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  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Applied microbiology and biotechnology 33 (1990), S. 26-30 
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary The yield of fibrinolytic and other proteolytic enzymes by Ca-alginate immobilized cells of Penicillium chrysogenum H9 was compared to that of free cells. The gel matrix protected the immobilized cells from lysis providing higher stability to the biocatalyst. The highest fibrinolytic activity/caseinase activity ratio was obtained in immobilized cell cultures. The composition of culture medium influenced enzyme production, showing different patterns in free and immobilized cultures. An alginate concentration of 3% and 10 ml alginate beads/50 ml medium were optimum for fibrinolytic enzyme biosynthesis. Semicontinuous production of the enzyme indicated the superiority of immobilized cells. The beads were affected by repeated exposure to phosphate ions after 12 cycles.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Applied microbiology and biotechnology 29 (1988), S. 442-446 
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary Living cells of Candida parapsilosis KSh 21 were immobilized by adsorption on different types of glass rings. The presence of n-tetradecane enhanced the cell adsorption especially on normal glass rings. The high adhesion of cellulose-coated glass rings and of sintered glass rings induced a quick adsorption of the cells. The quantity of 1-tetradecanol produced in the cultures of immobilized cells especially on SGR was higher than that of the free cells. Low numbers of free cells released in the immobilized cultures were observed. Better contact between the immobilized cells and oil droplets was noticed.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Applied microbiology and biotechnology 38 (1992), S. 312-314 
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract Acetone fractionation of Bacillus lentus culture filtrate yielded the highest α-amylase activity and the 66.6% fraction reached 13-fold that of the crude enzyme preparation. Gel filtration and ion exchange chromatography afforded a pure α-amylase (relative molecular mass, 42 000). The pure enzyme was highly active on starch and dextrin. It produced a mixture of oligosaccharides as major products of starch hydrolysis. Maximal activity was reached at 70° C and pH 6.1. Ca2+, Na+, K+ and Sr2+ ions stabilized or slightly stimulated the enzyme whereas Ag+, Co2+, Hg2+, Zn2+, Cd2+ and Fe3+ ions strongly inhibited the activity. The enzyme contained 16 amino acids, of which aspartic and glutamic acids were present in the highest proportions.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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