ISSN:
1573-7373
Keywords:
lectin histochemistry
;
cell proliferation
;
astrocytic tumor
;
human
;
in vitro
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The role of lectins as biosignalling molecules oras markers of human astrocytic tumors remains relativelyunexplored. The aim of the present work isto investigate (1) whether or not human astrocytictumors express specific glycans, evidenced experimentally by meansof lectin histochemistry, and (2) whether, in turn,these lectins can significantly modulate astrocytic tumor cellproliferation. Using a cell image processor, we thereforebegan by quantitatively measuring the histochemical binding patternof 5 lectins (WGA, PNA, PHA-L, GSA-IA4 andCon A) in 5 astrocytomas, 5 anaplastic astrocytomasand 5 glioblastomas. Secondly, we measured the influenceof these 5 lectins on the proliferation of3 astrocytic tumor cell lines (SW1088, U373 andU87) growing in vitro as monolayers. Cell proliferationwas assessed by means of the colorimetric MTTassay. The histochemical lectin staining markedly varied intra-and inter-group. However, some constant results were obtained.Indeed, the staining increased markedly from GSA-IA4 andPHA-L through WGA and PNA to Con Ain the three histopathological groups. The assessment ofcell proliferation demonstrated that WGA, Con A andPHA-L very significantly decreased proliferation in the 3astrocytic cell lines in a dose-dependent manner. Astrocytictumor cells in the confluent growth phase wereless sensitive to the WGA, Con A andPHA-L lectin-induced effects than cells in the loggrowth phase. The GSA-IA4 and PNA lectins hadglobally very weak effects on the proliferation ofthe astrocytic tumor cell lines. Increasing the fetalcalf serum from 1% to 10% in theculture media significantly antagonized the WGA-, Con A-and PHA-L-induced cell proliferation decrease in the 3astrocytic cell lines. In conclusion, the present datastrongly suggest that some lectins (including WGA, ConA and PHA-L) significantly influence the proliferation ofastrocytic tumor cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1005783321916
Permalink
