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  • 1
    Electronic Resource
    Electronic Resource
    Morphologic changes in white and brown adipose tissue by insulin, thyroxin and cortisol in organ cultureThis project was supported in part by N.I.H. grant HD-00142. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 167 (1970), S. 213-223 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: In order to compare the morphologic response of white and brown fat to various hormones in vitro the technique of organ culture was used in this study. Explants of white and brown fat from young mice fasted for 48 hours were exposed to culture media supplemented with insulin, thyroxin or cortisol. Morphologic changes due to these hormones were studied by means of histologic analyses and cell width measurements. Insulin acted to increase the size of cells and intracellular lipid content of brown and white fat explants when compared to similar explants exposed to unsupplemented media. Brown fat explants reacted earlier than white fat in this case. Thyroxin or cortisol alone had no influence on cell width or intracellular lipid content of these tissues. With the combination of insulin and thyroxin only white fat cells showed a greater increase in lipid deposition than that seen with insulin alone. No significant changes were noted when the combination of insulin and cortisol, or when all three hormones were added to the culture medium compared to that seen with insulin alone. The conclusion reached confirms those of other authors that brown and white fat possess enough dissimilarities to be classified as two different types of tissue.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091670209
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  • 2
    Electronic Resource
    Electronic Resource
    The influence of pituitary hormones and norepinephrine on the size of adipose cells in organ cultureThis project was supported in part by N.I.H. grant HD-00142. (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 164 (1969), S. 141-151 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The technique of organ culture was used to test the effects of various pituitary hormones and norepinephrine on the size of brown and white adipose cells of mice. The hormonal effects were measured by means of histologic analysis and cell width measurements. A concentration of 10 μg/ml of somatotropin (STH), adrenocorticotropin (ACTH) or lipotropin (LPH) was sufficient to reduce the size and content of intracellular lipid in white fat cells while a concentration of 100 μg/ml was required for similar effects on brown fat cells. Norepinephrine, at concentrations of 0.1 μg/ml or 1.0 μg/ml, was sufficient to reduce markedly the size and lipid content of both types of adipose cells. On a molar basis, these hormones acted in a similar range of concentration. The reduction in size and amount of intracellular lipid in brown and white adipose cells by STH, ACTH, and LPH were prevented when combined with 10 μg/ml of insulin. Insulin prevented the lipid depletion effect of norepinephrine in brown but not white adipose cells.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091640202
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  • 3
    Electronic Resource
    Electronic Resource
    Changes in fat cell morphology during lactation in the mouseThis investigation was supported in part by USPHS Research grants HD-00142 from the National Institute of Child Health and Human Development and CA-05388 from the National Cancer Institute. (1973)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 177 (1973), S. 533-547 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A comparison was made of changes in the morphology of fat cells in the intact mammary gland, de-epithelialized mammary fat pad, and the parametrial fat pad of female C3H mice during lactation. Mammary fat pad not in contact with actively secretory epithelium shows partial loss of fat during lactation (systemic effect) while in the presence of such epithelium there is much greater loss of fat (systemic plus local effect). In the same animal the parametrial fat pad shows greater depletion during lactation than the de-epithelialized mammary fat pad. The extent of fat depletion is dependent on the number of nursing young and the duration of lactation. In the intact mammary gland and parametrial fat pad, marked change in fat cell structure is not seen in pregnancy except possibly at the very end; after weaning, replenishment of lactation-depleted fat cells is conspicuous by 48 hours. The basic ultrastructure of mammary fat cells and the kinds of changes they undergo during depletion do not differ from those described for other mammalian adipose tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091770407
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  • 4
    Electronic Resource
    Electronic Resource
    The influence of vitamin A on the epidermisAided in part by Cancer Research Funds of the University of California. (1955)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 96 (1955), S. 419-447 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1000960305
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Human breast epithelial cells in serum-free collagen gel primary culture: Growth, morphological, and immunocytochemical analysis (1987)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 133 (1987), S. 228-234 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Human breast epithelial cells derived from various sources (fibroadenoma, reduction mammoplasty, and mastectomy tissues from premenopausal patients) have been cultured in collagen gel matrix using serum-free medium. Response to various additives has been analyzed for growth-promoting effect when added to a basal medium containing insulin, cholera toxin, and BSA. A consistent observation has been the effect of EGF and cortisol in growth stimulation of human breast epithelial cells, while separately, each additive elicited only a small response. Under this condition, employing EGF and cortisol combinations, these cells gave rise to organized colonies consisting of clusters of cells, usually spherical, without any duct-like extensions. Ultrastructural and immunocytochemical studies, using a panel of monoclonal and polyclonal antibodies, have shown that cell types and features that can be identified in the original breast tissue can also be delineated in the progeny populations. The topographical feature, consisting of lumina surrounded by a single inner layer of epithelial cells and an outer layer of basal/myoepithelial cells, can be re-created in the collagen gel system starting from small clumps of cells.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041330205
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    Paper (German National Licenses)
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