ISSN:
1432-0797
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract Saccharomyces cerevisiae was grown in aerobic continuous culture on a defined minimal medium, with glucose (40 g.l−1) as the growth-limiting carbon source, to acquire knowledge useful in process design and for model-based control. Steady-state concentrations of biomass, glucose, ethanol and activities of model products alcohol dehydrogenase, hexokinase, malate dehydrogenase, glucose-6-phosphate dehydrogenase and iso-citrate dehydrogenase were determined at dilution rates (D) between 0.06 h−1 and 0.323 h−1 (close to μmax). Enzyme activities showed productivity trends related to the transition from oxidative to oxido-reductive growth. Conclusions are drawn from the data with regard to designing a new process for production of intracellular enzymes. Issues of process stability as well as productivity are discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02391584
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