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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Macromolecules 25 (1992), S. 3309-3312 
    ISSN: 1520-5835
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 83 (1985), S. 3621-3625 
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: The second virial coefficient of 3He gas has been determined between 1.3 and 0.6 K from measurements of pressure and density on several isotherms. The density was derived from the resonance of a superconducting microwave cavity whose frequency was dependent on the dielectric constant of the gas within it. The pressure was also determined using a resonant superconducting rf cavity, in this case the flexing of a diaphragm being the source of the dependence of frequency on pressure. The experimental techniques work well at low pressure and densities. The virial coefficient appears well behaved and in agreement with the experimental work of others at higher temperatures.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Journal of industrial microbiology and biotechnology 22 (1999), S. 71-77 
    ISSN: 1476-5535
    Keywords: Keywords: Fusarium moniliforme; cutinase; polycaprolactone depolymerase; acid protease; biodegradable polymer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Cultures of Fusarium moniliforme grown on polycaprolactone (PCL) or on cutin as a sole source of carbon and energy had low levels of detectable PCL depolymerase (cutinase) activity in the supernatant medium. A small peak of depolymerase activity was observed after hyphal accumulation had ceased, but this activity soon declined. The low level of the peak of activity and its decline were attributable to proteolytic inactivation of the depolymerase. A decrease in the pH of cultures coincided with the appearance of protease activity in the supernatant at about the same time as the appearance of the transient peak of depolymerase activity. Addition of protease substrates (bovine serum albumin, casein) to the culture at this time caused a dramatic although temporary increase in PCL depolymerase activity. The same effect was seen for cultures of F. solani pisi. Use of a different buffer system for the medium prevented a drop in pH and resulted in higher and stable levels of PCL depolymerase activity.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 50 (1998), S. 692-696 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Polycaprolactone (PCL), a synthetic polyester with applications in biodegradable plastics, is degraded by a variety of microorganisms, including fungal phytopathogens. These pathogens secrete cutinase, which hydrolyzes cutin, the polyester structural component of plant cuticle, releasing ω-hydroxy fatty acids that induce cutinase synthesis. Our laboratory previously reported that growth of Fusarium solani on PCL requires cutinase, which is active as a PCL depolymerase and induced by the products of its action on PCL. A mutant strain of F. solani in which the cutinase gene is deleted was unable to grow on PCL and did not secrete PCL depolymerase activity in the media tested. It is now shown that this mutant produces a PCL depolymerase in media containing lipase inducers. Wild-type strains also produce this second PCL depolymerase, which is induced by Tween 80 and tributyrin, but not by PCL or cutin. The second depolymerase shows interfacial activation, indicating that it is a lipase. PCL may thus be a substrate but not an inducer of depolymerases that degrade it, and screening microorganisms on medium with PCL as the sole source of carbon and energy may fail to reveal strains with active PCL depolymerases, because of the absence of an inducer. Surprisingly, Tween 80 induces both cutinase and lipase activities in wild-type F. solani.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Radiation and environmental biophysics 13 (1976), S. 263-266 
    ISSN: 1432-2099
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract Selective X-ray absorption method has been worked out to determine concentration of heavy elements in organic substances. The method is applicable
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract. High-spin states have been studied in 72Kr and 72Br using the 40 Ca + 40 Ca and 36 Ar + 40 Ca reactions at 164 and 145 MeV, respectively. The properties and configurations of the high-spin bands observed have been interpreted using unpaired cranked Nilsson-Strutinsky (CNS), and for 72Kr, paired cranked relativistic Hartree-Bogoliubov (CRHB) calculations. In 72Kr a new band has been identified that has the properties expected for the doubly aligned S-band configuration. In 72Br the previously known bands have been extended to higher spin. This has lead to a re-interpretation of the configurations.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Hyperfine interactions 3 (1977), S. 365-369 
    ISSN: 1572-9540
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The effect of irradiation on WC and WO3 has been observed using perturbed angular correlation techniques. The electric quadrupole attenuation in the angular distribution of γ-rays from the doubly even tungsten nuclei following Coulomb excitation by 6.0 MeV4He+ has been measured. The observed electric field gradients are larger than the fields previously determined following Coulomb excitation using the Mössbauer effect.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 28 (1983), S. 327-334 
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Three high molecular weight polycaprolactones (M̄w = 35,000, 18,600, and 7,130) were utilized as the sole carbon source by five of six fungi tested by the American Standards for Testing and Materials (ASTM) agar plate method. The fungi were Aspergillus flavus, A. niger, A. fumigatus, Chaetomium globosum, Pencillium funiculosum, and a Fusarium sp. Quantitative analysis of degradation was performed using gel permeation chromatography (GPC). GPC analysis demonstrated differences between the activities of organisms which appeared similar by the ASTM method, and showed that, while all molecular weight species within each polymer were hydrolyzed, in several cases low molecular weight end products were not assimilated. Depending on the organism, the dominant factor determining degradability was either polymer molecular weight or degree of crystallinity.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 28 (1983), S. 335-342 
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The degradability of three high molecular weight polycaprolactones (M̄w = 35,000, 18,600, and 7,130) and one low molecular weight polycaprolactone diol(M̄w = 2060) by mixed and pure cultures of microorganisms was assayed. A yeast, Cryptococcus laurentii, a gram-negative rod, Acinetobacter calcoaceticus var. lwoffi, and a gram-positive coryneform rod were used in the pure culture assays. The analysis of degradation by gel permation chromatography (GPC) allowed for quantitation independent of the growth of the organisms or the addition of supplementary growth factors. GPC analysis showed that the degradation effected by pure cultures was often enhanced when alternate carbon sources were present. This was not the case for mixed cultures. Mixed cultures. Mixed cultures completely metabolized polymer breakdown products while in some cases pure cultures did not.
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Philadelphia : Wiley-Blackwell
    Journal of Cellular and Comparative Physiology 11 (1938), S. 433-454 
    ISSN: 0095-9898
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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