ISSN:
1432-0878
Keywords:
Resident peritoneal macrophages
;
Exudate peritoneal macrophages
;
Guinea pig
;
Cell coat
;
Worm-like structures
;
Labyrinths
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary A cell coat, worm-like structures, and a previously underscribed organelle for which the name “labyrinth” is proposed, are found in both the resident and the exudate peritoneal macrophages of the guinea pig, but with what have proved to be characteristic differences in condition and amount. Cell coat and worm-like structures occurred more abundantly in exudate peritoneal macrophages, especially when the exudate was elicited by intraperitoneal injection of a hypertonic saline solution. Resident peritoneal macrophages showed only fragments of the cell coat and, occasionally, very small worm-like structures. Only these latter cells, however, have the labyrinths which, like the worm-like structures, are continuous with the plasma membrane. The cell coat and the worm-like structures were found after direct osmium fixation as well as after prefixation with glutaraldehyde followed by osmium. The labyrinths were only found after prefixation with glutaraldehyde and postfixation with osmium tetroxide. The omission of washing and centrifugation prior to fixation appeared to be of great importance for the preservation of these fine-structural specializations of the cell surface of guinea pig peritoneal macrophages.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00306785
Permalink
