ISSN:
1365-2958
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
,
Medicine
Notes:
Two nodulation regions from the symbiotic plasmid (pSym) of Rhizobium phaseoil CE-3 were identified. The two regions were contained in overlapping cosmids pSM927 and pSM991. These cosmids, in a R phaseoli pSym-cured strain background, induced ineffective nodules on Phaseolus vulgaris roots.Transconjugants of Rhizobium meliloti harbouring pSM991 induced nodule-like structures on bean roots, suggesting that this cosmid contains host-range determinants.Analysis of deletions and insertional mutations in the sequences of pSM991 indicated that the genes responsible for the induction and development of nodules in P. vulgaris are organized in two regions 20kb apart. One region, located in a 6.8kb Eco RI fragment, includes the common nodABC genes. The other region, located in a 3.5kb EcoRI fragment, contains information required for host-range determination.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2958.1989.tb00237.x
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