ISSN:
1432-0428
Keywords:
Diabetes mellitus
;
renal sodium transport
;
Tamm Horsfall glycoprotein
;
furosemide
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary In the present study, 8 Type 1 diabetic patients with normal creatinine clearance and 8 matched controls were examined. Tamm Horsfall glycoprotein was isolated with the NaCl precipitation procedure. Its purity was checked by gelelectrophoresis, immunodiffusion and isoelectric focussing. Tamm Horsfall glycoprotein of diabetic patients had higher glucose (p〈0.05) and lower N-acetylneuraminic acid content (p〈0.01) than controls. 14C-furosemide binding by Tamm Horsfall glycoprotein was examined using an Amicon ultrafiltration system at 0 °C. In nominally sodium-free medium, furosemide binding by Tamm Horsfall glycoprotein was significantly (p〈0.01) higher in diabetic patients than in matched controls. The increment of binding capacity with sodium was similar in controls and diabetic patients so that maximal binding capacity in a NaCl system was 1.7±0.3 in controls and 3.64±0.5 in diabetic patients (p〈0.025). Half maximal furosemide binding by Tamm Horsfall glycoprotein occured at 1.4±0.2mmol Na/l in controls and 0.52±0.12 in diabetic patients (p〈0.01). Abnormal radiofurosemide binding of Tamm Horsfall glycoprotein of diabetic patients may be the consequence of abnormal postribosomal modification of the glycoprotein which is synthesized in an insulin- and glucose-sensitive nephron segment.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00291072
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