ISSN:
1365-2222
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Medizin
Notizen:
Background The underlying mechanisms of elevated IgE level in atopic patients are still obscure, however, extensive efforts have been tried to identify an immunological parameter as a predictor of atopy.Objective This study compared the difference in cytokine production by cord blood mononuclear cells between new borns with high-risk of allergy (family allergy score, FAS ± 3) and those with low-risk (FAS = 0).Methods Following stimulation with PHA(100 μg/mL) and PMA (1 ng/mL), the cytokines produced by cord blood CD4+ T cells in the presence of monocytes were measured by ELISA kits and the mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) technique.Results Our results showed: CD4+ T cells in the presence of monocytes and isolated monocytes from the high-risk group produced a much greater amount of IL-6, either spontaneously or after stimulation, than did those of the low-risk group; CD4+ T cells of low-risk group produced a significantly greater amount of interferon gamma (IFNγ) than did those from the high-risk group; IL-4 cannot be detected by ELISA kit, and only a trace amount of IL-4 mRNA was detected by RT-PCR technique; cord blood basophils stimulated with PHA and PMA could produce a significant amount of IL-4; there was an inverse correlation between the production of IFNγ and cord blood IgE level (high-risk group, r - 0.647, n = 17) and the number of natural killer (NK) cells (CD3− CD16+ CD56+) was signiticantly lower in high-risk group than for low-risk group.Conclusion Our data suggested increased production of IL-6 and decreased production of IFNγ of cord blood mononuclear cells appear to be the hallmark of newborns from the high-risk population.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1111/j.1365-2222.1996.tb00555.x
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