ISSN:
1573-5001
Keywords:
Through-bond H2-H8 correlation
;
Unlabeled DNA
;
HMBC
;
Two-dimensional NMR
;
G-A base pairs
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary A new application of the HMBC experiment is presented that provides a useful means to discriminate between H2 and H8 proton resonances, to assign the base proton resonances to the various residue types and, most importantly, to correlate the H2 and H8 protons for adenine or inosine residues in natural abundance 13C fragments. The utility of this experiment is demonstrated for an unlabeled DNA 20-mer. Thanks to the obtained results, preliminary conclusions could be drawn regarding the molecular conformations of the non-canonical G/I-A base pairs in the hairpin formed by this fragment.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00211166
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