ISSN:
0006-3525
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The conformational properties of an analog of atrial naturiuretic factor, [Pro-10] ANF(7-23), were examined in H2O, H2O/DMSO-d6 (2/1), and DMSO-d6 using two-dimensional nmr techniques. The sequence differs from the native peptide by the absence of the exocyclic N- and C-terminal residues, and the substitution of a proline for a glycine at position 10 - a modification expected to reduce the conformational flexibility of this analog. The backbone proton nmr resonances were assigned from two-dimensional correlated spectroscopy (2D-COSY), relayed COSY, and 2D nuclear Overhàuser enhancement (NOE) experiments, and the solution conformation was evaluated from vicinal spin-spin coupling constants and NOE data. Despite the substitution of a proline in the sequence, [Pro-10] ANF(7-23) exhibits a considerable amount of flexibility in all of the solvents employed.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bip.360270210
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