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  • 1
    Electronic Resource
    Electronic Resource
    Membrane Currents Induced in Xenopus Oocytes by the C-Terminal Fragment of the β-Amyloid Precursor Protein (1996)
    Oxford, UK : Blackwell Science Ltd
    Journal of neurochemistry 66 (1996), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: There is mounting evidence that at least some of the neurotoxicity associated with Alzheimer's disease (AD) is due to proteolytic fragments of the β-amyloid precursor protein (βAPP). Most research has focused on the amyloid β protein (Aβ), which has been shown to possess ion channel activity. However, the possible role of other cleaved products of the βAPP is less clear. We have investigated the ability of various products of βAPP to induce membrane ion currents by applying them to Xenopus oocytes, a model system used extensively for investigating electrophysiological aspects of cellular, including neuronal, signalling. We focussed on the 105-amino-acid C-terminal fragment (CT105) (containing the full sequence Aβ), which has previously been found to be toxic to cells, although little is known about its mode of action. We have found that CT105 is exceedingly potent, with a threshold concentration of 100–200 nM, in inducing nonselective ion currents when applied from either outside or inside the oocyte and is more effective than either βAPP or the Aβ fragments, β25–35 or β1–40. The ion channel activity of CT105 was concentration dependent and blocked by a monoclonal antibody to Aβ. These results suggest the possible involvement of CT105 in inducing the neural toxicity characteristic of AD.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1471-4159.1996.66052034.x
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  • 2
    Electronic Resource
    Electronic Resource
    Megakaryothrombopoiesis During Ex Vivo Expansion of Human Cord Blood CD34+ Cells Using Thrombopoietin (2002)
    Oxford, UK : Blackwell Science Ltd
    Scandinavian journal of immunology 55 (2002), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Thrombopoietin (TPO) is one of the most promising stimulants for ex vivo expansion of haematopoietic stem cells. Previously, we have found that TPO induces a characteristic pattern of apoptosis during ex vivo expansion of human cord blood (CB) CD34+ cells and that the TPO-induced apoptotic cells belong to megakaryocyte (MK) lineage. In this study, we have examined the maturation of MK and platelet production in association with the TPO-induced apoptosis. CD34+ cells, purified from human CB, were expanded in serum-free conditions stimulated with TPO. Apoptosis was confirmed by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end labelling (TUNEL) assay and electron microscopy (EM). Simultaneous measurement of DNA content and immunophenotyping revealed that the cells with higher DNA content (〉8 N) constituted less than 5% of the CD41+ fractions until day 14, implying premature apoptosis of MKs before full polyploidization. Nevertheless, EM observation showed not only platelet territories but also newly produced platelets in which granules and microfilaments could be identified. Furthermore, flow cytometry demonstrated that the platelet fraction expressed P-selectin and an activation motif on GPIIb/IIIa recognized by monoclonal antibody PAC-1 upon stimulation with adenosine diphosphate (ADP). In addition, periodic acid-Schiff (PAS)-positive materials and nonspecific esterase activities could be demonstrated. Therefore, it is suggested that platelet production and the accompanying processes, rather than apoptosis only, be hastened during the ex vivo expansion of CB CD34+ cells when using TPO.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3083.2002.01031.x
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  • 3
    Electronic Resource
    Electronic Resource
    Hexamethylenetetramine–hydroquinone (1:1): correction of printer's error (1978)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 34 (1978), S. 700-700 
    Details
    ISSN: 1600-5740
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S056774087800388X
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Hexamethylenetetramine–hydroquinone (1:1) (1977)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 33 (1977), S. 2980-2982 
    Details
    ISSN: 1600-5740
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S056774087701005X
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  • 5
    Electronic Resource
    Electronic Resource
    Use of reciprocal modal vectors for nonlinearity detection (2000)
    Springer
    Archive of applied mechanics 70 (2000), S. 453-462 
    Details
    ISSN: 1432-0681
    Keywords: Key words Experiment ; modal analysis ; frequency response ; harmonic balance ; friction damping ; cubic stiffness
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Summary  This paper seeks to exploit the reciprocal modal vector orthogonality between experimentally-derived mode shapes and the corresponding measured frequency response functions in order to derive a criterion for the detection of structural non-linearities. Being based on the use of measured data and experimentally-derived quantities only, the method is directly applicable to practical engineering cases for which there are usually no spatial descriptions. A brief outline of the reciprocal modal vector theory is given first, followed by a short description of a frequency-domain nonlinear response simulation technique using a harmonic balance approach. A detailed study of a 4-DOF system with cubic stiffness and friction damping nonlinearities is presented next. It is shown that the proposed nonlinearity detection criterion is relatively insensitive to measurement noise. It is concluded that the method is effective for detecting nonlinear behaviour in a consistent and quantitative fashion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004199900078
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    Paper (German National Licenses)
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