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  • 1
    Digitale Medien
    Digitale Medien
    Ultrastructure, protein synthesis and secretion of day-6 rabbit blastocysts cultured in a chemically defined, protein-free medium (1990)
    Springer
    Anatomy and embryology 182 (1990), S. 465-472 
    Details
    ISSN: 1432-0568
    Schlagwort(e): In vitro culture ; Electron microscopy ; Fluorography ; Blastocyst ; Rabbit
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Day-6 rabbit blastocysts were cultured in Ham's F10 medium supplemented with polyvinylpyrrolidone as a macromolecular component, for 4 to 12 h. The integrity of the blastocyst cells was demonstrated by electron microscopy. Expansion and biosynthesis of proteins and of DNA were studied after culturing in the presence of 35S-methionine and 3H-thymidine. Polyvinylpyrrolidone did not interfere with the subsequent protein analysis, which was performed by two dimensional gel electrophoresis followed by silver staining and fluorography. More than 600 labelled proteins were found in the blastocyst tissue, many of them were also present in the blastocyst fluid and in the blastocyst coverings. Several proteins seemed to be produced for incorporation into the blastocyst coverings; others, only detected in the culture medium, might have been synthesized for secretion into the environment.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00178911
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  • 2
    Digitale Medien
    Digitale Medien
    Uteroglobin in the developing rabbit conceptus in vivo and in vitro (1991)
    Springer
    Anatomy and embryology 184 (1991), S. 141-152 
    Details
    ISSN: 1432-0568
    Schlagwort(e): Blastocyst ; Uteroglobin ; In vitro culture ; Progesterone ; ELISA ; Fluorography ; Rabbit
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Uteroglobin (UGL) was measured in day- 4 to day-10 rabbit conceptuses by a competitive ELISA. Levels in blastocyst fluid, tissues, coverings and in the early fetus were determined separately. The total amount of UGL increased from 18.4 ng to 6.8 μg per conceptus. The UGL content of individual day-6 blastocysts was studied in vitro. Culturing was carried out up to 60 h in Ham's F10 medium with polyvinylpyrrolidone as macromolecular component, with and without progesterone, and with progesterone plus estradiol. UGL was determined in the blastocyst fluids, tissues with coverings and in the culture media. After labelling with [35S]-methionine, protein patterns of total blastocysts and of culture media were analysed by two-dimensional gel electrophoresis and fluorography. The morphology of cultured blastocysts was examined by electron microscopy. During 60 h of culture, the blastocysts expanded in diameter by 84%, and released 19% of their initial UGL content into the medium, independent of the hormonal substitution. Neither de novo synthesis, nor degradation of UGL was found: the protein remained unlabelled in fluorography, and its total quantity was not significantly different from that of non-cultured controls. Trophoblast, endoderm and embryoblast cells showed well preserved cell organelles and intercellular junctions, while the morphological differentiation of the germ layer was inhibited.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00942745
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  • 3
    Digitale Medien
    Digitale Medien
    Hemocytes of Leiobunum limbatum and two other species of harvestmen (Arachnida, Opiliones): Morphological classification and functional aspects (1987)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 193 (1987), S. 185-196 
    Details
    ISSN: 0362-2525
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: The hemocytes of Leiobunum limbatum, Mitopus morio, and Opilio ravennae number from about 8,000 (juveniles) to 41,000 (pregnant females) per microliter of hemolymph. Five different types of hemocytes occur in all three species and both sexes. According to their ultrastructural appearance and their similarities to other arthropod hemocytes these five types are designated as prohemocyte, plasmatocyte, granulocyte, coagulocyte, and spherulocyte. From the ultrastructural point of view the prohemocytes are interpreted as stem cells for plasmatocytes which on their part differentiate into granulocytes. Transitional stages which would indicate the origin of coagulocytes and spherulocytes could not be found.Granulocytes and spherulocytes are interpreted as being storage cells; coagulocytes burst when hemolymph is transferred to a microscopic slide. Plasmatocytes are involved in the removal of dead cells or cell fragments. Plasmatocytes are demonstrated as being able to phagocytize and digest bacteria.
    Zusätzliches Material: 16 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/jmor.1051930206
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  • 4
    Digitale Medien
    Digitale Medien
    Ultrastructure and function of the circulatory organs of Leiobunum limbatum and two other species of harvestmen (arachnida: Opiliones) (1986)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 190 (1986), S. 93-107 
    Details
    ISSN: 0362-2525
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: The ultrastructure of the circulatory organs of Leiobunum limbatum, Mitopus morio, and Rilaena triangularis (Arachnida: Opiliones) has been investigated. We studied the organization of the heart, the myocardial contractile elements, its tubular system, organelles, and cell-junctions. The epicardium exhibits a large number of longitudinally arranged microtubules and is attached to the myocardium by special membrane complexes. Because of these structures, the epicardium is interpreted as the elastic antagonist to the heart muscle. At both ends of the heart the epicardium continues into a nonbranching aorta. Consequently the ultrastructure of the aortal wall, containing numerous microtubules, is similar to the ultrastructure of the epicardium. At the posterior end of the heart is a muscular slit-valve, while at the anterior end is a lobe-valve that lacks contractile elements. The heart is innervated by a dorsal ganglion. It covers the entire length of the heart and contains neural and glial cells. Two different types of neurosecretory granules and both electron-dense and electron-lucent synaptic vesicles are described.
    Zusätzliches Material: 28 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/jmor.1051900109
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  • 5
    Digitale Medien
    Digitale Medien
    Uptake and accumulation of tritiated uteroglobin by day-6 rabbit blastocysts (1990)
    Springer
    Cell & tissue research 262 (1990), S. 569-577 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Blastocyst ; Uterine proteins ; Uteroglobin ; Endocytosis ; Autoradiography ; Immunocytochemistry ; Acid phosphatase ; Rabbit
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Uptake of uteroglobin (UGL) by day-6 rabbit blastocysts and the intracellular fate of this protein were studied by light- and electron-microscopic autoradiography, immunocytochemistry and acid-phosphatase cytochemistry. UGL, labelled with N-succinimidyl-(2-3-3H)-propionate, was administered to embryos in vitro for 15 min to 4 h. The kinetics, determined from light-microscopic autoradiographs, showed a continuous uptake of the labeled protein over a 4-h period of incubation. At the ultrastructural level, increasing numbers of silver grains and an intense UGL immunoreaction in protein vacuoles and crystalloid bodies of trophoblast cells indicated that 3H-UGL had accumulated in these organelles. The presence of crystalloid inclusions in protein vacuoles suggests their origin by a condensation of the protein content, including UGL. Lysosomes containing radioactivity were rarely found, suggesting a very low degradation rate of the 3H-UGL. Protein vacuoles and crystalloid bodies exhibited no acid phosphatase reaction. The enzyme was mainly found outside the basal and lateral cell membranes of trophoblast cells, and on the rough endoplasmic reticulum of endoderm cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00305254
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  • 6
    Digitale Medien
    Digitale Medien
    Uptake of tritiated uteroglobin by the endometrium of the rabbit during peri-implantation (1990)
    Springer
    Cell & tissue research 259 (1990), S. 519-528 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Endometrium ; Endocytosis ; Uteroglobin ; Autoradiography ; Rabbit (Lagomorpha)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Uteroglobin, labelled with N-succinimidyl-(2–33H)-propionate, was applied in vivo for 3 h to pregnant rabbit uteri 7 and 9 days after mating. Light- and electronmicroscopic autoradiographs showed that the endometrial epithelium, both ciliated and non-ciliated cells, is able to take up3H-uteroglobin, however, with differing intensity. Large areas of labelling were found in the luminal epithelium, whereas the glandular epithelium contained fewer silver grains. Moreover, intensively labelled single cells or symplasms occurred in both luminal and glandular epithelium. They were identified as degenerating or dead cells. After internalization by pinocytosis or phagocytosis, the tritiated uteroglobin was observed in multivesicular bodies or in lysosomes with floccular content. Later, radioactivity was either found within residual bodies or distributed throughout the entire epithelium and the subepithelial stroma, i.e., the silver grains could no longer be assigned to specific cell organelles.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01740779
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