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  • 1
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Changes in water parameters were studied in a yard experiment for 7 weeks after application of cow dung at 20, 50 and 100 t ha−1, poultry manure at 4, 10 and 20 t ha−1, feed mixture (groundnut oil cake and rice bran at 1:1) at 10, 20 and 30 t ha−1 and inorganic fertilizers at 100 kg urea+50 kg single super phosphate (SSP) ha−1, twice this dose (2x ha−1) and thrice this dose (3x ha−1). To study the role of soil in the mineralization process, each treatment was divided into two groups – one with and the other without soil substrate. Higher degree of changes in water parameters was observed at higher input levels. Both organic amendment and inorganic fertilization caused significant reduction (P〈0.05) in dissolved oxygen and increase in free CO2, dissolved organic matter, total ammonia, nitrite, nitrate and phosphorus contents of water. Organic inputs significantly decreased (P〈0.05) water pH and increased total alkalinity and hardness. In contrast, inorganic fertilization caused a significant increase in pH; alkalinity and hardness increased significantly in the presence of soil, but reduced in its absence. In organic input, presence of soil substrate caused significantly lower value of pH, dissolved oxygen, dissolved organic matter and phosphate-phosphorus and significantly higher free CO2, alkalinity, hardness, ammonia, nitrite and nitrate contents, compared with those in the absence of soil, revealing enhanced microbial mineralization in the presence of soil.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A sub-lethal nitrite toxicity trial was conducted using static conditions for a period of 96 h with fingerlings of Catla catla (21.4±3.6 g). Fingerlings were exposed to five concentrations of nitrite, that is, 1, 2, 4, 8 and 10.4 mg L−1 and a nitrite-free control to study changes in haematological parameters. Nitrite caused an increase in immature erythrocyte population (7–24%) in lower concentrations (0–4 mg L−1) at 6 h while they were absent in higher concentrations. The total erythrocyte count was reduced at 6 h followed by an increase at 12 h with further reduction up to 96 h in all concentrations of nitrite. The 96-h exposure resulted in 21.2–31.8% reduction in erythrocyte population in 1–10.4 mg L−1 nitrite. The haemoglobin content decreased progressively with increasing nitrite concentrations as well as exposure periods. Total leukocyte count decreased initially at 6 h in all treatments followed by an increase after 12 h, signifying development of a protective response of the body to nitrite stress. Blood glucose decreased initially up to 24 h followed by an increase through 96 h. Serum protein level decreased continuously with increasing exposure period. The study revealed that exposure to nitrite caused changes in almost all the haematological parameters in the fingerlings depending on the concentration as well as exposure period. Nitrite being one of the important inorganic nutrients often recorded at higher levels in intensively cultured ponds, the present study highlights its adverse impact on fish and stressed the need for the management of this nutrient in culture ponds.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A comprehensive acute toxicity trial was conducted using a static water system to study the toxic effect of ammonia on haematology and enzyme profiles of Cirrhinus mrigala H. The LC50 of total ammonia-nitrogen (TAN) was 11.8 mg L−1 TAN (1.029 mg L−1 NH3-N). The sub-lethal test revealed that with increasing concentration of TAN, the total erythrocyte counts were reduced in lower concentrations (1–4 mg L−1 TAN) followed by higher levels in fish exposed to higher concentrations (8–16 mg L−1 TAN). In contrast, the total leucocyte counts were opposite. With increasing concentration of TAN, haemoglobin and serum protein content were reduced, whereas the blood glucose level increased. As the concentration of ammonia increased, there was a reduction in acetylecholinesterase activity in the brain and liver; alkaline phosphatase activity in the serum, brain and gill; and acid phosphatase (ACP) activity in the gill. The activity of lactate dehydrogenase in the gill, liver, kidney and brain increased with increased concentration of ammonia. In addition, activities of ACP in the serum and brain, alanine aminotransferase in the serum, brain and gill, and aspartate aminotransferase in the serum, brain and gill were increased.
    Type of Medium: Electronic Resource
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