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  • 1
    Electronic Resource
    Electronic Resource
    Primary structure of dystrophin-related protein (1992)
    [s.l.] : Nature Publishing Group
    Nature 360 (1992), S. 591-593 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Because DRP is under-represented in amplified cDNA libraries, we constructed an unamplified library using messenger RNA extracted from the human glioma cell line IN 157, in which DRP is abundantly expressed in the absence of dystrophin. Initially the library was screened with a 1.9-kb fragment ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/360591a0
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  • 2
    Electronic Resource
    Electronic Resource
    Dissociation of ‘hybrid’ isozymes on electrophoresis (1978)
    [s.l.] : Nature Publishing Group
    Nature 271 (1978), S. 84-87 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Fig. 1 Starch gel electrophoresis of SOD at 8 C and at 37 C using a Tris, EDTA, maleate, MgCl2 buffer system at /?H 7.4. Electrophoresis was carried out for 17 h at 5 V cm"1 at 8 C and 3.5V cm'1 at 37 C. Temperature was regulated during electrophoresis by circulating water at the appropriate ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/271084a0
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  • 3
    Electronic Resource
    Electronic Resource
    CAIII a marker for early myogenesis: Analysis of expression in cultured myogenic cells (1991)
    Springer
    Somatic cell and molecular genetics 17 (1991), S. 215-228 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Carbonic anhydrase III (CAIII) is an abundant muscle protein characteristic of adult type-1, slow-twitch, muscle fibers. We demonstrate that CAIII is not confined to mature muscle but is also expressed in cultured myogenic cells that were originally derived from adult and fetal limb muscle (G8 and C2C12) and by azacytidine treatment of 10T1/2 fibroblasts (23A2). Transcripts may accumulate in these cells to levels that correspond to 6.5% of that found in mature muscle. CAIII is expressed in mononucleate myoblasts and is abundant in those that preferentially fuse to form myotubes, and these findings contrast with those for many other muscle genes whose transcripts only accumulate on or after terminal differentiation. Preliminary promoter-function assays by transfection shows that 2.8 kb of sequence flanking the 5′ end of the human CAIII gene efficiently promotes transcription of the bacterial chloramphenicol acetyltransferase gene in myogenic cells. However, none of the sequences within this region are sufficient to confer muscle-specific expression. Removal of sequences 5′ to −715 bp leads to a major loss of transcriptional activity of the CAIII promoter. These results imply that the proximal CAIII promoter, which includes a putative CArG box and four potential MyoD binding sites, is not adequate for either myoblast-specific or maximal transcription.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01232818
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  • 4
    Electronic Resource
    Electronic Resource
    Characterisation of cDNA clones for rat muscle carbonic anhydrase III (1988)
    Springer
    Bioscience reports 8 (1988), S. 401-406 
    Details
    ISSN: 1573-4935
    Keywords: carbonic anhydrase ; cDNA clones ; expression library ; mRNA levels
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract cDNA clones for rat muscle carbonic anhydrase III have been isolated from a λ gt-11 library and sequenced. Comparison with human CAIII cDNA showed about 90% homology to rat. The rat clones were used to estimate mRNA from liver and muscle on Northern blots and showed that the sexual dimorphism of CAIII in rat liver relates to a difference in mRNA levels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01121636
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  • 5
    Electronic Resource
    Electronic Resource
    A radiation hybrid panel for human Chromosome 6q (1995)
    Springer
    Mammalian genome 6 (1995), S. 285-290 
    Details
    ISSN: 1432-1777
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A panel of 63 radiation-reduced hybrids has been derived from a mouse cell line containing a neo-marked human Chromosome (Chr) 6, primarily to provide a resource for higher resolution localization of new markers. Hybrids were generated with radiation doses of 40–400 Gy, selected in G418, and were shown by PCR to contain the neo gene. PCR was also used to score the retention of 15 loci that map from 6q13 to q25.2 of the current consensus map plus six other loci assigned to 6q26-q27. An average retention frequency of 27.8% was observed, with the highest frequencies at D6S313 and D6S280 (63.5%) located near the centromere at 6q13, and at D6S283 (68.5%) at 6q16.3-q21, presumably close to the neo integration site. Lowest frequencies (4.8%) were observed for telomeric markers. All markers segregated independently except D6S297 and D6S193. Agreement and some improvement to the current consensus map of 6q was made by mapping 12 loci by the non-parametric statistical method of Falk. In addition, deletion mapping with informative hybrids allowed the ordering of six loci from 6q26 to q27 and permitted some integration of maps of this region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00352418
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