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  • 1
    Electronic Resource
    Electronic Resource
    Protein Degradation by Lactobacillus plantarum and Lactobacillus casei in a Sausage Model System (2002)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 67 (2002), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : :The proteolytic activity of a starter culture involving Lactobacillus plantarum and Lactobacillus casei towards meat sarcoplasmic and myofibrillar proteins during the fermentation of a sausage-like system was studied. After 96 h of incubation the proteolytic system of L. plantarum CRL681 caused a degradation of both sarcoplasmic and myofibrillar proteins, whereas L. casei CRL705 showed a different affinity to meat proteins. The inoculation of both strains showed a higher activity toward sarcoplasmic fraction. These results correlated with a high rate of sarcoplasmic protein degradation observed in SDS-PAGE analysis. The generation of free amino acids as well as the pH drop at the end of the incubation period was maximal in presence of the mixed starter culture, thereby demonstrating the suitability of these strains to be used in the fermentation of meat products.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.2002.tb09473.x
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  • 2
    Electronic Resource
    Electronic Resource
    Decarboxylase activity involved in methyl ketone production by Staphylococcus carnosus 833, a strain used in sausage fermentation (2002)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 210 (2002), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Staphylococcus carnosus strain 833, inoculated into sausage, increased the levels of methyl ketones which contributed to the cured aroma. These ketones were predicted to arise from incomplete β-oxidation followed by a decarboxylation. To check this hypothesis, we measured the β-decarboxylase activity in resting cells of S. carnosus grown in complex or in synthetic medium, using as substrate a β-ketoacid, which can be an intermediate of the β-oxidation pathway. This activity was present throughout the growth period. The enzyme appeared to be constitutive because no induction was observed. High aeration, a pH of 5 and the presence of nitrate promoted the production of methyl ketones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2002.tb11182.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Definitive localization of Becker muscular dystrophy in Xp by linkage to a cluster of DNA polymorphisms (DXS43 and DXS9) (1985)
    Springer
    Human genetics 〈Berlin〉 71 (1985), S. 33-36 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A study of linkage between Becker muscular dystrophy and four X chromosome-specific DNA polymorphisms in 17 kindreds has indicated that this gene is located in Xp, as already anticipated by single pedigree analysis. In particular the DXS43 and DXS9 loci, identified by probes D2 and RC8, respectively, are closely linked to each other and are both located at approximately 15 cM from the Becker locus. These linkage data, together with the previously established linkage between Becker and the DXS7 locus identified by probe L 1.28, indicate that the Becker gene is located in the same region where Duchenne has been mapped and also yield information about relative genetic distances among different DNA polymorphisms of the X chromosome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00295664
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    A common disinfectant used in condom processing inhibits endonuclease digestion of sperm DNA (1991)
    Springer
    International journal of legal medicine 104 (1991), S. 281-284 
    Details
    ISSN: 1437-1596
    Keywords: Sperm DNA ; Endonuclease digestion ; Dibenzalkonium chloride ; Sperma-DNA ; Endonucleasen-Verdau ; Dibenzalkoniumchlorid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung DNA, welche von einem an einem Tatort gefundenen Kondom gewonnen wurde, erwies sich als unverdaulich mit Restriktionsenzymen. Hierdurch wurde die Charakterisierung mit Southern Blot und Hybridisierung mit einer polymorphen Probe verhindert. Mehrere chemische Substanzen, welche bei der Herstellung und Behandlung der Kondome benutzt werden, wurden auf ihre inhibitorischen Eigenschaften gegenüber Restriktionsenzymen getestet. Insbesondere erwies sich Dibenzalkoniumchlorid als wirksam, die Enzyminhibition bei sehr niedrigen Konzentrationen zu verursachen. Die Effektivität von Maßnahmen, um die Schneidbarkeit der Proben-DNA bei Gegenwart solcher Kontaminierungen wieder herzustellen, wird diskutiert.
    Notes: Summary DNA recovered from a condom found at a crime scene appeared undigestible with restriction enzymes, preventing characterization by Southern blot and polymorphic probe hybridization. Several chemical substances used in the processing and treatment of condoms were tested for inhibitory action of restriction enzymes. In particular dibenzalkonium chloride appeared to promote enzyme inhibition at very low concentrations. The effectiveness of treatments to restore cleavage of sample DNA in the presence of such contaminants is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01369586
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    Paper (German National Licenses)
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