ISSN:
1573-6784
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary A simple, efficient method was used to purify PCR primers which had degraded during storage at-20°C/-9°C. The primers were eiectrophoresed on 3% (w/v) agarose gel, the main band was electroeluted via a trough cut in the gel. The primers were recovered by isobutanol extraction followed by ethanol precipitation. The yield was 20–40% and the A260/A280 ratio was greater than 1.8. The purification resulted in good amplification.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00155498
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