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  • 1
    Electronic Resource
    Electronic Resource
    Electron microscopical analysis of surface charge labelling density at various stages of the erythroid line (1970)
    Springer
    The journal of membrane biology 2 (1970), S. 173-179 
    Details
    ISSN: 1432-1424
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary With use of the positively charged, colloidal ferric oxide labelling technique for electron microscopy of sections of rabbit marrow, a reduction in labelling density on the surface of differentiating red cell precursors was demonstrated. The number of iron particles per unit length of membrane was counted. A progressive diminution in labelling density follows cell division, reaching a minimum in the orthochromatic erythroblast, from which the nucleus is expelled. A slight increase in charge density is noted in the reticulocyte, and further increase is observed with its maturation to the erythrocyte. The results indicate that biosynthesis of n-acetyl neuraminic acid stops at the earliest recognizable stage of erythroid differentiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01869858
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  • 2
    Electronic Resource
    Electronic Resource
    The membrane surfaces of the toad bladder: Scanning and transmission electron-microscopy (1974)
    Springer
    The journal of membrane biology 16 (1974), S. 279-295 
    Details
    ISSN: 1432-1424
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The SEM permits analysis of large areas of the surface of epithelia and facilitates the study of cell-to-cell relationships, as pointed out by Fenguson and Heap (Z. Zellforsch.109:297, 1970). The short method of preparation described here yields good results when the critical point method is used for drying (freeze-drying was less satisfactory). Cell counts reveal that theBufo marinus toad bladder epithelium contains 3 or 4 granular (GR) cells to 1 mitochondria-rich (MR) cell. Whether these cell membrane contacts are permeable to the diffusion of high energy compounds and whether the MR cells serve as a source of energy for the GR cells are hypotheses that require further study. In view of the wide variations in the cell number per unit area even in single hemibladders, experimental measurements should probably be expressed either in terms of cell counts of DNA content, rather than per unit surface area.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01872419
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  • 3
    Electronic Resource
    Electronic Resource
    Treatment of deep sternal wound infections post-open heart surgery by application of activated macrophage suspension (2005)
    Oxford, UK; Malden, USA : Blackwell Science Inc
    Wound repair and regeneration 13 (2005), S. 0 
    Details
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Postoperative sternal wound infection remains a significant complication and generally causes considerable morbidity and mortality. Macrophages play a major role in the process of wound healing. In order to evaluate the efficacy of local injection of activated macrophage suspensions into open infected sternal wound space, a retrospective case-control study was conducted. Sixty-six patients with deep sternal wound infection treated by activated macrophages (group 1) and 64 patients with deep sternal wound infection treated by sternal reconstruction surgery with various regional flaps (group 2), were matched for gender, age, and risk index. In up to 54 months of follow-up of group 1, 60 patients (91%) achieved complete wound closure. Two (3%) late deaths occurred unrelated to the procedure. Mortality rate in group 2 was 29.7% (19/64). Duration of hospitalization was 22.6 days in group 1 vs. 56.2 days in group 2. Patients with deep sternal wound infection following open heart surgery that were treated by activated macrophages had significantly less mortality as well as significant reduction of hospitalization in comparison to the surgically treated group. These results illustrate the advantages of using a biologically based activated macrophage treatment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1067-1927.2005.130304.x
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  • 4
    Electronic Resource
    Electronic Resource
    014
Macrophage Suspensions Treatment of Infected Sternal Wounds Following Cabg Surgery (2004)
    Oxford, UK; Malden, USA : Blackwell Science Ltd/Inc.
    Wound repair and regeneration 12 (2004), S. 0 
    Details
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Macrophages serve as the coordinators of the wound healing process. Since 1998 following the Israeli Ministry of health authorization, macrophage suspensions have been used for the treatment of ulcers, in more than 800 elderly and paraplegic patients suffering from decubital chronic ulcers.As previously published, a significant number of genes showed increased levels of expression in hypo-osmotic shock activated cells, using DNA microarrays technique. The majority of these genes are considered to be directly involved in the macrophage function and in the wound healing process.Macrophge suspensions are prepared from a whole blood unit of healthy, young volunteer blood donors in a closed, sterile system, as previously described. The activated cells are applied to the wounds either by local injection or by direct deposition to the wound.Between January 2000 and October 2003, 112 patients with postoperative sternal wound infection were treated with macrophage suspension. Full closure of the wounds was achieved in 104 (93%) of the patients.〈table frame="topbot"〉〈tgroup cols="2" align="left"〉〈colspec colnum="1" colname="col1" align="left"/〉〈colspec colnum="2" colname="col2" align="left"/〉〈tbody valign="top"〉Original wound surface area8–175 cm2 (mean 93)Days until treatment6–180 (mean 47)Days until 50% closure6–60 (mean 21)Days until full closure10–138 (mean 49) No side effects were noted.The use of macrophage suspension is a safe and effective therapeutic strategy that reduces risk of complications and morbidity and improves the quality of life for long -suffering patients. Length and cost of hospital stay may be reduced, as the treatment requires no hospitalization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1067-1927.2004.0abstractxo.x
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  • 5
    Electronic Resource
    Electronic Resource
    ENDOTHELIAL SURFACE CHARGE AND ITS POSSIBLE RELATIONSHIP TO THROMBOGENESIS (1976)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 275 (1976), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1976.tb43337.x
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  • 6
    Electronic Resource
    Electronic Resource
    On the expulsion of the erythroid nucleus and its phagocytosis (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 173 (1972), S. 123-126 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Electron microscopical examination of thin sections of guinea pig and rabbit bone marrow indicates that nuclei of their normoblasts undergo constriction during their expulsion.Late erythroblasts in bone marrow of adult rabbits and guinea pigs were often seen surrounded by cytoplasmic protrusions of macrophages. No such contact was observed between macrophages and erythroblasts that were expelling their nuclei in the lumen of blood sinuses of rabbit bone marrow. It is concluded that macrophages are not necessary for nuclear expulsion.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091730111
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  • 7
    Electronic Resource
    Electronic Resource
    Fine structure of the urinary bladder of the bullfrog, (Rana catesbiana)With the financial aid of U.S.P.H.S., National Heart and Lung Institute, grant HL 06285 and NIAMD grant AM 13659. (1974)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 178 (1974), S. 15-39 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The urinary bladder of the bullfrog, Rana catesbiana has been studied by light and electron microscopy. Three epithelial cell types were found: (1) granular cells, (2) mitochondria-rich cells, and (3) basal cells. The structure of the Rana catesbiana bladder differs from that of the toad Bufo marinus, in several respects: it lacks a mucous (goblet) cell type, its granular cells do not contact the underlying basement membrane, it has specialized, smooth-muscle cell-basal epithelial cell contacts, not previously described in amphibian bladders, and its mucosa is richly innervated. Mitochondria-rich cells within the bullfrog bladder epithelium were occasionally observed touching the basement membrane. The specialized smooth muscle-basal cell contacts provide anatomical evidence for how regulatory vasoactive substances such as neurohypophyseal peptides might alter epithelial geometry. Many nerve endings invest the mucosa just beneath the epithelial basement membrane in proximity to mitochondriarich cells and basal cells. The possible role of neural regulation in epithelial transport was discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091780104
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  • 8
    Electronic Resource
    Electronic Resource
    Comparative analysis of surface charges on luminal epithelial membranes of urinary bladders from toad, frog, turtle, and tortoiseWith the financial aid of U.S.P.H.S., National Institute of Arthritis, Metabolism, and Digestive Diseases, Grant AM 13659, and National Heart and Lung Institute, Grant HL 06285. (1974)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 180 (1974), S. 509-519 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Cationized ferritin was used to analyze the surface charges on the luminal epithelial cell membranes of urinary bladders from toad (Bufo marinus), bullfrog (Rana catesbiana), turtle (Pseudemys scripta and Clemmys caspica), and tortoise (Geochelone carbonaria and Testudo graeca). The labeling, done at a physiological pH on fixed or unfixed bladders, revealed differences in the distribution and density of negative charges along the luminal membrane surfaces. The epithelial surface of toad bladder did not label with cationized ferritin. Frog bladder labeled lightly and the labeling pattern varied between cell types. The epithelial membrane surfaces of reptile bladders were heavily labeled, in contrast to amphibian bladders. Luminal surfaces from fresh water turtles were not as heavily labeled as those from land tortoises. The degree of labeling varied from cell type to cell type in all reptile bladders except Pseudemys scripta. An analysis of the degree and pattern of labeling by cationized ferritin in bladders of all species studied might reflect a difference in the nature of the glycocalyx of a particular membrane, the presence or absence of negative surface charges or their availability (i.e., interference by mucus), and/or the nature of the chemical groups comprising the surface structure of the membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091800310
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  • 9
    Electronic Resource
    Electronic Resource
    Comparative ultrastructural analysis of two tortoise bladders, Testudo graeca and Geochelone carbonariaWith the financial aid of U.S.P.H.S., National Institute of Arthritis, Metabolism, and Digestive Diseases, Grant AM 13659, and National Heart and Lung Institute, Grant HL 06285. (1976)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 184 (1976), S. 97-109 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Urinary bladders from the desert tortoises, Testudo graeca and Geochelone carbonaria were removed at specific times during the year and studied by electron microscopy. Three epithelial cell types were found in both species in all bladders examined: (1) granular cells, (2) mitochondria-rich cells, and (3) basal cells. Cells analogous to these three types have also been observed in amphibian bladders (from to ad Bufo marinus and bullfrog, Rana catesbiana) and reptilian bladders (from Pseudemys scripta and Clemmys caspica). Both tortoises have an incomplete layer of basal cells so that the granular and mitochondria-rich cells extend from the lumen to the basement membrane: some thing not observed in bladders from bullfrog or turtles. A flask-shaped light cell was observed in the Geochelone carbonaria bladder obtained in April. No counterpart of this cell was seen in the same species sacrificed in January, or in any of the Testudo graeca bladders, although a similar cell has been described in the turtle, Pseudemys scripta (Rosen, Expt. Molec. Path., 12: 286-296, 1970). This study was undertaken to characterize the cell types present in tortoise bladder and to compare them with cell types in the bladder of the turtle, bullfrog and toad.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091840108
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  • 10
    Electronic Resource
    Electronic Resource
    Age Population Distribution of Erythrocytes in Domestic Animals. An Electron Microscopic Study (1962)
    Philadelphia : Wiley-Blackwell
    Journal of Cellular and Comparative Physiology 59 (1962), S. 117-127 
    Details
    ISSN: 0095-9898
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1030590205
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