ISSN:
1365-2036
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
RT–PCR amplification of tumour-specific mRNA has been used for the detection of cancer cells in peripheral blood.〈section xml:id="abs1-2"〉〈title type="main"〉Aim:To evaluate the characteristics of the tumour specific mRNA species in peripheral blood of stomach cancer patients.〈section xml:id="abs1-3"〉〈title type="main"〉Methods:We analysed CEA, GalNAc-T, MUC-1, c-MET and hTERT mRNA expression in the stomach cancer cell lines and tissues, lymph nodes and peripheral blood of stomach cancer patients using RT–PCR.〈section xml:id="abs1-4"〉〈title type="main"〉Results:In RT–PCR analysis of the peripheral blood, 4%, 8%, 21%, 46%, and 100% of stomach cancer patients were positive for CEA, GalNAc-T, c-MET, hTERT and MUC-1 mRNA, respectively, but MUC-1 mRNA was also positive in all normal blood samples. The detection of hTERT mRNA was correlated with poor differentiation (P=0.01) and lymph node metastasis (P=0.009). The presence of c-MET mRNA was correlated with T stage (P=0.025), lymph node metastasis (P=0.036), distant metastasis (P=0.031), and stage of the stomach cancer (P=0.023).〈section xml:id="abs1-5"〉〈title type="main"〉Conclusions:Our study suggest that hTERT mRNA in peripheral blood can be a molecular marker for gastric cancer. We also showed that each molecular marker can be correlated with the clinicopathological features of the patients.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1046/j.1365-2036.16.s2.33.x
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