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  • 1
    Digitale Medien
    Digitale Medien
    Effects of gamma irradiation on the plasma membrane of suspension-cultured apple cells. Rapid irreversible inhibition of H+-ATPase activity (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 90 (1994), S. 0 
    Details
    ISSN: 1399-3054
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: The effects of ionizing radiation, used in post-harvest treatment of fruit and vegetables. were investigated on cultured apple cells (Pyrus malus L. cv. Royal Red) on a short-term period. Irradiation (2 kGy) induced an increase of passive ion effluxes from cells and a decrease of cell capacity to regulate external pH. These alterations are likely due to effects on plasma membrane structure and function and were further investigated by studying the effects of irradiation on plasma membrane H+-ATPase activity. Plasma membrane-enriched vesicles were prepared and the H+-ATPase activity was characterized. Irradiation of the vesicles induced a dose dependent inhibition of H+-ATPase activity. The loss of enzyme activity was immediate, even at low doses (0.5 kGy), and was not reversed by the addition of 2mM dithiothreitol. This inhibition may be the result of an irreversible oxidation of enzyme sulfhydryl moieties and/or the result of changes induced within the lipid bilayer affecting the membrane-enzyme interactions. Further analysis of the H+-ATPase activity was carried out on vesicles obtained from irradiated cells confirming the previous results. In vivo recovery of activity was not observed within 5 h following the treatment, thus explaining the decrease of cell capacity to regulate external pH.This rapid irreversible inhibition of the plasma membrane H+-ATPase must be considered as one of the most important primary biochemical events occurring in irradiated plant material.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1399-3054.1994.tb00392.x
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  • 2
    Digitale Medien
    Digitale Medien
    The Halogenation of Some Benzamides Takes Place Preferentially at the ortho, para Positions (1998)
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 1998 (1998), S. 1689-1696 
    Details
    ISSN: 1434-193X
    Schlagwort(e): Aromatic substitution ; Benzamides ; Chlorination ; Bromination ; Chemistry ; General Chemistry
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: The orientation of chlorination and bromination of N, N-disubstituted benzamides in aqueous acetic acid is strongly influenced by the nature of the alkyl groups at the nitrogen atom. With large groups, the halogenation takes place fairly selectively at the ortho/para positions.
    Materialart: Digitale Medien
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