mit hohem Eingangswiderstand angeschlossen waren. Die an der vorderen, seitlichen und hinteren Tibia-Oberfläche gemessenen Potentiale zeigten, daß die Oberflächenspannung an der Querachse eines Knochens konstant ist, jedoch in bezug zur Längsachse variiert. Die Spannungen waren nicht beeinflußt durch knochentopographische und Muskelansatz-Punkte. Nach Sciaticus-Durchtrennung oder Ligatur der Arterie femoralis änderten sich die bioelektrischen Potentiale nur wenig. Ein eindrücklicher Abfall der Potentiale wurde jedoch 30 min nach intraarterieller Verabreichung von Dinitrophenol oder Jodacetamied beobachtet. Ebenso stark fielen die Potentiale in einem umgrenzten Tibia-Segment ab, das durch Ultraschallbehandlung lädiert worden war. Beim Tod der Tiere wurde ein langsamer Abfall der Potentiale, entsprechend dem allmählichen Verlust an lebenden Knochenzellen, beobachtet. Auf Grund der mit dieser Studie gewonnenen Resultate kann vermutet werden, daß die im Gleichgewicht bleibenden Potentiale der unbelasteten Knochenoberfläche nicht direkt von der Durchblutung oder der nervösen Reizleitung abhängig sind. Es scheint eher, daß dafür eine intakte Knochenzell-Population vorliegen muß, wobei jedes aufgezeichnete Potential eine Summierung der elektromotorischen Kräfte aller Zellen einer bestimmten Region darstellt.
    Notes: Abstract Bioelectric potentials were recorded from the unstressed tibia in the anesthetized rabbit by means of silver/silver chloride electrodes connected to saline bridges and a high impedence voltmeter. Potentials recorded from the anterior, medial, and lateral surfaces of the tibia indicated that the surface voltage was constant around the transverse axis of a bone but varied in relation to its long axis. Voltages were independent of bony landmarks and points of muscle attachment. The bioelectric potentials changed little after sciatic nerve denervation or ligation of the femoral artery. The potentials decreased dramatically thirty minutes following the intra-arterial administration of dinitrophenol or iodoacetamide. Also, the potentials fell sharply in a localized segment of tibia injured through the application of ultrasound energy. Animal death brought about a slow decay of potential commensurate with the gradual loss of bone cell viability. The experimental evidence from this study suggests that the steady state potentials observed from the surface of unstressed bone are not directly dependent on blood flow or nerve transmission. Rather, such potentials are dependent on a functioning bone cell population, and each recorded potential represents a summation of the electromotive forces from all cells in a localized area.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 5
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 29 (1995), S. 1101-1110 
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: The biosynthetic and migratory response of bone cells to changes in both surface composition and morphology of polystyrene (PS) substrates was examined. A system was devised wherein micromachined silicon wafers were used as templates to solvent-cast PS replicas [using 0, 1, or 2 wt % styrene (S) monomer additions] with either 0.5- or 5.0- μm-deep surface grooves. Smooth replicas (0% S) served as the control surfaces. The chemical and morphologic characteristics of the nine unique model biomaterial surfaces (MBSs) produced using this system were documented and were found to be distinct. For the biosynthetic studies, bone cells isolated from neonatal rat calvaria were plated onto the MBSs and labeled at postconfluence with [14C]proline for 24 h. Total DNA per surface, total newly synthesized collagenous (CP), and noncollagenous protein (NCP) (cell associated and secreted) were determined. Cell-associated CP was found to increase significantly for the bone cells cultured on the substrates with 0.5-μm grooves and 2% S (P 〈 .05). Cell-associated NCP was found to be elevated for all 2% S substrates and for the 0.5-μm grooves substrates with 1% S. For the migration studies, bone cells were plated first onto 5-mm nitrocellulose disks that were attached to standard Petri dishes using a plasma clot. At confluence, the disks were removed aseptically and placed on the replicas. The cellular area occupied as a result of the outward migration of the bone cells was measured after 4 days of culture using an image analysis system. An average velocity for the leading edge of bone cell populations on each of the nine MBSs was calculated: Cells on surfaces with either 1% S or 5.0-μm grooves displayed significantly higher velocities than did the control cultures. A significant interaction effect between chemistry and morphology was observed. The biosynthetic and migratory responses of in vitro cultures of bone cells were not predictable from the observations of the cellular responses to the individual features, but appeared to depend on cellular responses to more than one substrate factor. © 1995 John Wiley & Sons, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 6
    Electronic Resource
    Electronic Resource
    Hoboken, NJ [u.a.] : Wiley-Blackwell
    Journal of Orthopaedic Research 3 (1985), S. 125-136 
    ISSN: 0736-0266
    Keywords: Electrical stimulation ; Osteogenesis ; Platinum ; Stainless steel ; Electrode materials ; Microtrauma ; Life and Medical Sciences
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Studies of electrical stimulation of osteogenesis with stainless steel electrodes have previously established a dose-response relationship between current and bone growth. Examination of the effect of different geometric current densities resulted in the conclusion that very little electrode surface area was involved in stimulation and led to the design of a multiport “distributive” cathode. A series of experiments were performed to extend these results to wire and multiport platinum electrodes. As before, a current-bone growth dose-response relationship was found. Peak bone growth was greater than for stainless steel. However, peak bone growth occurred at 2.0 μA (versus 20 μA for stainless steel). Correlation studies suggest that small changes in cathodic potential affect bone growth more than similar size changes in current. Finally, the generally benign local host response to platinum suggests that platinum may be a suitable materials for chronic indwelling anodes for stimulation of osteogenesis.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 7
    Electronic Resource
    Electronic Resource
    Hoboken, NJ [u.a.] : Wiley-Blackwell
    Journal of Orthopaedic Research 2 (1984), S. 235-246 
    ISSN: 0736-0266
    Keywords: Growth plate chondrocyte ; Mitochondrial isolation ; Chondrocyte metabolism ; Cytochrome aa3 ; Oxygen consumption ; Life and Medical Sciences
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The growth plate chondrocyte plays a central role in growth plate function. The purpose of this study was to characterize the respiratory and calcium transport properties of isolated mammalian growth plate chondrocytes and mitochondria obtained from these cells and to quantitate the mitochondrial weight and volume fraction in each zone of the growth plate. A new method was developed for isolation of mitochondria from chondrocyte suspensions. Isolated chondrocyte mitochondria demonstrated an eightfold increase in oxygen consumption in response to calcium and a two- to threefold increase in oxygen consumption in response to adenosine diphosphate. Similar responses were observed in chondrocytes treated with digitonin. The mitochondrial protein content of the growth plate and hyaline cartilage chondrocytes is significantly less than hepatocytes. Conversely, the chondrocyte mitochondrial cytochrome aa3 content is similar to mitochondria from a wide variety of sources. A zonal analysis of the growth plate demonstrates an increase in the mitochondrial weight (protein) fraction from the reserve to the hypertrophic zone whereas the mitochondrial volume fraction decreases from the reserve to the hypertrophic zone. The findings of this study emphasize the dependence of chondrocytes on glycolysis as a prime energy source and support the concept that chondrocyte mitochondria have become specialized in the process of matrix calcification.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 8
    Electronic Resource
    Electronic Resource
    Hoboken, NJ [u.a.] : Wiley-Blackwell
    Journal of Orthopaedic Research 3 (1985), S. 137-139 
    ISSN: 0736-0266
    Keywords: Electrical osteogenesis ; Direct current ; Stainless steel electrodes ; Medulary canal ; Life and Medical Sciences
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Reactive, post-traumatic bone formation in response to intramedullary insertion of a polytetrafluorethylene-coated, 28 gauge stainless steel wire was compared with the sequential bone formation seen in response to an indentical intramedullary stainless steel cathode delivering 20 μA constant direct current. Animals were studied at days 1, 2, 3, 4, 5, 7, 9, 11, 14, 17, and 21 after wire insertion. Point count analysis revealed progressive bone formation beginning as early as day 3 on the constant direct current-stimulated side, progressing steadily through day 21. Control tibia, however, began to show bone formation on day 5 with peaking at day 9 and subsequent bone resorption. The osteogenic response at the 20 μA cathode was statistically elevated above that seen at the control on days 11, 17, and 21.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 9
    Electronic Resource
    Electronic Resource
    Hoboken, NJ [u.a.] : Wiley-Blackwell
    Journal of Orthopaedic Research 7 (1989), S. 511-518 
    ISSN: 0736-0266
    Keywords: Intracellular calcium ; Growth Plate ; Mineralization ; Fura 2 ; Calcium loading ; Life and Medical Sciences
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: In isolated chondrocytes from the growth plate, there is an increase in both the cytosolic ionized calcium concentration and in total cell calcium content as the cells approach the mineralization front. The reserve zone chondrocytes have a cytosolic ionized calcium concentration, [Ca2+]i, of 124 ± 23 nM and a total cell calcium content, CaT, of 12.8 ± 6.8 nmol/mg cell protein. Proliferative zone chondrocytes have a [Ca2+]i of 172 ± 29 nM and a CaT of 16.1 ± 11.8 nmol/mg cell protein. Hypertrophic zone chondrocytes have a [Ca2+]i of 273 ± 49 nM and a CaT of 35.8 ± 16 nmol/mg protein. Chondrocytes isolated from the entire growth plate have a [Ca2+]i of 132 ± 29 nM and a CaT of 13.2 ± 2.5 nmol/mg cell protein. Chondrocytes isolated from noncalcifying hyaline cartilage have a [Ca2+]i of 85 ± 10 nM and a CaT of 11.5 ± 1.4 nmol/mg cell protein. There appears to be a correlation between intracellular calcium accumulation, an increase in the intracellular ionized calcium concentration, and the process of cartilage matrix mineralization.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 10
    Electronic Resource
    Electronic Resource
    Hoboken, NJ [u.a.] : Wiley-Blackwell
    Journal of Orthopaedic Research 6 (1988), S. 239-247 
    ISSN: 0736-0266
    Keywords: Pulsing electromagnetic fields ; Osteogenic tissue ; Costochondral junction ; Life and Medical Sciences
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Specific pulsing electromagnetic fields (PEMFs) have been used to stimulate growth and repair of osteogenic tissues; however, the basis for this specificity is unknown. Previously, we determined the relevant electromagnetic field parameters of the clinically used PEMF and independently verified the beneficial effects of PEMFs on the rabbit fibula fracture healing model. The goal of the present study was to develop an in vitro model that would permit the effectiveness of various electric and magnetic field components of the PEMF to be determined.The costochondral junction (CCJ) of the 21-day-old rat was exposed in vitro to PEMFs with various electric and magnetic field component amplitudes. Response of this model to PEMFs was determined by nondestructive macrophotographic measurement of CCJ growth. Preliminary data indicated that temperature effects were present in this in vitro system. Subsequent experiments designed to separate the effects of temperature and PEMFs on the growth of CCJs in tissue culture were performed.Results indicate that accurate and frequent temperature measurements must be made for in vitro models being used to study effects of PEMFs. Small temperature differences induced by the coils used to produce PEMFs in the CCJ experimental system can have significant stimulatory effects, and the combined effects of temperature and PEMFs are not linearly additive in this model. Furthermore, our results suggest that thermal and PEMF stimuli could affect macrophotographically measured growth of the CCJ by separate mechanisms or could have a synergistic effect. Therefore, PEMF stimulation experiments should be performed under strictly “athermal” conditions.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...