Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Characterizing and sexing laminarialean meiospores by flow cytometry (1989)
    Springer
    Marine biology 101 (1989), S. 451-456 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Selected features of Alaria marginata Postels et Ruprecht, Laminaria saccharina (L.) Lamouroux and Cymathere triplicata (Postels et Ruprecht) J. G. Agardh meiospores were described using flow cytometry. The relative sizes (forward scatter), chlorophyll contents (red fluorescence) and DNA contents (blue fluorescence) were measured on living, fixed, Hoechst and DAPI (4′,6-diamidino-2-phenylindole) stained and unstained cells. The meiospores were similar among species and the frequency distributions of the monitored features were essentially normal. Meiospores sorted for low blue fluorescence departed significantly from the expected 1:1 male to female ratio in favour of the male. Cells sorted for high blue fluorescence did not result in a departure from the expected 1:1 ratio. We suggest that our ability to sexually sort meiospores is a result of a differential nonstoichiometric fluorescence in DNA, which possibly reflects a greater DNA compactedness and/or nuclear protein content in the male meiospores.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00541646
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Book review (1981)
    Springer
    Microbial ecology 7 (1981), S. 95-95 
    Details
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02010481
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    On describing microbial growth kinetics from continuous culture data: Some general considerations, observations, and concepts (1975)
    Springer
    Microbial ecology 2 (1975), S. 261-283 
    Details
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Analysis of continuous culture methodology suggests that this potentially powerful tool for kinetic analysis can be improved by minimizing several inherent shortcomings. Medium background substrates — organic carbon, phosphate, and manganese — were shown to dominate kinetic observations at concentrations below chemical detection methods. Reactor wall growth, culture size distribution changes, sample removal-induced steady state perturbations, and limiting substrate leakage from organisms are treated in terms of kinetic measurement errors. Large variations in maximal growth rates and substrate uptake rates found are attributed to experimental protocol-induced transient states. Relationships are presented for correcting limiting substrate concentrations for lability during sampling, contamination with unreacted medium, and background substrate effects. Analytical procedures are discussed for improved measurement of limiting substrate kinetics involving enzymes, isotopes, and material balance manipulation. Relaxation methods as applied to continuous culture are introduced as a means for isolating separate rate constants describing net substrate transport and for evaluating cellular metabolite leakage. Low velocity growth, multiple substrate metabolism, and endogenous metabolism are discussed along with measurements showing that 1-month generation times for aquatic microorganisms can be quite normal and that the kinetics are compatible withμg/liter limiting substrate concentrations. The concept of regarding growth kinetics as the sum of several net accumulation processes is suggested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02011647
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Reversible bioconcentration of monochlorobiphenyls byRhodotorula rubra: Correlations with aqueous solubility of substrate (1979)
    Springer
    Bulletin of environmental contamination and toxicology 23 (1979), S. 44-50 
    Details
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01769914
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Nutrient-limited microbial growth kinetics: overview and recent advances (1993)
    Springer
    Antonie van Leeuwenhoek 63 (1993), S. 225-235 
    Details
    ISSN: 1572-9699
    Keywords: kinetics ; transport ; collision frequency ; growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Traditional concepts of nutrient uptake and growth kinetics as linked by cell yield are presented. Phenomena affecting the kinetics are examined along with a discussion of those which lead to ambiguity. Concepts of flux control are presented to help understand the distribution of material along metabolic pathways. Specific affinity is described to relate nutrient accumulation rates to transporter density. It is shown to be a primary kinetic constant and the best available index of nutrient collection ability. As an aid to understanding, specific affinity is reexpressed in terms of membrane permeability. Formulations of nutrient transport rate as a function of cellular composition, particularly transporter and enzyme content and known as janusian kinetics, are described as an improvement to specific affinity theory. Procedures for quantified unidirectional fluxes are reviewed to identify the difference between gross and net transport rates of substrate. Collision frequency theory is used to show that in addition to total biomass, cell size and transporter density should also be included in rate equations describing microbial growth. Theory diversity suggests that one reason for microbial metabolic is that the likelihood of additional collisions of substrate molecules with a cell surface, after an initial collision, requires only a sparse distribution of transporter sites for maximal rate, leaving room for additional transporters able to collect other substrate types.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00871220
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...