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  • 1
    Electronic Resource
    Electronic Resource
    Chromatography of human immunoglobulin G on immobilized Drimarene Rubine R/K-5BL Study of mild, efficient elution procedures
    Amsterdam : Elsevier
    Journal of Chromatography A 663 (1994), S. 175-186 
    Details
    ISSN: 0021-9673
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0021-9673(94)85243-X
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Grass Pollen Hyposensitization versus Placebo Therapy (1981)
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 36 (1981), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: This study presents the pre- and post-treatment levels of specific IgE and IgG and of total IgE in the sera of two groups of grass pollen-sensitive patients, one receiving placebo injection (16 patients), the other being treated by repeated doses of a four-grass pollen extract (17 patients).Total and specific IgE were evaluated by the radioimmunological methods based on the IgE-anti-IgE interaction. IgG specific to the pollen extract was determined by a radioimmunoassay measuring protein-A bound pollen-specific antibodies (IgG). The whole immunoglobulin content specific to the allergen was quantified by a haemagglutination technique.During the treatment period, the relative variations of total IgE, and specific IgE and IgG levels are significantly different in placebo- and pollen-treated groups: 1) for total and specific IgE, were observed a relative decrease in the placebo group, and stable levels in the treated group; 2) for IgG, the level was stable in the placebo group and a relative increase was seen in the treated group.For most of the patients the specific serum IgE and IgG levels were determined also after the pollen season consecutive to the treatment. During the pollen season, the placebo group is characterized by an increase in specific IgE concentrations and stable IgG levels. The hyposensitized patients showed stable levels of specific IgE and a decrease in specific IgG during the same period.There is a significant correlation between the levels of specific haemagglutination litres and the IgG levels. After the treatment period the haemagglutination litre is significantly lower in the placebo-treated patients than in the pollen-treated ones.A significant positive correlation exists between the levels of specific IgE and IgG before and after treaiment, for the 33 patients. Moreover, during hyposensitization, the relative variations of specific IgE and IgG are highly correlated in the treated group only.No significant correlation at the P 〈 0.05 level is observed between the clinical scores and the levels of the different biological parameters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.1981.tb01583.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Local production of IgG and IgG subclasses in the aqueous humor of patients with Fuchs heterochromic cyclitis, herpetic uveitis and toxoplasmic chorioretinitis (1997)
    Springer
    International ophthalmology 21 (1997), S. 187-194 
    Details
    ISSN: 1573-2630
    Keywords: aqueous humor ; Fuchs heterochromic cyclitis ; IgG subclass ; herpetic uveitis ; toxoplasmic chorioretinitis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Changes in local immunity are important whenconsidering the physiopathology of uveitis. The aim ofthis study was to measure IgG and IgG subclassconcentrations in the serum and the AH of patients withthree different types of uveitis and to determine for eachof them the presence of a local production of IgG in theintra-ocular compartment. This investigation was extendedto IgG subclasses.Serum and AH of 46 patients, including 11 with Fuchsheterochromic cyclitis (FHC), 13 with toxoplasmicchorioretinitis, 11 with herpetic uveitis and 11 with senilecataract (taken as controls) were analyzed by ELISA forIgG and IgG subclasses. Three quotients (r1, IgG/albuminin serum; r2, IgG/albumin in AH; and R, r2/r1) werecalculated in order to estimate the local synthesis (LS) ofIgG and each IgG subclass.In AH of patients with herpetic uveitis, a concomitant andsignificant increase of all IgG subclasses as well asalbumin (with no significant increase of r2 or R) wasobserved. This finding was interpreted as an indirectconsequence of major damage to the blood–aqueousbarrier. In patients with FHC, a significant increase of r2and R involving only the IgG1 subclass was observed,indicating the existence of LS of IgG1 in the majority ofthese patients. In the AH of patients with toxoplasmicchorioretinitis, no significant modification of IgG subclassor albumin concentrations was observed when comparedto controls.In conclusion, it would seem interesting to considermeasurement of IgG and IgG subclasses and calculationof the coefficients r1, r2 and R for a better evaluation ofthe local immunological processes observed in differenttypes of uveitis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005909331778
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    Paper (German National Licenses)
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