Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Isolation of Herpes-T virus from a spontaneous disease in squirrel monkeys (Saimiri sciureus) (1967)
    Springer
    Archives of virology 22 (1967), S. 324-331 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Herpes-T virus was isolated from 2 of 4 clinically ill squirrel monkeys. The clinical manifestations of the disease in the monkeys was characterized by oral and labial lesions. From one of two animals sacrificed for histopathological examination, Herpes-T virus was isolated from the tongue and salivary gland. Herpes-T was isolated from the oral swab of one of the two live monkeys on the day of arrival in our laboratory and again on the 4th day. The anal swabs collected from both these monkeys failed to yield virus. The sera of these monkeys collected on the day of arrival showed a neutralization index (NI) of 1.0 and 1.5 against Herpes-T virus. The convalescent sera, collected two weeks later, showed a NI of 3.5 and 4.0 respectively for the same virus. Clear plaques ranging in size from 2 to 3 mm were produced in chick fibroblast and rabbit kidney primary monolayers. This is the first report of the isolation of Herpes-T virus from naturally infected squirrel monkeys. This data further supports that the squirrel monkey is a natural host of Herpes-T virus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01242953
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Increased cancericidal activity of PTT.119, a new synthetic bis-(2-chloroethyl)amino-l-phenylalanine derivative with carrier amino acids (1984)
    Springer
    Cancer chemotherapy and pharmacology 12 (1984), S. 70-76 
    Details
    ISSN: 1432-0843
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A new synthetic tripeptide (p-F-Phe-m-bis-(2-chloroethyl)amino-Phe-Met ethoxy HCl), PTT.119, was demonstrated to have significant cancericidal activity against seven in vitro tumor cell lines of different origins and etiologies and against primary human AMML, ALL, and hairy cell leukemias. Viabilities of each murine tumor and rabbit, marmoset, and human leukemia and lymphoma line were significantly reduced by treatment with 1–50 μg PTT.119 in media containing serum. Continuous 24-h exposure or pulse treatment as short as 15 and 30 min with the tripeptide resulted in irreversible damage to the tumor cells. Under identical treatment conditions, freshly isolated human leukemic cells, particularly ALL lymphoblasts, were even more susceptible to PTT.119 than any of the tested tumor cell models. Examination of the parameters of PTT.119 activity revealed that reductions of tumor cell survival were dependent on the concentration of the tripeptide. Prolongation of PTT.119 exposure from 15 min to 24 h increased the rates of tumor cell death but did not proportionally reduce the numbers of surviving cells. Assessment of tumor cell viabilities for 5 consecutive days following pulse exposure to PTT.119 demonstrated increasing reductions in tumor cell survival, which were greatest 5 days after treatment with the tripeptide. The cancericidal activity of PTT.119 was compared with its three parental components either as individual agents or as a mixture. Both the alkylator moiety, m-sarcolysin (m.L.SL) alone or together with p-fluoro-phenylalanine and l-methionine ethoxy HCl, and l-PAM (l-phenylalanine mustard), the p-isomer of m.L.SL, were 1.5- to 3-fold less cytotoxic to L1210 leukemia and MJY-alpha mammary tumor cells than PTT.119. Covalent linkage of the amino acid residues to m.L.SL yielded a molecule with greatly augmented cancericidal activity capable of acting against a broad spectrum of tumor cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00254592
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Origins of HTLV-4 (1987)
    [s.l.] : Nature Publishing Group
    Nature 327 (1987), S. 107-107 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SIR—The recent report1 of the remarkable genetic similarity of human T-lympho-tropic virus type-4 (HTLV-4) with certain simian immunodeficiency virus (SIV) isolates from both macaques and African green monkeys (AGMs) and the accompanying editorial2 discussing the possibility of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/327107a0
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Herpes T virus plaque assay studies (1968)
    Springer
    Archives of virology 25 (1968), S. 8-17 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A plaque assay system for the study of Herpes T virus in various host cells was examined. Squirrel monkey kidney cells were found to be the most sensitive for plaque assay. The difficulty of obtaining kidney cultures without indigenous viral agents hampered the utilization of this tissue for routine purposes and favored the use of rabbit kidney cells. The optimum temperature for viral adsorption and plaque development was found to be 37°C. There was total absence of plaque development at 25° C. Addition of agar to the infected cultures without washing the monolayers prevented diffusion of virus through the agar, indicating the need for washing cell cultures when used for viral cloning procedures. Plaque yield was influenced by the time of adsorption. Maximum plaque development resulted when the adsorption time was extended to 3 hours. The influence of the inoculum size on plaque titer was paradoxical: the smaller the inoculum size (0.05 ml) the higher the plaque yield. The plating efficiency of Herpes T virus was observed by a linear relationship between virus concentration and plaque numbers. The reproducibility of replicate plaque assay and the ease with which Herpes T plaques developed in most cell cultures studied made this a reliable technique for quantitation purposes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01243085
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Herpes T virus variants (1968)
    Springer
    Archives of virology 25 (1968), S. 18-29 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Herpes T virus obtained from squirrel monkeys appears to be composed of a mixed population of large and small plaques. By clonal selection it was possible to obtain a large plaque and a small plaque strain. The latter which formed a minor component in the parent population differed from its companion large plaque type in all in-vitro markers studied: plaque size, CPE development, infectivity to host cell cultures both in degree and in titer, polykaryocyte development, rate of adsorption and thermostability characteristics. LPV measured 3 mm and SPV 1 mm as seen in rabbit kidney cell cultures 6 days post inoculation. This size difference was also seen in simian, mammalian and avian cell cultures. The CPE caused by LPV differed from SPV both in appearance and in the time of development. LPV had a greater capacity to form polykaryocytes in almost all cell cultures examined, while SPV was deficient in this regard and failed to form polykaryocytes in rabbit kidney, mouse embryo kidney, cat kidney and chick fibroblast cell cultures. LPV was more thermostable than SPV — at 56° C SPV lost 100% infectivity within 10 minutes, while LPV lost 63%. When the adsorption rates of the two clones were compared, LPV adsorbed at a faster rate with 60% being adsorbed within 15 minutes, in contrast to 31% with SPV. Plaque morphology, development of CPE, in-vitro virulence to cell cultures, formation of polykaryocytes, rate of adsorption and thermostability were useful markers for the characterization of Herpes T virus variants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01243086
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Pathogenicity studies in rabbits, hamsters, mice and embryonated eggs with herpes T virus variants (1970)
    Springer
    Archives of virology 32 (1970), S. 45-52 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Herpes T large plaque variant (LPV) and Herpes T small plaque variant (SPV) had different pathogenicity in various laboratory hosts (rabbits, hamsters, mice and embryonated eggs). LPV was found to be virulent in all these hosts while SPV was almost avirulent. SPV was found to confer immunity against the fatal infection produced by LPV in mice and hamsters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01241518
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Targeted inhibition of immunodeficiency virus replication in lymphocytes through retroviral mediated gene transfer (1993)
    Springer
    Archives of virology 133 (1993), S. 407-421 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary To test the feasibility of gene therapy for AIDS patients, an animal model is needed to evaluate the efficacy and safety of this approach. Antiviral genes (encoding antisense RNA or viral protein) derived from Simian immunodeficiency virus (SIV) were efficiently targeted into CD4+ lymphocytes through retroviral-mediated gene transfer. After challenging with infectious viruses, the transduced lymphocytes that received antiviral genes were not only protected from SIV infection, but also from infection with HIV, for at least 25 days. Furthermore, little or no cytolytic effect (syncytium formation) was observed in the protected cells. These data demonstrated that SIV or HIV replication could be effectively blocked by antisense sequence(s) or negative dominant factors which were introduced into targeted cells through retroviral-mediated gene transfer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01313779
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...