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  • 1
    Electronic Resource
    Electronic Resource
    Microvascular changes in liver after ischemia-reperfusion injury (1994)
    Springer
    Digestive diseases and sciences 39 (1994), S. 1683-1690 
    Details
    ISSN: 1573-2568
    Keywords: liver ; microvasculature ; ischemia ; reperfusion ; misoprostol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Morphological changes in the hepatic microvasculature were studied in experimentally induced ischemia-reperfusion injury in the rat using a vascular casting technique. Partial hepatic ischemia was induced for 90 min followed by 24 hr of reperfusion. Microvascular casting was performed after 24 hr reperfusion by either intraarterial or intravenous infusion of acrylic resin (Mercox). After corrosion of the tissue, the cast was examined by scanning electron microscopy. Casts of normal livers showed good patency with no evidence of unfilled areas. The mean diameter of sinusoids was 14±3 µm with those in zone 1 slightly smaller than those in zone 3. Liver casts from rats subjected to ischemia and reperfusion resulted in gross disruption of normal architecture. The common characteristics seen in both prograde and retrograde casts were clusters of closed sinusoids around zones 2 and 3 of the liver acini, which resulted in cavities of various sizes. Varicosities were observed in some areas. The mean diameter of sinusoids in areas of patent microvascular structure (10±2 µm) was significantly smaller compared to those in normal livers (P〈0.001). Misoprostol given at 1 min before reperfusion markedly reduced the microvascular injury. The hepatic microvasculature was generally intact with mild focal unfilled areas. The majority of the sinusoids were of normal size and no clusters of blind ending sinusoids were detected. The present study shows that hepatic ischemia-reperfusion results in extensive microvascular injury in the liver. The protective effects of misoprostol against this injury may occur at the vascular level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02087776
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Misoprostol hepatoprotection against ischemia-reperfusion-induced liver injury in the rat (1992)
    Springer
    Digestive diseases and sciences 37 (1992), S. 1275-1281 
    Details
    ISSN: 1573-2568
    Keywords: misoprostol ; ischemia ; reperfusion ; hepatoprotection ; reactive oxygen metabolites
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The hepatoprotective effects of misoprostol, a PGE1 analog, against ischemiareperfusion liver injury were studied using a rat partial liver ischemia model. Serum ornithine carbamoyltransferase (OCT) and alanine aminotransferase (ALT) levels were determined as biochemical indices of injury. Hepatic cell necrosis was assessed histologically using tetranitroblue tetrazolium (TNBT) and hematoxylin and eosin (H&E) staining. With placebo treatment, 90 min of partial hepatic ischemia followed by 24 hr of reperfusion resulted in increased levels of serum OCT (760±521IU/liter) and ALT (4327 ±1982 IU/liter), while extensive hepatic necrosis was evident by TNBT and H&E staining. Treatment with two doses of 25 μg misoprostol/kg body weight at 1 min before ischemia and 1 min before reperfusion significantly reduced the serum levels of OCT and ALT (207±189 IU/liter, P〈0.01 and 2075±1217 IU/liter, P〈0.01, respectively) and hepatic necrosis. When a single dose of misoprostol was administered 1 min before reperfusion, similar protective effects were observed. However, when the treatment of misoprostol was delayed to 1 min after reperfusion, significantly less hepatoprotection was seen. Misoprostol exerted no hepatoprotection at all when it was administered at 5 min or later after reperfusion. These results demonstrate that misoprostol partially protects the liver against ischemia-reperfusion injury in the rat. The observation that the protective effect of misoprostol occurs only within the first minute of reperfusion suggests that its mechanism of action involves an early event in reperfusion injury, such as modifying the effects of reactive oxygen metabolites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01296572
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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