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  • 1
    Electronic Resource
    Electronic Resource
    Sequence and structure of the mouse gene coding for the largest neurofilament subunit
    Amsterdam : Elsevier
    Gene 68 (1988), S. 307-314 
    Details
    ISSN: 0378-1119
    Keywords: CpG-rich island ; Intermediate filament ; intron positions ; neuron-specific gene ; phosphorylation site ; recombinant DNA
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0378-1119(88)90033-9
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  • 2
    Electronic Resource
    Electronic Resource
    Limitation to the use of electrolyte leakage for the measurement of chilling injury in tomato fruit
    Amsterdam : Elsevier
    Postharvest Biology and Technology 3 (1993), S. 103-110 
    Details
    ISSN: 0925-5214
    Keywords: Chilling injury ; Electrolyte leakage ; Tomato
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0925-5214(93)90002-K
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  • 3
    Electronic Resource
    Electronic Resource
    A specific false-positive 53 kDa signal in Western blot analysis using the enhanced-chemiluminescence system
    Amsterdam : Elsevier
    Clinical Biochemistry 27 (1994), S. 228 
    Details
    ISSN: 0009-9120
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0009-9120(94)90141-4
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  • 4
    Electronic Resource
    Electronic Resource
    Involvement of NF-Y and Sp1 in basal and cAMP-stimulated transcriptional activation of the tryptophan hydroxylase (TPH) gene in the pineal gland (2002)
    Oxford, UK : Blackwell Science Ltd
    Journal of neurochemistry 81 (2002), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The expression of the tryptophan hydroxylase (TPH) gene, encoding the rate-limiting enzyme of serotonin biosynthesis, is tightly regulated both at the transcriptional and at the post-transcriptional levels. In the pineal gland, transcription of the gene is activated in response to an intracellular circadian increase of the cAMP concentration. We have previously shown that transcription of a 2.1-kb fragment of the human TPH promoter is induced by cAMP, although it lacks the canonical cAMP responsive element, CRE. The minimal promoter (−73/+29) has only weak transcriptional activity but is responsive to cAMP. It contains an inverted CCAAT box, which was demonstrated to be involved in this response. Here, we have extended our investigation to the functional features of the inverted CCAAT box in the −252/+29 TPH promoter, which has a higher basal activity. We show that an additional cis-acting sequence, the adjacent GC-rich region, cooperates with the inverted CCAAT box for the full activation of basal transcription, and that both elements are essential for the full cAMP response. We also show that in pinealocytes, NF-Y and Sp1 transactivators bind the inverted CCAAT box and GC-rich-region, respectively. These factors participate in a novel pathway for the cAMP-mediated response of the TPH promoter, which is independent of the canonical CRE-mediated response.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1471-4159.2002.00890.x
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  • 5
    Electronic Resource
    Electronic Resource
    Effects of light intensity and CO"2 concentration on growth of banana plants (Musa AAA, cultivar 'Petite Naine') in vitro and subsequent growth following acclimatization
    Amsterdam : Elsevier
    Scientia Horticulturae 60 (1994), S. 41-54 
    Details
    ISSN: 0304-4238
    Keywords: Banana ; Micropropagation ; Musa ; Relative growth rate
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0304-4238(94)90061-2
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  • 6
    Electronic Resource
    Electronic Resource
    Embryogenic cell suspensions from the male flower of Musa AAA cv. Grand nain (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 97 (1996), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: There are very few reports on the establishment of long-term embryogenic cell cultures of banana, especially of triploid cultivars of commercial interest. Embryogenic cell suspensions were prepared using the cultivar Grand nain, the most widely grown dessert banana in the world. After culture for 5 or 6 months of immature male flowerbuds adjacent to the floral apex, yellow, compact calluses and white, friable embryogenic tissues were induced. Suspension cultures were initiated from embryogenic tissues placed in liquid medium. The packed cell volume (PCV) of the suspensions increased 2- to 5- fold with each monthly culture cycle. Plating of the embryogenic suspensions resulted in approximately 370×103 embryos per ml of PCV. Depending on the size of embryos, 3 to 20% germination was observed. A histological survey of cell suspensions and embryo development was carried out. Cellular aggregates with cells displaying typical embryogenic features were formed. Most of the somatic embryos were probably of unicellular origin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1399-3054.1996.970211.x
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  • 7
    Electronic Resource
    Electronic Resource
    Morphohistological study of the different constituents of a banana (Musa AAA, cv. Grande naine) embryogenic cell suspension (2000)
    Springer
    Plant cell reports 19 (2000), S. 748-754 
    Details
    ISSN: 1432-203X
    Keywords: Key words Banana ; Cell suspension ; Histology ; Somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Five types of cellular aggregates have been characterised in embryogenic cell suspensions of banana (Musa AAA Grande naine cv.). Type I corresponded to isolated cells or to small cell aggregates. Type II were composed of embryogenic cells. Type III can be distinguished from type II due to the presence of peripheral proliferation zones with embryonic cells. Type IV were composed of protodermic masses histologically comparable to proembryos. Type V were nodules composed of a central zone of meristematic cells and of an external zone of starchy cells. Each culture flask of a cell line contained a majority of one of the above-mentioned aggregate types. Histological studies of somatic embryo developement on semi-solid regeneration medium showed that there were close similarities between the initial steps of ontogenesis of the embryos and the different cell aggregates in liquid multiplication medium. It appeared that aggregates II–IV of the suspension belong to the same development continuum which reproduces the initial phases of somatic embryo ontogenesis on semi-solid medium. Type V resulted from the development of type IV, for which ontogenesis is hindered by direct contact with 2,4-dichlorophenoxyacetic acid and the shaken liquid multiplication medium. Type I aggregates probably do not belong to the development continuum but rather correspond to the degeneration of the other types of aggregates in the suspension. The presence of intermediate types in the liquid medium reinforces the hypothesis of a relationship between the aggregates. The aggregates tended to develop through time from a majority of type II or III at the beginning of their culture to types IV–V for older suspensions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002999900188
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  • 8
    Electronic Resource
    Electronic Resource
    Comparison of methods of liquid medium culture for banana micropropagation (1993)
    Springer
    Plant cell, tissue and organ culture 32 (1993), S. 55-60 
    Details
    ISSN: 1573-5044
    Keywords: Musa acuminata ; automation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Five different liquid medium culture methods for meristem propagation of bananas were investigated and compared with solid medium culture. Treatments studied were: gelled culture medium (treatment 1); liquid medium with immersion of the plants (treatment 2); liquid medium with cellulose culture support (treatment 3); liquid medium with partial immersion of the plants (treatment 4); liquid medium aerated by bubbling (treatment 5); liquid medium with temporary immersion of the explants for 20 min every 2h (treatment 6). After 20 days of culture, three culture groups with statistically different multiplication rates were observed: -shoots in simple liquid medium and those on cellulose substrate proliferated little or not at all, -shoots on gelled medium, those subjected to partial immersion and those in aerated medium displayed multiplication rates of 2.2 to 3.1, and -the highest multiplication rate (〉5) was observed in explants subjected to temporary immersion in the medium. Two groups of treatments differed in the accumulation of dry matter: the smallest weight (around 0.5 g) was observed in treatment 1, 2, 3 and 4, and accumulation was 2 to 5 times greater in the explants in aerated liquid medium and those subjected to temporary immersion. The highest multiplication rates and weight gains were observed in aerated treatments (treatments 4 and 5). Shoots in liquid medium continuously aerated by bubbling displayed hyperhydricity of the outer leaf sheaths. This was not observed with temporary immersion of explants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040116
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  • 9
    Electronic Resource
    Electronic Resource
    Distribution of endogenous gibberellins in dwarf and giant off-types banana (Musa AAA, cv.‘Grand nain’) plants from in vitro propagation (1995)
    Springer
    Plant growth regulation 17 (1995), S. 219-224 
    Details
    ISSN: 1573-5087
    Keywords: Gibberellin ; in vitro propagation ; Musa, quantification ; somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract GA3 and GA20 were quantified in leaf extracts from true-to-type and somaclonal variants (dwarf and giant) of Musa AAA cv. ‘Grand nain’ by GC-MS-SIM after purification on reverse- and normal-phase HPLC and detection by ELISA with GA3 antibodies and by a dwarf rice bioassay. GA3 concentration in dwarf plants was 811 ng g−1 dry weight. For normal and giant plants, the endogeneous GA3 levels were respectively 3.6 and 4.6 times higher. The GA20 concentration in the giant plant was 68 ng g−1 of dry weight. This concentration was, respectively, 4.6 and 7.3 times higher than those of normal and dwarf plants. These results suggest that the somaclonal variations affecting banana plant height are associated with modifications in GA metabolism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024729
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