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  • 1
    Electronic Resource
    Electronic Resource
    Rooting in two almond genotypes
    Amsterdam : Elsevier
    Plant Science 96 (1994), S. 163-165 
    Details
    ISSN: 0168-9452
    Keywords: Auxins ; Darkness ; In vitro ; Prunus amygdalus ; Prunus dulcis
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(94)90233-X
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    In vitro shoot regeneration from leaves of wild pear (1999)
    Springer
    Plant cell, tissue and organ culture 59 (1999), S. 1-7 
    Details
    ISSN: 1573-5044
    Keywords: cefotaxime ; histology ; leaf explant ; organogenesis ; pear ; Pyrus communis var pyraster L. ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Shoot regeneration was obtained from leaves of in vitro cultures of wild pear genotypes. The highest regeneration rates, ranging from 40% to 64%, depending on the genotype, were obtained using leaves wounded by three cuts transversely to the mid-rib, a Quoirin and Lepoivre macro-salt composition, 250 mg l-1 cefotaxime and maintaining the explants in darkness for the first 30 days (induction phase), then transferring them to an auxin-free medium in light (expression phase). A concentration of 8.8 μM BA induced the highest number of explants to produce adventitious shoots. TDZ was less effective than BA and induced hyperhydricity in regenerated shoots. The histological studies revealed that the regenerated shoots originated mainly from callus formed by epidermal and sub-epidermal cells and by cells of the vascular tissue. The regenerated shoots were micropropagated, rooted and transplanted to the soil.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006351214343
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Biochemical aspects of almond microcuttings related to in vitro rooting ability (1997)
    Springer
    Biologia plantarum 39 (1997), S. 91-97 
    Details
    ISSN: 1573-8264
    Keywords: endogenous IAA ; IAA-aspartate ; peroxidase ; phenols ; Prunus dulcis ; rhizogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Microcuttings of seven genotypes of almond (Prunus dulcis Mill) used as peach rootstocks, showed different rooting ability in vitro. No direct relationship was found between peroxidase activity and total phenol content, determined in the whole microcuttings prior to the root inducing treatment, and the rooting ability of these genotypes. However, a positive relationship was found between free indole-3-acetic acid (IAA) level and IAA-aspartate and the rooting response. After transferring into the rooting medium, peroxidase activity of the easiest-to-root genotype increase up to a peak and then declined and the total phenol content showed an opposite trend. These variations did not occur in the most difficult-to-root genotype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1000365224324
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Isolation of mRNA species related to the rooting induction in almond and apple through the differential display technique (1997)
    Springer
    Biologia plantarum 39 (1997), S. 99-104 
    Details
    ISSN: 1573-8264
    Keywords: auxin ; cDNA fragments ; gene expression ; Malus x domestica Borkh ; PCR ; Prunus dulcis Mill ; rhizogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Differential display of mRNA has been recently developed as a tool to detect and characterize changes in gene expression. We applied this technique to fruit trees plantlets induced to root in vitro, in order to isolate and study genes involved in root induction. A reproducible pattern of polymerase chain reaction (PCR) products was obtained, both in almond and apple, in vertical polyacrylamide gels stained with ethidium bromide. Differences in PCR fingerprinting were detected in mRNAs of basal part of either auxin induced or non induced microcuttings. Thus, we suggest that this technique can be used in woody species to detect changes among mRNA populations during root induction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1000317308394
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    Paper (German National Licenses)
    Fulltext
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