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  • 1
    Electronic Resource
    Electronic Resource
    6-Azauracil inhibition of GTP biosynthesis in Saccharomyces cerevisiae (1992)
    Springer
    Current genetics 22 (1992), S. 9-11 
    Details
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; IMP dehydrogenase ; 6-azauracil ; GTP level
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The addition of 6-azauracil to the growth medium causes a strong reduction of the GTP level in the nucleotide pool of Saccharomyces cerevisiae. In-vitro experiments show a strong inhibition of IMP dehydrogenase activity by 6-azaUMP explaining the preceeding effect. PPR2 mutants, previously characterized by an increased sensitivity to 6-azauracil compared to the wildtype, are specifically susceptible to the lowering of the GTP pool, and are able to grow in presence of 6-azauracil when guanine is added to the medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00351735
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    S-adenosyl methionine requiring mutants in Saccharomyces cerevisiae: Evidences for the existence of two methionine adenosyl transferases (1978)
    Springer
    Molecular genetics and genomics 163 (1978), S. 153-167 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mutants requiring S-adenosyl methionine (SAM) for growth have been selected in Saccharomyces cerevisiae. Two classes of mutants have been found. One class corresponds to the simultaneous occurrence of mutations at two unlinked loci SAM1 and SAM2 and presents a strict SAM requirement for growth on any medium. The second class corresponds to special single mutations in the gene SAM2 which lead to a residual growth on minimal medium but to normal growth on SAM supplemented medium or on a complex medium like YPGA not containing any SAM. These genetic data can be taken as an indication that Saccharomyces cerevisiae possesses two isoenzymatic methionine adenosyl transferases (MAT). In addition, SAM1 and SAM2 loci have been identified respectively with the ETH-10 and ETH2 loci previously described. Biochemical evidences corroborate the genetic results. Two MAT activities can be dissociated in a wild type extract (MATI and MATII) by DEAE cellulose chromatography. Mutations at the SAM1 locus lead to the absence or to the modification of MATII whereas mutations at the SAM2 locus lead to the absence or to the modification of MATI. Moreover, some of our results seem to show that MATI and MATII are associated in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267406
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    A regulatory interaction between pyrimidine and purine biosyntheses via ureidosuccinic acid (1974)
    Springer
    Molecular genetics and genomics 133 (1974), S. 63-75 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary l-Ureidosuccinic acid (USA), an intermediate metabolite of pyrimidine biosynthesis, inhibits cell growth. Mutants blocked in pyrimidine biosynthesis after dihydroorotate (DHO), are more resistant to USA than are the wild type and the mutants blocked in the synthesis of DHO. This difference in sensitivity correlates with the level of dihydroorotase activity. Purines revert USA inhibition. Mutants selected for their resistance to USA were resistant because of a) overproducing purines (adenine excretion), b) having a reduced USA uptake, or c) showing a USA resistance independant of the above phenotypes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268678
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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