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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    BBA - Enzymology 132 (1967), S. 361-369 
    ISSN: 0005-2744
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 150 (2004), S. 0 
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 42 (1986), S. 3-8 
    ISSN: 1570-7458
    Keywords: Bombyx mori ; silkworm ; Spirulina platensis ; spiral blue-green algae ; red fluorescent protein ; nuclear polyhedrosis virus ; phospholipase C ; gut juice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Les protéines de l'intestin moyen de Bombyx mori, nourri avec un aliment artificiel contenant de la poudre séchée de l'algue bleue spiralée, Spirulina pratensis, avaient une très faible fluorescence. Cesi indique que la chlorophylle a de cette algue n'est pas utilisable pour la synthèse de la protéine fluorescente rouge (RFP), considérée comme une protéine antivirale efficace de l'intestin moyen. Après séparation par électrophorèse sur gel de polyacrylamine DISC plusieurs bandes de la protéine de l'intestin moyen de B. mori élevé sur mûrier émettaient une fluorescence rouge en lumière U.V. On peut conclure que RFP est un complexe plutôt qu'une protéine particulière de l'intestin moyen. Les analyses enzymatiques ont montré que la phospholipase C et non les protéases ont une activité antivirale dans la RFP purifiée provenant de vers à soie nourris à partir de mûrier ou sur régime artificiel.
    Notes: Abstract Midgut proteins from the silkworms, Bombyx mori, fed on an artificial diet containing dried powder of the spiral blue-green alga, Spirulina platensis, had a very low fluorescence intensity. This indicated that chlorophyll in this alga is not utilizable for synthesizing the red fluorescent protein (RFP) which has been considered as an effective antiviral protein in silkworm midgut. Many electrophoretic bands of the midgut proteins from the mulberry-fed silkworms emitted red fluorescence under UV light. It is thus concluded that the RFP as a whole is more than one midgut protein. Enzymatic analyses showed that silkworms fed on mulberry leaves and artificial diets had similar protease activities, but they had different phospholipase C activities. Therefore, it is suggested that the antiviral activity of purified RFP in the silkworm gut juice is attributable to phospholipase C.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1741-2765
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Two correlative methods are developed for the computer-aided photoelastic analysis of orthogonal 3D textile composites comprising 6 mm×6 mm highly heterogeneous unit cells in-plane. The first method, presented in Part 1, is used for the whole-field determination of isochromatics and isoclinics; the second method, presented in Part 2, is applied for subsequent photoelastic stress analysis. Part 1 delineates precisely how one circular polariscope and two plane polariscope photoelastic images are implemented to extract the isochromatic parameterR and isoclinic parameter α using the digital image processing technique. The photoelastic experiments were performed with orthogonal 3D textile composite as well as aluminum plates containing a hole under the uniaxial tension loading. The obtained values ofR and α, for the case of the aluminum plate, are smoothly distributed over the field and show consistent agreement with those computed from finite element analysis (FEM). In the case of the composite plate, the global distribution ofR and α also agree well with FEM results employing the homogenized composite properties. However, bothR and α exhibit local variations according to the 3D fabric structure, and the varying amplitude of α is relatively larger than that ofR.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1741-2765
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract An approach combining least squares methods and finite element methods (FEM) is presented for subsequent photoelastic stress analysis of orthogonal 3D textile composites withR and α obtained in Part 1. Through this approach, these photoelastic stresses are obtained over a region of interest as if the composites were homogeneous materials. The least squares method is used for requiring the solution strain fields to best correlate with the distribution of the two photoelastic strain data of ɛ x − ɛ y and γ xy calculated directly from the measuredR and α. The FEM uses the homogenized composite properties to construct the nodal force equilibrium equations as constraints in the least squares formulation. As a result of combining this least squares method and FEM with lagrange multipliers, a linear system of equations is formulated with the unknown nodal displacements. Once these nodal displacements are solved, the strains and stresses can be calculated through FEM formulations. This approach is tested with the two experimental results completed in Part 1 for the aluminum and composite plates. The stresses obtained for the aluminum plate show close agreement with those obtained with the plain FEM computation. In the case of the orthogonal 3D composite plate, the local variations as observed inR and α are already necessarily eliminated from these solved photoelastic stresses. Furthermore, these stresses also match well with those computed with the plain FEM from the homogenized composite properties.
    Type of Medium: Electronic Resource
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