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  • 1
    Electronic Resource
    Electronic Resource
    Recovery of alkaline proteases by membrane filtration (1992)
    Springer
    Bioprocess and biosystems engineering 7 (1992), S. 205-211 
    Details
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract This work describes the recovery of an extracellular alkaline protease from fermentation broths of a Bacillus sp ATCC 21536, at pH=10.0 using ultrafiltration (MWCO 100,000) and microfiltration (0.1 μm) membranes in hollow fiber devices. The influence of membrane pore size and polymeric material and membrane filtration performance was studied. High protein recoveries and high average flux rates were obtained with polysulfone membranes. A decrease of concentration polarization was obtained, simultaneously with enhancement of filtration flux rate and enzyme recovery by using submicron sized charged particles. These polymers lead to flocculation and adsorption of whole cells and soluble factors from the fermentation broth. The best results were obtaiend by combination of cationic (0.1%) and anionic (0.04%) polymers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00369547
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  • 2
    Electronic Resource
    Electronic Resource
    L-Serine Production from Glycine and Methanol Using Free and Entrapped Cells of Pseudomonas AM 1 (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 501 (1987), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1987.tb45736.x
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  • 3
    Electronic Resource
    Electronic Resource
    Regulation of heparinase synthesis in Flavobacterium heparinum (1982)
    Springer
    Applied microbiology and biotechnology 15 (1982), S. 252-257 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The effect of various carbon, nitrogen and sulfur sources on the production of heparinase by Flavobacterium heparinum in defined medium in the presence and absence of heparin as the inducer has been studied. Carbon catabolite repression has been observed in defined medium containing one of several carbon sources including simple sugars, alcohols and organic acids. Fed batch fermentations result in 10 g/l of cells and heparinase titers as high as 100,000 U/l by avoiding carbon catabolite repression. Growth on heparin as a sole carbon source resulted in both a high growth rate of 0.12 h−1 and a high specific activity of 18 U/mg. Specific heparinase activity was markedly reduced when the end products of heparin catabolism were used as carbon, nitrogen or sulfur sources in defined medium. In defined medium with a low sulfate concentration, of less than 10−3 M, specific activities as high as 8 U/mg have been observed even in the absence of the normally required inducer, heparin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00499966
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  • 4
    Electronic Resource
    Electronic Resource
    Penicillin amidase and penicillinase production in nitrogen- and sulfur-limited chemostats (1975)
    Springer
    Applied microbiology and biotechnology 2 (1975), S. 9-17 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The production of penicillin amidase fromBacillus megaterium ATCC 14945 was examined in a sulfur-limited chemostat. Below 50% of the maximum growth rate, enzyme production was independent of dilution rate; above this point production decreased as growth rate increased. A simple and defined medium forBacillus licheniformis 749/C was developed, and extracellular penicillinase production was studied in a chemostat under sulfur- and nitrogenlimited conditions. While the yield of this enzyme differed little between these growth-limiting conditions, the specific activity of the penicillinase per unit weight of extracellular protein was increased 50% under nitrogenlimited conditions. Furthermore, the proportion of extracellular enzyme was greater in continuous, than in batch culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01385441
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  • 5
    Electronic Resource
    Electronic Resource
    Direct monitoring of the liquid and gas phases during a fermentation in a computer-mass-spectrometer-fermentor system (1983)
    Springer
    Applied microbiology and biotechnology 18 (1983), S. 135-140 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Measurement of chemical concentrations is a weak link in the monitoring of fermentations. The use of a computer-controlled mass spectrometer (MS) has made possible the measurement of one or more volatile compounds on an essentially continuous basis, both in the liquid (broth) and the gas (headspace) phases. For our purposes, the MS was used, not as a spectrometer for chemical identification, but as a programmable detector for measuring concentrations of different compounds. Specifically, a computer-controlled MS was employed during the fermentation of Saccharomyces italicus, to monitor N2, O2, and CO2 concentration in the gas phase, and N2, O2, CO2, and ethanol in the liquid phase. The performance of the MS was carefully analyzed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00498034
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  • 6
    Electronic Resource
    Electronic Resource
    The application of dynamic calorimetry for monitoring growth ofSaccharomyces cerevisiae (1978)
    Springer
    Applied microbiology and biotechnology 5 (1978), S. 207-214 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A dynamic calorimetric technique was investigated to determine the feasibility of monitoring cell growth by thermal measurements. Theoretical analysis of growth ofSaccharomyces cerevisiae on glucose showed that the correlation depends on cellular yield values but not on ethanol formation. Experiments withS. cerevisiae on a molasses-mineral salts medium resulted in a thermal yield of 4.4 kcal/g cells, consistent with our theoretical expectations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00579341
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  • 7
    Electronic Resource
    Electronic Resource
    Effect of antifoam agents and efficiency of cleaning procedures on the cross-flow filtration of microbial suspensions (1985)
    Springer
    Biotechnology letters 7 (1985), S. 749-752 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The influence of several biocompatible antifoam agents on the performance of ultrafiltration membranes for yeast cell concentration is described. Flux rates of water solutions and cell suspensions decreased in the presence of the antifoam agents. The anti-foam fouling effect was cumulative. Water was ineffective as a cleaning substance, while 0.1 NaOH and ethanol were used successfully for membrane washing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01032290
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  • 8
    Electronic Resource
    Electronic Resource
    Influence of environmental factors in the production of R(−)-1, 2-propanediol by clostridium thermosaccharolyticum (1987)
    Springer
    Biotechnology letters 9 (1987), S. 449-454 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The effect of the most important environmental parameters, temperature, pH, gas phase composition and substrate concentration, on the production of R(−)-1,2-propanediol, was investigated. An improved yield of 0.20 g R(−)-1,2-propanediol/g glucose was reached when the fermentation was run at 60°C and pH 6.0 under a N2 atmosphere.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01027450
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  • 9
    Electronic Resource
    Electronic Resource
    Selective production of phenylalanine from phenylpyruvate using growing cells ofCorynebacterium glutamicum (1985)
    Springer
    Biotechnology letters 7 (1985), S. 93-97 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Phenylalanine was produced from phenylpyruvate by growing cells of a mutant strain ofCorynebacterium glutamicum over-producing valine. A defined medium was used to minimize the accumulation of valine. The maximum phenylalanine concentration achieved was 44.5 mM (7.5 g/l) with a phenylpyruvate molar conversion of 75%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01026675
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  • 10
    Electronic Resource
    Electronic Resource
    Measurement of heat evolution and correlation with oxygen consumption during microbial growthThis paper was presented in part at the 154th Meeting of the American Chemical Society, Chicago, III., Sept. 11, 1967. (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 269-281 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A procedure for measuring the rate of heat production from a fermentation has been developed. The method is based on measuring the rate of temperature rise of the fermentation broth resulting from metabolism, when the temperature controller is turned off. The heat accumulation measured in this manner is then corrected for heat losses and gains. A sensitive thermistor is used to follow the temperature rise with time. This procedure is shown to be as accurate as previous methods but much simpler in execution. Using this technique, the rate of heat production during metabolism was found to correlate with the rate of oxygen consumption. Experiments were performed using bacteria (E. coli and B. subtilis), a yeast (C. intermedia), and a mold (A. niger). The substrates investigated included glucose, molasses, and soy bean meal. The proportionality constant for the correlation is independent of the growth rate, slightly dependent on the substrate, and possibly dependent On the type of organism growth. This correlation has considerable potential for predicting heat evolution from the metabolism of microorganisms on simple or complex substrates and providing quantitative parameters necessary for heat removal calculations.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110302
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