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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of the American Chemical Society 89 (1967), S. 3643-3645 
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of the American Chemical Society 89 (1967), S. 5976-5977 
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-1432
    Keywords: 5S rRNA ; Phylogenetics ; Nonuniform rates ; Computer simulation ; Precambrian evolution ; Molecular evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effects of temporal (among different branches of a phylogeny) and spatial (among different nucleotide sites within a gene) nonuniformities of nucleotide substitution rates on the construction of phylogenetic trees from nucleotide sequences are addressed. Spatial nonuniformity may be estimated by using Shannon's (1948) entropy formula to measure the Relative Nucleotide Variability (RNV) at each nucleotide site in an aligned set of sequences; this is demonstrated by a comparative analysis of 5S rRNAs. New methods of constructing phylogenetic trees are proposed that augment the Unweighted Pair-Group Using Arithmetic Averages (UPGMA) algorithm by estimating and compensating for both spatial and temporal nonuniformity in substitution rates. These methods are evaluated by computer simulations of 5S rRNA evolution that include both kinds of nonuniformities. It was found that the proposed Reference Ratio Method improved both the ability to reconstruct the correct topology of a tree and also the estimation of branch lengths as compared to UPGMA. A previous method (Farris et al. 1970; Klotz et al. 1979; Li 1981) was found to be less successful in reconstructing topologies when there is high probability of multiple mutations at some sites. Phylogenetic analyses of 5S rRNA sequences support the endosymbiotic origins of both chloroplasts and mitochondria, even though the latter exhibit an accelerated rate of nucleotide substitution. Phylogenetic trees also reveal an adaptive radiation within the eubacteria and another within the eukaryotes for the origins of most major phyla within each group during the Precambrian era.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 137 (1984), S. 200-204 
    ISSN: 1432-072X
    Keywords: Rhodomicrobium vannielii ; Photosynthetic bacteria ; Pentacyclic triterpenoids ; Hopanes ; Fernenes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Triterpenoids have been isolated from the lipids of the anaerobic, photosynthetic bacterium, Rhodomicrobium vannielii. These compounds constitute various hopanoids, including hydrocarbons, 22-, 29-and 3β-hydroxy, 3-keto-, C-3 and C-21 methyl-triterpenoids, as well as fernenes.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-072X
    Keywords: Chlorobium limicola ; Aryl carotenoids ; Chlorobactene biosynthesis ; Nicotine ; Diphenylamine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The biosynthesis of the aryl carotenoid, chlorobactene, was examined in the green sulfur bacterium, Chlorobium limicola f. thiosulfatophilum. Nicotine, which was used to inhibit carotenoid cyclization, caused the accumulation of the acyclic carotenoid, lycopene. Cells reincubated in fresh medium, after removal of nicotine, synthesized chlorobactene more readily from newly synthesized lycopene rather than from the pool of lycopene accumulated during nicotine inhibition. When the cells were reincubated in the presence of diphenylamine, which inhibited de novo carotenogenesis, a portion of the lycopene which had accumulated during nicotine inhibition was converted into chlorobactene. There was no evidence that neurosporene, rather than lycopene, was the precyclization intermediate. The involvement of γ-carotene as the cyclic precursor of chlorobactene also was shown. The pathway for chlorobactene biosynthesis is discussed in terms of a possible arrangement of the enzymes involved in carotenoid biosynthesis.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The pigments of the endosymbiotic algae in Cyanophora paradoxa (colorless cryptomonad) and Glaucocystis nostochinearum (colorless Chlorophyceae) and two Rhodophyceae, Porphyridium aerugineum and Asterocytis ramosa have been examined. Both endosymbionts contain chlorophyll a, β-carotene, zeaxanthin, C-phycocyanin and small amounts of allophycocyanin. Porphyridium has been shown to contain chlorophyll a, β-carotene and zeaxanthin, as does Asterocytis ramosa. The biliproteins of Porphyridium have not been examined, but evidence is presented to suggest that Asterocytis ramosa may contain R-phycocyanin and possibly C-phycocyanin. The taxonomic implications of these results have been discussed, especially with regard to cyanome symbionts and their evolution from and classification with the Cyanophyceae.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1573-5176
    Keywords: Tetraselmis ; small subunit ribosomal RNA ; green algae ; feedstock ; mariculture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This reports the first sequence for a small subunit (185) ribosomal RNA gene (SSU rDNA) fromTetraselmis convolutae.Tetraselmis (=Platymonas) species are important feed stocks for many organisms important in mariculture. This sequence is an essential molecular marker to distinguish the DNAs ofTetraselmis from the DNAs of other organisms in the culture. In addition thisTetraselmis SSU rDNA sequence provides an important entry for the gradually expanding database of symbiotic microalgae.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Photosynthesis research 22 (1989), S. 295-301 
    ISSN: 1573-5079
    Keywords: Photosystem II ; D1/D2/cytochrome b-599 ; thermoluminescence ; charge recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Formation of thermoluminescence signals is characteristics of energy- and charge storage in Photosystem II. In isolated D1/D2/cytochrome b-559 Photosystem II reaction centre preparation four thermoluminescence components were found. These appear at -180 (Z band), between -80 and -50 (Zv band), at -30 and at +35°C. The Z band arises from pigment molecules but not correlated with photosynthetic activity. The Zv and -30°C bands arise from the recombination of charge pairs stabilized in the Photosystem II reaction centre complex. The +35°C band probably corresponds to the artefact glow peak resulting from a pigment-protein-detergent interaction in subchloroplast preparations (Rózsa Zs, Droppa M and Horváth G (1989) Biochim Biophys Acta 973, 350–353).
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1573-5117
    Keywords: photobioreactor ; dynamic irradiance ; Lagrangian float ; microalgae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An inexpensive and simple, analytical microalgal photobioreactor with a highly controllable, dynamic, spectrally attenuated light source is described. Spectral attenuation is achieved through the introduction of a variable thickness of CuSO4 solution between the photobioreactor and a light source. The level of liquid is precisely determined via a computer-controlled peristaltic pump, which can be programmed to pump at a variety of rates. The resultant light fields consist of a wide range of irradiance intensities with concomitant spectral narrowing, which closely mimics modeled clear water attenuation patterns. Irradiance dynamics associated with virtually any mixing regime can be achieved. The culturing apparatus of the analytical photobioreactor is based on traditional flat-plate, photobioreactor design, but with several modifications: (1) The light path has been reduced to 1 cm to assure a uniform light field is experienced by all phytoplankton at relatively low cell densities; (2) carbon dioxide concentrations are kept constant through a negative feedback mechanism that pulses CO2 into a constant air stream when culture media pH rises above a set point; (3) temperature is controlled in a similar manner, through the addition of cooling water to a water jacket in response to an increase in culture media temperature. This design is intended for use in photophysiological and bio- physical studies of microalgae under highly controlled culture conditions. It should prove easily adaptable to any number of more complex configurations.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 0075-4617
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Bonding between Chromophore and Protein in Biliproteins, I. - Degradation Experiments and Spectral Investigations on BiliproteinsDegradation experiments with nine different biliproteins show that ring A of the chromophore and one of the middle rings (in phycoerythrin identified as ring C) are always covalently linked with the apoprotein. According to spectral investigations, the chromophore of phycoerythrin contains six, and that of phycocyanin nine conjugated double bonds. One additional double bond is formed during splitting of the chromophore with boiling methanol. Partial structure 11 containing the linkage to the apoprotein is proposed for the phycoerythrin chromophore on the basis of kinetics of isomerization to a urobilin.
    Notes: Abbauversuche an neun verschiedenen Biliproteiden zeigen, daß immer jeweils Ring A des Chromophors und einer der mittleren Ringe (bei Phycoerythrinen als Ring C identifiziert) mit dem Protein kovalent verknüpft sind. Nach Spektraluntersuchungen besitzt der Chromophor des Phycoerythrins sechs, der des Phycocyanins neun konjugierte Doppelbindungen. Bei der Abspaltung der Chromophore mit siedendem Methanol kommt jeweils eine Doppelbindung hinzu. Für den Phycoerythrin-Chromophor wird aufgrund der Kinetik der Isomerisierung in ein Urobilin die Teilstruktur 11 vorgeschlagen, die die Bindungsstelle zum Protein berücksichtigt.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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