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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Medical microbiology and immunology 178 (1989), S. 105-112 
    ISSN: 1432-1831
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Antigen A60 has been purified from the cytoplasm of Mycobacterium bovis BCG, and its composition has been determined: it has proved to be able to elicit immune reactions of both humoral and cellular type. Inoculation of A60 into the footpad of mice previously sensitized with the same antigen, or with whole mycobacterial cells produced a footpad swelling showing a peak at 24 h. Similar delayed hypersensitivity reactions were induced in sensitized guinea-pigs by subcutaneous injection of an A60 dose of 0.01 μg (minimal revealing dose). A quantity thousandfold higher (15 μg A60) was unable to induce in unsensitized guinea pigs the mounting of a cellular immunisation against A60, as shown by negative cutaneous testings 1 month later. Our results show that A60 preparations satisfied the requirements of the European Pharmacopoeia Commission and met the WHO recommandations for new tuberculins. Handicaps of old tuberculin and PPD (heterogeneous mixtures titrated biologically and unstable in solution) can be overcome by A60 preparations (a single antigen spectrophoretically measurable and stable).
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Antonie van Leeuwenhoek 40 (1974), S. 145-151 
    ISSN: 1572-9699
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We studied the relationship between ten authentic Campylobacter fetus and two C. bubulus strains, and seventeen named vibrios of animal and human origin. All organisms fall within the genus Campylobacter, as defined. Their DNA base composition ranges from 29.8 to 35.9% (G+C). On the basis of similarity of differential biochemical tests and % (G+C), they can be divided into four closely similar groups. 1. C. fetus var. intestinalis and var. venerialis with 34.7 to 35.9% (G+C). 2. Vibrio fecalis with 32.0 to 32.8% (G+C). 3. C. bubulus with 30.1 and 30.6% (G+C). 4. The cluster V. jejuni, V. coli, Vibrio sp. from fowl, and related vibrios from man and sheep with 29.8 to 34.0% (G+C). We propose that all the vibrios used here be included in the genus Campylobacter.
    Type of Medium: Electronic Resource
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