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  • 1
    Electronic Resource
    Electronic Resource
    Immunological evidence for the identity between the hsp27 estrogen-regulated heat shock protein and the p29 estrogen receptor-associated protein in breast and endometrial cancer (1991)
    Springer
    Breast cancer research and treatment 20 (1991), S. 33-42 
    Details
    ISSN: 1573-7217
    Keywords: breast cancer ; endometrial cancer ; estrogen receptor ; heat shock protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Previous studies have shown several similarities between an estrogen-regulated heat shock protein of 24,000–28,000 daltons (hsp27), and a cytoplasmic estrogen receptor-associated protein of 27,000–29,000 daltons (p29). These proteins have been studied by monoclonal antibodies generated in different laboratories. In the present report we have performed immunocytochemical and immunoblot studies to explore if the monoclonal antibodies against hsp27 (C11) and against p29 (ER-D5) may be identifying the same protein. Breast and endometrial carcinomas and normal endometrial samples were examined by immunocytochemistry (in mirror sections and by double-immunostaining). Identical hsp27 and p29 immunostaining intensity, distribution, and percentage of stained cells was demonstrated by immunocytochemistry. The antigens examined by the two antibodies appeared in the same cells. Cytosols from tumors analyzed by the Western blot technique revealed that the C11 and the ER-D5 antibodies recognized bands with identical electrophoretic mobility. Immunoprecipitation studies with one antibody, C11, followed by Western blot showed that the precipitate was reactive with both antibodies. Identical C11 and ER-D5 reacting spots were observed after blotting proteins separated by high resolution two-dimensional gel electrophoresis. In addition, p29 protein was induced by heat shock in the estrogen receptor negative MDA-MB-231 human breast tumor cell line. These results strongly suggest that the two proteins under investigation are identical.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01833355
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Effects of pimozide on the ultrastructure of the pars distalis in the rat (1979)
    Springer
    Cell & tissue research 199 (1979), S. 483-492 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary gland ; Rat ; Luteotroph cells ; Pimozide ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of pimozide, a dopamine receptor-blocking agent, were studied in the pars distalis of the rat. The animals received 100μg/100 g pimozide daily for 5, 10, 15, and 20 days. Pimozide induces striking ultrastructural changes after 5 days of treatment. The number of luteotroph (LTH) cells is significantly increased; they display characteristics of stimulation. The extrusion of granules into the intercellular space via exocytosis is frequently observed. The intercellular spaces are highly dilated, forming a lacunar system filled with an amorphous material, erythrocytes and involuted LTH cells. Transitional stages in the process of involution are observed in LTH cells. Luteotroph cells also form a syncytium. Twenty days after treatment the abovedescribed changes decrease in magnitude. The present findings suggest that pimozide stimulates the mechanism of synthesis and release in the luteotroph cells, an effect that is less evident with longer treatment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236084
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of pimozide on the ultrastructure of the pars intermedia in the rat (1981)
    Springer
    Cell & tissue research 217 (1981), S. 397-403 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary gland ; Rat ; MSH-cells ; Pimozide ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of pimozide, a dopamine receptor-blocking agent, were studied in the pars intermedia of the rat. The animals received 100 μg/100 g pimozide daily for 2, 5, 10, 15, and 20 days. Pimozide induces ultrastructural changes after 5 days of treatment. About 50% of the MSH-cells display characteristics of stimulation. Their cytoplasm is partially or totally depleted of secretory granules. The rough endoplasmic reticulum displays a network of interconnecting cisternae and ribbon-like structures. The well-developed Golgi complexes exhibit numerous dilatations of their cisternae, which contain electron-dense material. The nerve endings are not altered. Twenty days after treatment, the above-described changes have not decreased in magnitude. The present findings suggest that pimozide stimulates the mechanism of synthesis and release in some MSH-cells, most probably the elements underlying an inhibitory dopaminergic control.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233589
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    Paper (German National Licenses)
    Fulltext
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