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  • 1
    Electronic Resource
    Electronic Resource
    Methane oxidation potential and preliminary analysis of methanotrophs in blanket bog peat using molecular ecology techniques (1996)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 21 (1996), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract: The potential for methane oxidation was measured, and methanotroph gene sequences studied, in a peat core from the Moorhouse Nature Reserve, UK. Methane oxidation potential was observed in all depths of the peat core (down to 30 cm), and was inhibited by addition of acetylene, indicating the involvement of methane-oxidising bacteria. A peak of activity was shown in the 10–12 cm horizon, below which activity decreased with depth. Above this horizon, methane oxidation was relatively high and showed little change with depth. 16S rDNA libraries from several sections of the peat core were screened with methanotroph 16S rDNA probes designed to detect the genera Methylomonas, Methylococcus, Methylobacter and Methylosinus. Two clones, MHP14 and MHP17, hybridised strongly with the Methylosinus probe and upon complete sequencing and phylogenetic analysis were shown to group closely to the Methylosinus/Methylocystis genera of methanotrophs. However, the clones do form a distinct branch of their own, supported by BOOTSTRAP values, and may represent a novel group of acidophilic methanotrophs which have yet to be cultured.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.1996.tb00347.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    nifH genes in the obligate methane oxidizing bacteria (1988)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 49 (1988), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The hifH gene from Klebsiella pneumoniae, which codes for the Fe protein component of nitrogenase, was used as a probe to detect nifH homologues in total cellular DNA from 13 obligate methane oxidizing bacteria. All but one of those strains that had previously been shown capable of fixing dinitrogen contained sequences homologous to nifH. DNAs from three of the six non-diazotrophic strains were also found to possess such homology.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1988.tb02681.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Bacterial oxidation of propane (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 122 (1994), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The bacterial metabolism of propane and the pathway(s) involved are poorly understood, as the relative importance of terminal versus subterminal oxidation of propane, via propan-1-ol and propan-2-ol, respectively, is still unclear. In the case of bacteria, the ability to oxidize propane appears to be confined mainly to the Gram-positive Corynebacterium-Nocardia-Mycobaterium-Rhodococcus complex. Studies on propane oxidation have been hampered by a lack of firm enzymological data; for example, to date there are no reports of a purified propane oxygenase system. However, oxygenase activity has been confirmed by the production of propan-1-ol and/or propan-2-ol, and more recentlly by the co-oxidation of propene to 1,2-epoxypropane in cell extracts of propane-grown cells. Here, we review the use of genetic, biochemical and immunological techniques to assess the role(s) of terminal and subterminal oxidation in the metabolism of propane by Rhodococcus rhodochrous PNKb1 and present a general overview of the topic.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1994.tb07134.x
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Cloning of nitrogenase structural genes from the obligate methanotroph Methylococcus capsulatus (Bath) (1991)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 78 (1991), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Southern hybridization techniques were used to examine the DNA homologies between the three nitrogenase structural genes nifH, nifD and nifK of Klebsiella pneumoniae and DNA from the obligate methane oxidizing bacterium Methylococcus capsulatus (Bath). The high degree of homology between methanotroph DNA sequences and the KlebsiellanifH and nifD genes was used to isolate and clone the corresponding MethylococcusnifH and nifD genes. Subsequent restriction analysis revealed that all three nif structural genes were contiguous in the Methylococcu genome in the order nifH, nifD and nifK, as found for the majority of other diazotrophs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1991.tb04429.x
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    Paper (German National Licenses)
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