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  • 1
    Electronic Resource
    Electronic Resource
    The relationships between leptin and inflammatory cytokines in women with pre-eclampsia (2001)
    Oxford, UK : Blackwell Science Ltd
    BJOG 108 (2001), S. 0 
    Details
    ISSN: 1471-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Objective To compare maternal serum leptin concentration in women with pre-eclampsia and women with normal pregnancy, and to evaluate the relationships between leptin and several inflammatory cytokines.Design Prospective clinical study.Setting University Hospital of Puerto Real, Cadiz, Spain.Sample Twenty-seven women with pre-eclampsia and 25 normotensive pregnant women.Methods Maternal serum levels of TNF-α, TGF-β1, interleukin 6, and leptin were measured using a commercially available immunoassay.Main outcome measures Maternal serum levels of leptin and its relationship to levels of TNF-α, TGF-β1 and interleukin 6.Results Maternal serum leptin levels were higher in women with pre-eclampsia, but this difference was of borderline statistical significance (median 15.9ng/mL; interquartile range (5.4–31.9) vs 30.5ng/mL (13.9–64) (P=0.05). The concentration of maternal serum leptin was correlated with pre-gestational and gestational body mass index (r=0.45, P=0.03; r=0.44, P=0.04), TNF-α concentration (r=0.57, P=0.002) and interleukin-6 level (r=0.46, P=0.02) in women with normal pregnancy. In women with pre-eclampsia, only a significant correlation between maternal serum leptin level and TNF-α concentration (0.47, P=0.01) was found.Conclusions The level of maternal serum leptin is increased and correlates positively with the level of TNF-α in women with pre-eclampsia. In women with a normal pregnancy leptin levels not only correlate with TNF-α, but also with IL-6 and body mass index.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-0528.2001.00284.x
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  • 2
    Electronic Resource
    Electronic Resource
    Limited acclimation of photosynthesis to blue light in the seaweed Gracilaria tenuistipitata (2002)
    Oxford, UK : Munksgaard International Publishers
    Physiologia plantarum 114 (2002), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Changes in photosynthetic capacity of the seaweed Gracilaria tenuistipitata Zhang et Xia acclimated to monochromatic blue light were studied. For this purpose, affinity for external inorganic carbon, light use efficiency, carbonic anhydrase (CA; EC 4.2.1.1) activity and content of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco; EC 4.1.1.39) were determined in thalli acclimated to 45 µmol m−2 s−1 of blue light. Thalli cultured in white light of the same photon fluence rate were used as a control. Lower maximal photosynthetic rates (i.e. at light and carbon saturation) were obtained in the thalli cultured in blue light. Apparently, this lower photosynthetic capacity was not due to differences in affinity and/or capacity for use of external dissolved inorganic carbon (DIC) since (1) CA activity did not change significantly and (2) similar values of photosynthetic conductance for DIC at alkaline pH were obtained (0.95 × 10−6 m s−1). In addition, the pool size of Rubisco was not modified by the blue light treatment since there were no significant differences in Rubisco content between white (12.14% of soluble proteins) and blue light (12.13% of soluble proteins) treatments. In contrast, Fv/Fm was increased by 11% and photosynthetic efficiency for oxygen production was reduced by 50% in blue light. This absence of correlation between quantum yields for maximum stable charge separation of photosystem II and oxygen evolution suggests that blue light promote changes in rates of photosynthetic electron flow.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1399-3054.2002.1140319.x
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  • 3
    Electronic Resource
    Electronic Resource
    Affinity for inorganic carbon of Gracilaria tenuistipitata cultured at low and high irradiance (2000)
    Springer
    Planta 210 (2000), S. 758-764 
    Details
    ISSN: 1432-2048
    Keywords: Key words: Carbonic anhydrase –Gracilaria– Irradiance – pH – Photosynthesis – Ribulose-1,5-bisphosphate carboxylase/oxygenase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Regulation by irradiance level of the mechanism for dissolved inorganic carbon (DIC) acquisition was examined in the red macroalga Gracilaria tenuistipitata Zhang et Xia. For this purpose, affinity for external DIC, carbonic anhydrase (CA; EC 4.2.1.1) activity and content of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco; EC 4.1.1.39) were determined in thalli grown at 45 and 500 μmol photons m−2 s−1. Oxygen evolution rates declined by 50% when the medium pH was changed from 8.1 to 8.7, and the pH compensation point attained was ca. 9.2. These characteristics were unaffected by the light treatments. In contrast, photosynthetic conductance for DIC at pH 8.7 was doubled in thalli grown at high irradiance compared with those grown at low irradiance (to 0.74 × 10−6 from 0.33 × 10−6 m s−1). Photosynthetic rates at saturating DIC concentration were also higher by 60% in thalli grown at high irradiance. These differences could not be attributed to changes in the use of external DIC, since external CA activity did not vary. Although the irradiance level did not modify the pool size of Rubisco, Rubisco content expressed on a chlorophyll a basis was almost doubled at high irradiance. These results likely indicate that the internal transport of DIC towards the active-site of Rubisco, rather than the external use of DIC, is enhanced in the thalli grown at high irradiance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004250050677
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  • 4
    Electronic Resource
    Electronic Resource
    Light quality affects morphology and polysaccharide yield and composition of Gelidium sesquipedale (Rhodophyceae) (1998)
    Springer
    Journal of applied phycology 10 (1998), S. 323-332 
    Details
    ISSN: 1573-5176
    Keywords: Gelidium sesquipedale ; light quality ; branch ; agar ; starch
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Morphology and polysaccharide characterization of Gelidium sesquipedale (Clem.) Bornet et Thuret were studied in cultures grown under various light qualities. White light (WL), blue light (BL) and red light (RL) (all at photon fluence rate of 40 μmol m-2 s-1) were used for the study of morphological characteristics, and in addition yellow light (YL) for polysaccharide characterization. RL and BL induced a proliferating growth, which resulted in bushy plants under RL. Cortical cells of BL-grown plants were smaller and presented a higher density per unit area, whereas those of WL- and RL-grown alga were larger. Medullary cells followed the inverse pattern. Light quality also affected polysaccharide yield and composition, with the yield being higher under BL, RL or YL than WL. Most of the polysaccharide was extracted in distilled water at 100 °C, while a low amount was solubilized at 22 °C and 120 °C. Extracts from BL-grown alga presented the highest galactan content. The starch concentration was lower in extracts from RL-, BL- and YL-cultivated alga than in those from the initial plants. The degree of substitution with methoxyl groups and precursor was very low in all the agar fractions, but fractions extracted from BL- and WL-grown alga were more substituted by precursor. The highest sulfate content was reached under BL (about 9% w/w) and the highest 2-O-methyl-3,6-anhydro-L-galactose and 6-O-methyl-D-galactose content were found in extracts from alga grown under YL.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008042904972
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