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  • 1
    Digitale Medien
    Digitale Medien
    Relationship between AgNORs and cell proliferation studied by flow and image cytometry
    Amsterdam : Elsevier
    Biology of the Cell 73 (1991), S. 45 
    Details
    ISSN: 0248-4900
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie
    Materialart: Digitale Medien
    URL: http://linkinghub.elsevier.com/retrieve/pii/0248-4900(91)90243-G
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  • 2
    Digitale Medien
    Digitale Medien
    Aspects of Anterior Pituitary Growth, with Special Reference to Corticotrophs (1999)
    Springer
    Pituitary 1 (1999), S. 257-268 
    Details
    ISSN: 1573-7403
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The spatial and proportional representation of the various cell groups in the anterior pituitary is fairly constant, although it may differ between sexes. Recognizable changes occur in a number of physiological and pathological situations. The relative roles of hormones and growth factors in these processes are not fully elucidated, nor are their kinetics. In this paper, published work on basal proliferation, growth factor expression and the growth of specific cell types are reviewed. In addition, we present new data to indicate that the maximum level of proliferation in the anterior pituitary of the male Sprague-Dawley occurs around 28 days. We have also demonstrated a circadian rhythm of mitosis in the adult male, with a peak around 1100h. Cell kinetic analysis suggests a duration for G2 of about 2 hours, and for S phase of 10 1/2 to 11 hours. Finally, we provide data which confirm that the expansion of the corticotroph population after bilateral adrenalectomy is partly the result of an early proliferative response in both corticotrophs and other pituitary cells. Our data also suggest that a further expansion takes place which may reflect differentiation of a population other than committed corticotrophs.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1009950308561
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  • 3
    Digitale Medien
    Digitale Medien
    Expression of argyrophilic nucleolar organizer regions (AgNORs) can be used to assess cellular proliferation as shown in rat thymic sections (1993)
    Springer
    Journal of molecular histology 25 (1993), S. 548-553 
    Details
    ISSN: 1573-6865
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Silver-stained nucleolar organizer regions (AgNORs) were studied in thymic sections from 4- and 30-day-old rats. By direct examination under the light microscope cells with a low or high content of AgNORs (type I and type II cells, respectively) were identified and their relative numbers calculated. The mean area of AgNORs per cell was calculated for each type of cell and age group. Additionally, the proportion of cells labelled with bromodeoxyuridine was calculated in sections from the same animals. Visual identification of type I and type II cells was confirmed by a significant (p 〈 0.01) difference in the mean AgNOR area in both types of cell. Both the proportion of cells with a high expression of AgNORs (type II cells) and that of bromodeoxyuridine-labelled cells were significantly greater (p 〈 0.01) in 4-day-old rats than in 30-day-old rats. A significant correlation was found between both variables (R2 = 0.45;p = 0.002), the relation being best between both variables and age (R2 = 0.91;p = 〈 0.001). These data offer support for an easy interpretation of the AgNOR reaction in which the proportion of cells with a high expression of AgNORs can be used as an index of proliferative activity in a tissue sample.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02388062
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  • 4
    Digitale Medien
    Digitale Medien
    Expression of argyrophilic nucleolar organizer regions (AgNORs) can be used to assess cellular proliferation as shown in rat thymic sections (1993)
    Springer
    Journal of molecular histology 25 (1993), S. 548-553 
    Details
    ISSN: 1573-6865
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Silver-stained nucleolar organizer regions (AgNORs) were studied in thymic sections from 4- and 30-day-old rats. By direct examination under the light microscope cells with a low or high content of AgNORs (type I and type II cells, respectively) were identified and their relative numbers calculated. The mean area of AgNORs per cell was calculated for each type of cell and age group. Additionally, the proportion of cells labelled with bromodeoxyuridine was calculated in sections from the same animals. Visual identification of type I and type II cells was confirmed by a significant (p 〈 0.01) difference in the mean AgNOR area in both types of cell. Both the proportion of cells with a high expression of AgNORs (type II cells) and that of bromodeoxyuridine-labelled cells were significantly greater (p 〈 0.01) in 4-day-old rats than in 30-day-old rats. A significant correlation was found between both variables (R2 = 0.45; p = 0.002), the relation being best between both variables and age (R2 = 0.91; p = 〈 0.001). These data offer support for an easy interpretation of the AgNOR reaction in which the proportion of cells with a high expression of AgNORs can be used as an index of proliferative activity in a tissue sample.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00173052
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  • 5
    Digitale Medien
    Digitale Medien
    Quantification of the cellular proliferation on freshly dispersed cells from rat anterior pituitaries afterin vivo andin vitro labelling with bromodeoxyuridine (1992)
    Springer
    Journal of molecular histology 24 (1992), S. 137-143 
    Details
    ISSN: 1573-6865
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The labelling index i.e., the proportion of cells in S phase of the cell cycle, has been calculated in cytospin preparations of rat anterior pituitary cells after labelling eitherin vivo orin vitro with the thymidine analogue bromodeoxyuridine (BrdU). The aims of this work were (1) to check whether enzymatic digestion interferes with the incorporation of BrdU into S phase cells and/or whether it has any deletereous effect on the immunohistochemical detection of cells that have already incorporated BrdU, and (2) to check the viability of simultaneous staining for BrdU and markers for the different types of pituitary cells in the cytospins. No statistical difference was found between the labelling index afterin vivo orin vitro labelling with BrdU. Identification of doubly-immunostained cells was straightforward and up to 40% of BrdU-labelled cells were immunopositive for pituitary hormones. It is suggested that cytospin preparations from biopsy samples may be used to study cellular proliferation without exposing the patient to the hazardous effects of BrdU infusion and without the interference of cell culture methods.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01047463
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  • 6
    Digitale Medien
    Digitale Medien
    Cellular proliferation in the anterior pituitary of the rat during the postnatal period (1990)
    Springer
    Cell & tissue research 261 (1990), S. 333-338 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Cell proliferation ; Anterior pituitary ; Postnatal development ; Immunohistochemistry ; Rat (Sprague-Dawley)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Cellular proliferation in the anterior pituitary of 2-, 8-, 15- and 30-day-old rats was examined by injection of bromodeoxyuridine 1 h before autopsy. Bromodeoxyuridine incorporated into DNA was detected immunohistochemically by use of a monoclonal antibody. The highest rate of cell proliferation was found in 2-day-old animals; it decreased thereafter during the postnatal period. Possible toxic effects of colchicine on cellular proliferation were examined. Colchicine treatment (10 mg/kg in 8- and 30-day-old animals) significantly decreased the number of bromodeoxyuridine-labelled cells/mm2 in 8-day-old rats. Some sections were doubly immunostained for bromodeoxyuridine and various pituitary hormones. The proportion of doubly-immunostained cells to all proliferating cells was generally low, ranging from 23% at 2 days to 32% at 30 days of age.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00318674
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