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  • 1
    Digitale Medien
    Digitale Medien
    The nucleus preopticus and the nucleus lateralis tuberis in the roach, Leuciscus rutilus (1973)
    Springer
    Cell & tissue research 140 (1973), S. 369-388 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Nucleus preopticus ; Nucleus lateralis tuberis ; Leuciscus rutilus ; Falck-Hillarp technique ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The hypothalamus of the teleost fish Leuciscus rutilus was investigated with the Falck-Hillarp technique. The nucleus preopticus (NPO) and the nucleus lateralis tuberis (NLT) contain no fluorescent, i.e. catecholaminergic cells. Green fluorescent fibers probably originating from the paraventricular organ and/or the preoptic recess organ, are intermingled with the cells. The electron microscopical study was based on the three fixatives glutaraldehyde-osmium tetroxide, osmium tetroxide and potassium permanganate. In the NPO two cell types are recognized, characterized mainly by dense core vesicles (dcv) with measured diameter of 130 nm and 170 nm across respectively. The endoplasmic reticulum in the former cell type generally has large dark inclusions measuring from 175 to 375 nm across, which are also found in the neurite. In the NLT, four different cell types are identified, some of which are subject to considerable variations. The rostral and the medial parts of the nucleus include a large cell type (I) with dcv of diameter 170 nm. The medial part also has a small cell type (II) with dcv of 80 nm. The lateral part is characterized by two cell types (III, IV). Cell type III occurs in three forms with dcv of about 140 nm. The fourth cell type (IV) is rare and contains irregularly formed granules, the most circular ones measuring about 130 nm and the most elongated ones 110 nm×210 nm. The ventrolateral part contains the same cell types (except for type II) as those found in the lateral and medial parts. The morphological differentiation of the NLT as well as its different cell types strongly indicates its functional diversity. After permanganate fixation the secretory granules of the different cell types in the NPO and the NLT appear as “empty” vesicles. This method also reveals that the cell types of the two nuclei have dcv of about 90 nm. The possible monoaminergic content and the role of these dcv are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00307024
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  • 2
    Digitale Medien
    Digitale Medien
    Gonadotropic cells in the Atlantic salmon, Salmo salar (1978)
    Springer
    Cell & tissue research 191 (1978), S. 187-203 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Pituitary ; GTH-cells ; Teleost ; Immunofluorescence ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The gonadotropin-producing cells (GTH-cells) in the Atlantic salmon were studied light and electron microscopically before, during and after spawning, and after injections of luteinizing hormone-releasing hormone (LH-RH). The double immunofluorescent technique was applied using rabbit anti-carp GTH as the first antibody. Numerous immunofluorescent cells were observed throughout the pars distalis, but very few in the pars intermedia. These cells are basophilic and PAS-positive, and ultrastructurally classified as globular gonadotropes. Only one gonadotropic cell type could be identified; its size, morphology and fine structure vary considerably. In the same specimen the GTH-cells can be predominantly globular or vesicular in appearance, depending on the reproductive phase of the fish. At spawning and after LH-RH injection, many GTH-cells reach a vacuolar stage; the content of the vacuoles is not immunofluorescent. Another cell type, which resembles GTH-cells in semithin sections, did not show gonadotropic properties; its nature and functional significance are unknown. In addition, the present study revealed an increase in the synthetic and exocytotic activity of prolactin cells after LH-RH injections. It is suggested that LH-RH mediates this effect via LH and eventually via estradiol.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00222419
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  • 3
    Digitale Medien
    Digitale Medien
    Aminergic nuclei in the hypothalamus of the roach Leuciscus rutilus (1975)
    Springer
    Cell & tissue research 159 (1975), S. 493-502 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Hypothalamus ; Teleost ; Aminergic nuclei ; Falck-Hillarp method ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary In the hypothalamus of the roach (Leuciscus rutilus) green and yellow fluorescent cells were found in the nucleus recessus lateralis (NRL) and the nucleus recessus posterioris (NRP) and green fluorescent cells in the nucleus recessus preopticus (NRPO). The green fluorescence indicates the presence of noradrenaline or dopamine and the yellow one the presence of 5-hydroxytryptamine. The cells of the NRL and NRP contained electron dense granules averaging 70 nm in diameter. The NRL is divided into two parts. These and the NRP are connected by large fluorescent tracts. The NRL and NRP send axons towards the nucleus lateralis tuberis (NLT) and the NRPO sends axons towards the nucleus preopticus (NPO). It could not be established whether the aminergic nuclei described are the origin of the fluorescent fibers in the hypophysis of the roach.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00221705
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  • 4
    Digitale Medien
    Digitale Medien
    The aminergic innervation of the pituitary gland in the roach Leuciscus rutilus (1975)
    Springer
    Cell & tissue research 158 (1975), S. 169-175 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Pituitary ; Teleost ; Aminergic innervation ; Falck Hillarp technique
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Fluorescent (aminergic) fibers were demonstrated by the Falck-Hillarp technique in all parts of the neurohypophysis (NH) in the roach (Leuciscus rutilus). The fibers are very thin and few in number in the rostral (RNH) and proximal neurohypophysis (PNH) but slightly more numerous in the NH adjacent to the pars intermedia (PI). The fibers occur in normal fish as well as in specimens pre-treated with α-methyl-noradrenaline. It is proposed that all parts of the adenohypophysis have a very sparse aminergic innervation. There is no correlation between the number of type “B” fibers and fluorescent fibers in the roach NH. The technical difficulties in obtaining good and reliable results in teleost material are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00219959
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