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  • 1
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Serine/threonine protein kinases (STPKs) represent a burgeoning concept in prokaryotic signaling and have been implicated in a range of control mechanisms. This paper describes the enzymatic and molecular characterization of PknH, a mycobacterial STPK. After cloning and expression as a Glutathione-S-transferase fusion protein in E. coli, PknH was found to phosphorylate itself and exogenous substrates like myelin basic protein and histone. The kinase activity of PknH was inhibited by the kinase inhibitors staurosporine and H-7. The results confirmed that PknH is a transmembrane protein and is restricted to members of the Mycobacterium tuberculosis complex. In addition, transcriptional analysis of pknH in M. tuberculosis under various stress conditions revealed that exposure to low pH and heat shock decreased the level of pknH transcription significantly. This is the first report describing differential expression of a mycobacterial kinase in response to stress conditions which can indicate its ability to regulate cellular events promoting bacterial adaptation to environmental change.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    International archives of occupational and environmental health 63 (1991), S. 21-26 
    ISSN: 1432-1246
    Keywords: Agate industry ; Respiratory morbidity ; Pneumoconiosis ; Silica dust
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A random sample of 342 workers engaged in chipping and grinding of agate stones were surveyed in a cross-sectional study to assess the prevalence of respiratory morbidity in the agate industry. The findings were compared with those obtained in controls. The study showed a significantly higher prevalence of lung diseases among agate workers than among controls (63.4% vs 35.5%, P 〈 0.001). The respiratory morbidity in agate workers and controls was maximal in the group aged 31+ years (83.3% and 42.2%, respectively). The prevalence of pneumoconiosis in agate workers (18.4%) was highly significant as compared with controls, in whom not a single case was found (P 〈 0.001). However, there was no significant difference between the prevalence of pneumoconiosis in men and women of the exposed group (17.9% vs 19.6%). Among the cases of pulmonary diseases in agate workers, pneumoconiosis formed the largest group (18.4%), whereas among controls it was tuberculosis (12.1%). The prevalence of pulmonary tuberculosis was very high in both agate workers and controls (15.5% and 12.1%, respectively), probably because of poor socio-economic and unhygienic living conditions. The prevalence of chronic bronchitis was found to be higher among the control population as compared with the exposed group (6.7% vs 2.6%). It therefore appeared that agate dust had no role in precipitating chronic bronchitis. However, bronchial asthma appeared to have been aggravated due to agate dust, as the risk among agate workers was 7-fold that found among the controls. The prevalence of pneumoconiosis showed a dose-response relationship in both male and female agate workers. Pulmonary tuberculosis and bronchial asthma did not show any significant trend in relation to the duration of exposure. Other lung diseases were also independent of exposure. As far as occupations are concerned, grinders were found to suffer more from pneumoconiosis (21.9%) than did chippers (7.3%, P 〈 0.05); this was because the respirable dust concentration at the workplace of grinders was 6-fold that at the workplace of chippers. Acute bronchitis was also more prevalent in grinders (P 〈 0.05). However, tuberculosis and asthma did not vary significantly between the two occupational groups.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 332 (1988), S. 526-527 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Three types of radicals have been detected by ESR spectroscopy in a range of flint samples1"4. One type, which may be termed intrinsic, is based on the silica itself, and is formed by ionizing radiation. These radicals are essentially electron-capture and electron-loss centres, which in principle ...
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 328 (1987), S. 833-834 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Radicals in solids can be generated by grinding, generally at low temperatures. These radicals are detected by ESR spectros-copy, but the mechanism of their formation is not clear. No examples in which trapped radicals have been formed by the related process of cutting have been reported. Here we ...
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Astrophysics and space science 218 (1994), S. 249-265 
    ISSN: 1572-946X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract This article describes in detail a burst from PSR 0950+08 on July 29, 1992. This event was observed by two radio telescopes (separated by ~ 200 km) operating at 103 MHz. There exists a very convincing indirect evidence that at the same time the pulsar also emitted large X-ray flux. The X-ray flux during the event compares with that during a solar X-ray flare. During the event the Sun was extraordinarily quiet as the solar X-ray flux ≤ 3 · 10−7 W/m2 only was observed. The cause for the burst is quite unknown and may be complex. However, a possibility of accretion of a comet-like object by pulsar may provide reasonable explanation of the observations. These results open some interesting questions about the pulsar physics.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Water, air & soil pollution 32 (1987), S. 379-387 
    ISSN: 1573-2932
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The protective role of 18 amino acids on the acute toxicity of Cu in a fresh water cladoceran, Daphnia magna is reported. One hundred percent mortality in 48 hr of exposure occured at 0.56 mg L−1 of Cu or higher. A dose of each tested amino acid (10 mg L−1 was found to significantly neutralize the toxic effect of Cu as evidenced by a decrease in mortality and a significant increase in the median survival time (LT50) value). The 48 hr LC50 value was 0.093 mg L−1 for Cu alone, while the LC50s with Cu plus amino acids ranged from 0.438 to 2.516 mg L−1 of Cu, suggesting a 4.7 to 27 fold decrease in acute toxicity of Cu. A significant difference between LC50 of Cu alone and Cu with amino acids was observed. The role of amino acids for protection against heavy metal pollution stress in aquatic animals is discussed.
    Type of Medium: Electronic Resource
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