ISSN:
1471-4159
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Chloride fluxes in Synaptoneurosomes in response to additions of γ-aminobutyric acid, glycine, and ethanol were measured using a chloride-sensitive fluorescent probe 6-methoxy-N-(3-sulfopropyl) quinolinium (SPQ). The C1− gradient was directed outward by bathing cells in a medium low in C1− concentration. The Synaptoneurosomes responded to both γ-aminobutyric acid and glycine by outflow of C1− ions, as judged from an increase in SPQ fluorescence. These effects were inhibited by picrotoxin and strychnine, respectively. Ethanol also produced an outflow of C1− ions from the Synaptoneurosomes. Both picrotoxin and strychnine inhibited this effect. When the antagonists were used together, the inhibiting effect was additive. These results indicate that ethanol affects both γ-aminobutyric acid and glycine receptor-linked chloride fluxes in the rat brain.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1471-4159.1991.tb03764.x
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