ISSN:
0173-0835
Keywords:
Three dimensional imaging
;
Electrophoresis
;
DNA
;
Confocal detector
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
We have measured the three-dimensional distribution of DNA fragments within an electrophoretic band confined by a glass microchannel. The measurements were made using a confocal microscope and a photon-counting photo-multiplier detector. A DNA sequencing standard was loaded into glass micro-channel plates containing polyacrylamide gel. The measurements were made by scanning the plates in three dimensions using a mechanical stage under computer control, while electrophoresis was taking place. We found that the distribution of DNA was the same for all the bands measured in the sequencing ladder with an approximate Gaussian distribution along all three axes. These measurements are important to understand what physical forces shape electrophoretic bands confined by a channel and they also aid in the design of high throughput DNA sequencers.
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.11501601303
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