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  • 1
    Electronic Resource
    Electronic Resource
    Effect of a membrane interactive peptide on plant cells of canola (Brassica napus) and two fungal pathogens (1995)
    Springer
    Plant cell reports 15 (1995), S. 115-118 
    Details
    ISSN: 1432-203X
    Keywords: Disease resistance ; Lytic peptide ; Pollen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A membrane interactive peptide was toxic to microspores, pollen and protoplasts of canola in the 1–5 µM concentration range. Similarly, at 5.0 µM the peptide completely inhibited germination of conidia ofVerticillium albo-atrum; however, when tested with conidia of a virulent isolate of blackleg (Leptosphaeria maculens), a fungal pathogen of canola, much higher levels (〉30 µM) of the peptide were required to reduce or arrest germination and growth of the conidia. When testing the relative toxicities of novel peptides on plant cells and their pathogens, pollen germination is a simple, rapid and reliable alternative to protoplasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01690266
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Haploid transformation in Brassica napus using an octopine-producing strain of Agrobacterium tumefaciens (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 831-835 
    Details
    ISSN: 1432-2242
    Keywords: Haploid ; Transformation ; Canola ; Octopine ; Agrobacterium tumefaciens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Microspore-derived embryos of Brassica napus were transformed using the disarmed octopine-producing LBA4404 strain of Agrobacterium tumefaciens containing the binary vector pBin19. Octopine-producing strains have previously been reported to be ineffective in transforming Brassica. Four actively growing yellow/ green sectors were selected from the embryos on 50 mg/l kanamycin and plants regenerated. Analysis for NPT-II activity in these young plants initially indicated no expression of the bacterial NPT-II gene. The plants were nevertheless grown to maturity, selfed and S1 seed was collected. Three of the S1 plants produced microspores which were from 4 to 20 times more tolerant to kanamycin than the original parent. Southern analysis revealed that one plant (EC-1) had a single site of insertion and the other two plants (EC-2 and EC-6) had two sites of insertion with sequence homology to the bacterial NPT-II gene. Microspores from the EC-2 and EC-6 transgenics produced embryos on approximately five times the level of kanamycin tolerated by microspores from untransformed plants, while the EC-1 transgenic produced microspores with more than 20 times the tolerance to kanamycin. Analysis of S1 progeny of the EC-1 transgenic indicated that 100% of the progeny exhibited the trait through both Southern analysis and by expressing tolerance to kanamycin in microspore-derived embryos.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00266666
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Mapping the chloroplast genome of triazine resistant canola (1986)
    Springer
    Theoretical and applied genetics 71 (1986), S. 716-723 
    Details
    ISSN: 1432-2242
    Keywords: Chloroplast DNA map ; Brassica napus ; Triazine resistance ; Canola
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The chloroplast of a triazine resistant weed biotype of Brassica campestris (bird's rape) has been transferred by repeated back-crossing into an agriculturally important strain, ‘Tower’, of Brassica napus to form a triazine resistant cultivar of canola, ‘Triton’, that is low in both erucic acid and thioglucosinolate. In this report, the B. campestris derived chloroplast chromosome of B. napus (cv ‘Triton’) has been cloned into bacterial plasmids and physically mapped for eight restriction enzymes: Apa I, Bam HI, BglI, Hind III, Pst I, Pvu II, Sac I and Xho I. The genes for rRNA, rbcL, cytF, atpA, atpB, atpE, atpH and the triazine resistance gene, psbA were located on the map by heterologous hybridization. The directions of transcription for most of these genes were determined by reverse heterologous hybridization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00263269
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Restriction patterns reveal origins of chloroplast genomes in Brassica amphiploids (1983)
    Springer
    Theoretical and applied genetics 65 (1983), S. 201-206 
    Details
    ISSN: 1432-2242
    Keywords: Brassica ; Chloroplast DNA ; Maternal inheritance ; Amphiploids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Chloroplast (ct) DNA from the three elementary Brassica species (B. nigra (L.) Koch, B. oleracea L. and B. campestris L.) and the three amphiploid Brassica species (B. carinata A. Br., B. napus L. and B. juncea (L.) Czern.) was digested with fifteen restriction endonucleases. In all species restriction sites for enzymes with GC-rich recognition sequences were less frequent and not as variable as for those with AT-rich sequences. Comparisons between species revealed two distinct groups of ct DNA fragment patterns: complex one, composed of B. oleracea, B. napus, B. campestris and B. juncea and complex two, composed of B. nigra and B. carinata. The patterns of B. carinata were virtually identical to those of B. nigra and those of B. juncea were virtually identical to those of B. campestris indicating not only where the ct genomes of B. carinata and B. juncea originated, but also how little these genomes have been altered since the origin of these amphiploids. Ct DNA in B. napus shows more homology with that of B. oleracea than with that of B. campestris, but the ct genome of this amphiploid has diverged more from that of its putative parent than have those of the other two amphiploids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00308066
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    Paper (German National Licenses)
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